Development of spectrofluorimetric method for determination of certain aminoglycoside drugs in dosage forms and human plasma through condensation with ninhydrin and phenyl acetaldehyde

Omar, Mahmoud A.; Hammad, Mohamed A.; Nagy, Dalia M.; Aly, Alshymaa A.

doi:10.1016/j.saa.2014.10.079

Cited by 39 publications

(25 citation statements)

References 33 publications

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“…On the other hand, spectrouorimetric methods have the advantages of being simple, highly sensitive and time saving technique. [12][13][14][15][16] To our knowledge, there is no reported spectrouorimetric method for determination of salmeterol xinafoate up till now. In this study, we investigate the reaction of salmeterol xinafoate that contains a basic secondary amine with NBD-Cl which has been widely used for the determination of many pharmaceuticals containing primary and secondary amino groups [17][18][19][20][21][22] in order to develop new, simple, sensitive and rapid spectrouorimetric method for determination of the studied drug in its dosage form, spiked plasma and in the presence of its degradation products.…”

Section: Introductionmentioning

confidence: 99%

Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

2017

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Section: Introductionmentioning

confidence: 99%

Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

2017

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“…Ninhydrin and an aldehyde were widely used as a reagent that reacts with primary amines to give colored fluorescent derivatives (Fig. ) . The use of phenylacetaldehyde together with ninhydrin showed a higher increase in fluorescence intensity of the product than the use of any other aldehyde .…”

Section: Resultsmentioning

confidence: 99%

Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma

2018

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For the determination of heptaminol (HEP) in its authentic and dosage form as well as in human plasma, a new simple, sensitive and cheap fluorimetric method of analysis was developed and validated. The presented method is based on the reaction between aliphatic primary amino moiety present in HEP with ninhydrin and phenylacetaldehyde using Torell and Stenhagen buffer at pH 8.2 that yields a highly fluorescent derivative which after excitation at 390 nm showed a fluorescence emission at 464 nm. The effects of various experimental factors on both the development and stability of the fluorescent product was evaluated and optimized. In the concentration range (0.5-6.0 μg/ml), the constructed calibration curve was linear with a good correlation coefficient (0.9997) and the calculated limit of detection (LOD) and limit of quantitation (LOQ) were 0.14 and 0.43 respectively. The presented method was successfully applied for determination of Corasore® tablets and validated according to ICH guidelines.

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“…The reaction stoichiometry was examined by the mole ratio method using equimolar concentrations of both SAL and EAA (1.0 × 10 −3 M). The reaction was found to proceed at 1:1 ratio between SAL: EAA.…”

Section: Resultsmentioning

confidence: 99%

Utility of Von Pechman synthesis of coumarin reaction for development of spectrofluorimetric method for quantitation of salmeterol xinafoate in pharmaceutical preparations and human plasma

et al. 2018

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Simple, precise and selective spectrofluorimetric technique was evolved for quantitation of selective β agonist drug namely salmeterol xinafoate (SAL). Utilizing its phenolic nature, a method was described based on the reaction of the studied drug with ethyl acetoacetate (EAA) to yield extremely fluorescent coumarin product which can be detected at 480 nm (λ = 420 nm). The procedure obeys Beer's law with a correlation coefficient of r = 0.9999 in the concentration range between 500 and 5000 ng ml with and 177 ng ml for limit of detection (LOD) and limit of quantification (LOQ), respectively. Diverse reaction variables influencing the firmness and formation of the coumarin product were accurately examined and modified to ensure greatest sensitivity of the procedure. The proposed technique was performed and examined according to the US Food and Drug Administration (FDA) guidelines for bio-analytical methods and was efficiently applied for quantitation of SAL in both pharmaceutical preparations (% recovery = 100.06 ± 1.07) and spiked human plasma (% recovery = 96.64-97.14 ± 1.01-1.52).

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Development of spectrofluorimetric method for determination of certain aminoglycoside drugs in dosage forms and human plasma through condensation with ninhydrin and phenyl acetaldehyde

Cited by 39 publications

References 33 publications

Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma

Utility of Von Pechman synthesis of coumarin reaction for development of spectrofluorimetric method for quantitation of salmeterol xinafoate in pharmaceutical preparations and human plasma

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