Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma

Abstract

For the determination of heptaminol (HEP) in its authentic and dosage form as well as in human plasma, a new simple, sensitive and cheap fluorimetric method of analysis was developed and validated. The presented method is based on the reaction between aliphatic primary amino moiety present in HEP with ninhydrin and phenylacetaldehyde using Torell and Stenhagen buffer at pH 8.2 that yields a highly fluorescent derivative which after excitation at 390 nm showed a fluorescence emission at 464 nm. The effects of various experimental factors on both the development and stability of the fluorescent product was evaluated and optimized. In the concentration range (0.5-6.0 μg/ml), the constructed calibration curve was linear with a good correlation coefficient (0.9997) and the calculated limit of detection (LOD) and limit of quantitation (LOQ) were 0.14 and 0.43 respectively. The presented method was successfully applied for determination of Corasore® tablets and validated according to ICH guidelines.

Applicability of ninhydrin as a fluorescent reagent for estimation of teicoplanin in human plasma using salting‐out assisted liquid–liquid extraction technique

“…Due to its reproducibility, accuracy, and low cost, ninhydrin is a widely used derivatizing reagent for many primary amine-containing drugs for spectrophotometric and/or spectrofluorimetric analysis. [12][13][14] In this study, ninhydrin was reacted with the primary amine groups in TEIC in the presence of phenylacetaldehyde to produce a fluorogenic product; this product was measured at λ ex 390 nm and λ em 460 nm as excitation and emission wavelengths, respectively, as described in Figure 1(a).…”

“…The mechanism and stoichiometry of the reaction between ninhydrin and TEIC were at a ratio of 1:1, as shown in Figure 1(b); this finding agrees with the results from previously reported methods. [12,14]…”

“…Ninhydrin, a derivatizing reagent was reacted with primary amine moiety-containing drugs for their spectrofluorimetric analysis to form highly sensitive fluorescence products. [12][13][14] In addition, salting-out assisted liquid-liquid extraction (SALLE) technique was used to extract TEIC from human plasma samples. SALLE is a simple and effective extraction method based on liquid-liquid extraction (LLE), in which high concentrations of salt are added to separate the aqueous phase from the partially miscible organic phase, during which the target compound is extracted into the separated organic phase.…”

Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma

“…[1] Various methods have been reported for determining heptaminol in its dosage forms and in plasma. Spectrophotometric [2][3][4] , spectrofluorimetric [4][5][6][7][8] , chromatographic [9][10][11][12][13][14][15][16][17][18][19][20][21][22]] and capillary electrophoretic [23]…”

Simple ultrasensitive spectrofluorimetric method for determination of midodrine in its tablet form: Application to content uniformity testing

“…[1][2][3][4][5] A survey of the relevant literature on midodrine found that it had been quantified in its pharmaceutical preparations or in biological fluids via a variety of analytical approaches that included chromatographic, [6][7][8][9][10][11][12][13][14] spectrophotometric, [15][16][17][18][19] electrochemical [20] and spectrofluorimetric [21] techniques. [1][2][3][4][5] A survey of the relevant literature on midodrine found that it had been quantified in its pharmaceutical preparations or in biological fluids via a variety of analytical approaches that included chromatographic, [6][7][8][9][10][11][12][13][14] spectrophotometric, [15][16][17][18][19] electrochemical [20] and spectrofluorimetric [21] techniques.…”