Development of protein-recognition SPR devices by combination of SI-ATRP with biomolecular imprinting using protein ligands

Abstract

Abstract:Molecularly imprinted polymer brush layers and gel layers with both a lectin (ConA) and an antibody-IgG as biomolecular ligands for a target protein were formed on surface plasmon resonance (SPR) sensor chips via surface-initiated atom transfer radical polymerization (SI-ATRP) without and with a crosslinker, respectively. While the IgG-imprinted brush layers chip had almost the same affinity constant for target IgG as the nonimprinted brush layer chip, the affinity constant of the IgG-imprinted gel layer chip was approximately twice than that of the nonimprinted gel layer chip. These indicate that chemical crosslinks are very important factor to create distinct molecular recognition sites by molecular imprinting. Thus, biomolecular imprinting that uses biomolecular ligands and crosslinkers enables us to design polymer layer chips with distinct molecular recognition sites with a strong affinity for a target biomolecule. The molecularly imprinted gel layers chips with lectin and antibody ligands are promising candidates for fabricating SPR sensor systems to monitor target biomolecules such as proteins.

Preparation of IgG imprinted polymers by metal-free visible-light-induced ATRP and its application in biosensor

“…For example, Yildirim et al [ 26 ] designed myoglobin imprinted polymer films on silicon wafers by surface-initiated ATRP. Naraprawatphong et al [ 27 ] successfully synthesized IgG-recognition SPR devices by combination of surface-initiated ATRP with imprinted polymers. Gai et al [ 28 ] developed a bovine serum albumin imprinted magnetic polymer based on ATRP method for protein separation.…”

Design of molecularly imprinted hydrogels with thermoresponsive drug binding sites

“…Additionally, a few researchers created molecular binding sites within weakly cross-linked hydrogels that had been swollen in an aqueous medium. [32][33][34][35][36][37][38][39][40][41] Furthermore, thermoresponsive PNIPAAm-based hydrogels can be used to separate hydrophobic chemicals, since the temperature-dependent conversion of the PNIPAAm chains to hydrophobicity enhances their hydrophobic interaction with hydrophobic molecules. 42,43 These PNIPAAm-based hydrogels preferentially adsorb hydrophobic molecules by hydrophobic interactions above their transition temperature but do not adsorb below their transition temperature.…”

“…In contrast to conventional molecular imprinting, which uses a large amount of cross-linker, we have prepared molecularly stimuli-responsive hydrogels that change their volume by recognizing a target molecule via molecular imprinting that uses a minute amount of cross-linker and molecular complexes as dynamic cross-links. [32][33][34][35][36][37][38][39] For example, tumor-marker-responsive hydrogels that shrank according to the concentration of a tumor marker glycoprotein were prepared by biomolecular imprinting using antibodies and lectins as ligands. 32,33 Molecularly imprinted hydrogels with b-cyclodextrin (CD) as a ligand also shrank in response to the target bisphenol A (BPA), which has a high possibility of being an endocrine disrupting chemical, because the formation of a 2 : 1 sandwich-like molecular complex between CD and BPA increased the crosslinking density.…”

Improvement of Adsorption Selectivity of Ion-Exchange Chromatography through the Double-Recognition Mode

“…In addition, the IF was in the range of 2.8−4. Naraprawatphong et al 43 fabricated an IgG-imprinted gel layer chip and the IF was around 2. The performance of DEAE@bIgG could be comparable with those products.…”