Development of platelet secretory granules

King, Samuel T.; Reed, Guy L.

doi:10.1016/s1084952102000599

Cited by 124 publications

(114 citation statements)

References 96 publications

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“…Physiologically, activated platelets are known to deliver a broad spectrum of growth factors and other active molecules by exocytosis at the site of injury. the growth factors released from α-granules include platelet-derived growth factor (PDGF), epithelial growth factor (eGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and transforming growth factor β1 (TGF-β1) (King and Reed, 2002;Maguire and Fitzgerald, 2003;Reed et al, 2000;Rendu and Brohard-Bohn, 2001).…”

Section: Cell Extractionmentioning

confidence: 99%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

177

149

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Section: Cell Extractionmentioning

confidence: 99%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

177

149

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“…Examples include melanosomes in vertebrate melanocytes and pigment granules in Drosophila retinal pigment cells (Lloyd et al, 1998;Marks and Seabra, 2001;Raposo and Marks, 2002). Mammalian cytotoxic T lymphocytes, basophils, and platelets each have secreted lysosomes whose contents initiate and modulate immune and clotting responses (Blott and Griffiths, 2002;King and Reed, 2002;Stinchcombe et al, 2004). Several human diseases are associated with defects in the formation or function of these specialized lysosomes, such as HermanskyPudlak syndrome (HPS), Chediak-Higashi syndrome, and Griscelli syndrome (Stinchcombe et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of Lysosomal Trafficking inCaenorhabditis elegans

Hermann

Schroeder

Hieb

et al. 2005

MBoC

258

464

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The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells. INTRODUCTIONLysosomes are ubiquitous membrane-bound organelles that function as major degradative sites within eukaryotic cells (Tappel, 1969). Lysosomes contain an assortment of aciddependent hydrolases that function in the breakdown of proteins, lipids, nucleic acids, and oligosaccharides. Lysosomes receive exogenous material through the endocytic pathway and are characterized as being the terminal compartment of the endocytic pathway. Lysosomes also receive material via the secretory pathway and directly from the cytoplasm (Kornfeld and Mellman, 1989;Mullins and Bonifacino, 2001;Luzio et al., 2003). Lysosomes function in diverse and important cellular processes including cell surface receptor turnover, destruction of pathogens, antigen processing, digestion, starvation responses, tissue remodeling, ion storage, autophagy, and plasma membrane repair.The yeast vacuole shares several characteristics with the lysosomes of higher animals. Genetic screens have led to the identification of Ͼ150 genes necessary for the transport and sorting of newly synthesized proteins to the yeast vacuole (Jones, 1977;Bankaitis et al., 1986;Rothman and Stevens, 1986;Bonangelino et al., 2002). These genes control two pathways of Golgi-to-vacuole transport, the carboxypeptidase Y (CPY) and alkaline phosphatase (ALP) sorting pathways (Burd et al., 1998;Conibear and Stevens, 1998;Mullins and Bonifacino, 2001). Proteins trafficked via the CPY pathway transit an endosomal prevacuolar compartment en route to the vacuole. The ALP pathway mediates transport to the vacuole independent of the prevacuolar compartment.Many of the genes involved in transport to the yeast vacuole have homologues in higher animals (Lemmon and Traub, 2000;Mullins and Bonifacino, 2001;Bonangelino et al., 2002). For example, the HOPS complex proteins (Vps11p, Vps16p, Vps18p, and Vps33p) regulate membrane fusion events necessary for lysosomal delivery within yeast (Rieder and Emr, 1997;Peterson and Emr, 2001), Drosophila melanogaster (Sevrioukov et al., 1999;Sriram et al., 2003), and mammalian (Poupon et al., 2003;Richardson et al., 2004) endosomal systems. Similarly, the proteins compos...

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“…15 Amongst others, the a-granules may release growth factors such as PDGF, transforming growth factor-beta (TGFB), IGF1, platelet-derived angiogenesis factor (PDAF), VEGF, and epidermal growth factor (EGF). 16,17 In addition, chemokines are released like CCL3, CCL5, CCL 7, CCL 17, or CXCL 1 and CXCL 12 that may lead to the migration of progenitor cells from the bone marrow. 18 In cartilage formation or chondrocyte differentiation, TGFB is known to induce chondrogenesis of mesenchymal progenitor cells, while PDGF helps chondrocytes to maintain the Additional supporting information may be found in the online version of this article.…”

mentioning

confidence: 99%

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

Krüger

Hondke

Endres

et al. 2011

Journal Orthopaedic Research

173

124

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In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96-well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1-100% PRP significantly (p < 0.05) stimulate the migration of CSP compared to untreated controls. Histological staining of proteoglycan and immuno-staining of type II collagen indicated that progenitors stimulated with PRP show significantly increased cartilage matrix formation compared to untreated progenitors. Real-time gene expression analysis of typical chondrocyte marker genes as well as osteogenic and adipogenic markers like osteocalcin and fatty acid binding protein showed that PRP induces the chondrogenic differentiation sequence of human progenitors in high-density pellet cultures, while osteogenic or adipogenic differentiation was not evident. These results suggest that human PRP may enhance the migration and stimulate the chondrogenic differentiation of human subchondral progenitor cells known from microfracture. ß

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Development of platelet secretory granules

Cited by 124 publications

References 96 publications

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Genetic Analysis of Lysosomal Trafficking inCaenorhabditis elegans

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

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