Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch, Caroline

doi:10.14573/altex.2011.4.305

Cited by 176 publications

(177 citation statements)

References 71 publications

(66 reference statements)

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“…We therefore appeal to cell and tissue culturists to reduce or completely avoid FBS in their cultures, e.g. by alternatives to FBS, like serum-free cell and tissue culture , Gstraunthaler 2003Gstraunthaler and Lindl 2013), or the replacement of FBS by the use of serum substitutes (Rauch et al 2011). Especially, cultures that will be initiated in the future, like induced pluripotent stem cells (iPS) (www.…”

mentioning

confidence: 99%

A plea to reduce or replace fetal bovine serum in cell culture media

2013

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mentioning

confidence: 99%

A plea to reduce or replace fetal bovine serum in cell culture media

2013

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“…With hPL, bulk thrombocytic growth factors are added to basal culture media, providing a human-based, xeno-free culture system (Gstraunthaler et al, 2015;Rauch et al, 2011). hPL are superior over other human-based alternatives for several reasons.…”

Section: Human Platelet Lysates (Hpl) 411 Hpl In Animal and Human Cmentioning

confidence: 99%

“…The mitogenic potential of thrombocytic growth factors was determined by specific stimulation of the ERK1/2 MAP kinase signaling cascade in quiescent renal epithelial LLC-PK 1 cultures (Rauch et al, 2011). Also, its effect on renal epithelial cell differentiation endpoints was compared to that of FBS.…”

Section: Alternatives To Fbsmentioning

confidence: 99%

Fetal bovine serum (FBS): Past – present – future

Valk

Bieback

Buta³

et al. 2018

ALTEX

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“…Como recurso alternativo ao uso de soro bovino no cultivo celular, Rauch et al (2011) sugerem o uso de plaquetas lisadas humanas, obtidas de sangue humano proveniente de bancos de sangue de doadores voluntários. O uso das plaquetas humanas, além de ser mais econômico, apresenta um grande potencial no crescimento das linhagens celulares, assim como minimiza o risco de contaminações microbianas e reduz reações imunológicas.…”

Section: Cultivo Celular E Histológicounclassified

Propostas pedagógicas substitutivas ao uso de animais no ensino superior: uma revisão

Carniatto

2017

Pubvet

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RESUMO.Animais são utilizados em atividades de ensino e pesquisa há vários séculos, principalmente em estudos relacionados à anatomia, fisiologia e recentemente à farmacologia; são, também, utilizados em aulas práticas e em atividades didáticas de diversos cursos de graduação. Entretanto, com a crescente discussão sobre os direitos dos animais não humanos, e cientes da responsabilidade do homem na conservação do planeta e na manutenção da biodiversidade, se faz necessário desenvolver recursos alternativos ao uso de animais no ensino e na pesquisa no nível superior. Este estudo teve como objetivo investigar o uso de métodos substitutivos à experimentação animal, através de consultas a periódicos nacionais e internacionais relacionados à bioética, experimentação animal, vivissecção e recursos alternativos. Foram encontrados métodos substitutos à experimentação animal, que podem ser aplicados em cursos superiores no Brasil, sobretudo em estudos e pesquisas biomédicas, farmacêuticas e toxicológicas. O uso de animais em atividades práticas, onde estes são sacrificados apenas para observação de estruturas anatômicas ou visualização de processos fisiológicos já conhecidos e estabelecidos, pode ser substituído por gravações e/ou modelos anatômicos. O uso de recursos alternativos é um processo tecnológico, porém lento, que deve ser desenvolvido, implantado e incentivado pelo professor universitário, afirmando sua responsabilidade social e científica.Palavras chave: Bioética, didática no ensino superior, experimentação animal Pedagogical proposals for the use of animals in higher education: a review ABSTRACT. Animals are used in teaching and research activities for centuries, especially in studies related to anatomy, physiology and, recently, pharmacology; they are also used in practical classes and educational activities of undergraduate courses. However, with the growing discussion on the rights of nonhuman animals, and aware of the responsibility of man in the preservation of the planet and in maintaining biodiversity, it is necessary to develop alternative resources to the use of animals in teaching and research in higher education. This study aimed to investigate the use of substitutive methods to animal testing, consultation national and international journals related to bioethics, animal experimentation, vivisection and alternative resources. There are substitute's methods to animal testing, which can be applied to higher education in Brazil, especially in education and biomedical, pharmaceutical and toxicological researches. The use of animals in practical activities where they are sacrificed just to observe anatomical structures or visualization physiological processes that are already known and established, can be replaced by recordings or anatomical models. The use of alternative resources is a technological process, though slow, which should be developed, deployed, and encouraged by the professor, saying his social and scientific responsibility.

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Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Cited by 176 publications

References 71 publications

A plea to reduce or replace fetal bovine serum in cell culture media

A plea to reduce or replace fetal bovine serum in cell culture media

Fetal bovine serum (FBS): Past – present – future

Propostas pedagógicas substitutivas ao uso de animais no ensino superior: uma revisão

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