A plea to reduce or replace fetal bovine serum in cell culture media

Gstraunthaler, Gerhard; Lindl, Toni; Valk, Jan van der

doi:10.1007/s10616-013-9633-8

Cited by 182 publications

(159 citation statements)

References 15 publications

Supporting

Mentioning

145

Contrasting

Unclassified

Order By: Relevance

“…According to data obtained from Jochems et al [12], cardiac puncture of the fetus begins between five and 30 minutes after the death of the dam and the bleeding procedure takes between two and five minutes. Another concern when it comes to ethical questions is the fact that FCS is mostly produced outside of the European Union; specifically, the exact origin of commercially available FCS often remains vague (e.g., origin "South America") [12,[39][40][41], making it almost impossible to trace specific FCS products.…”

Section: Discussionmentioning

confidence: 99%

Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells

Karadjian

Senger

Essers

et al. 2020

Cells

View full text Add to dashboard Cite

Fetal calf serum (FCS) is frequently used as a growth factor and protein source in bone-marrow-derived mesenchymal stromal cell (BMSC) culture media, although it is a xenogenic product presenting multiple disadvantages including but not limited to ethical concerns. A promising alternative for FCS is human platelet lysate (hPL), which is produced out of human platelet concentrates and happens to be a stable and reliable protein source. In this study, we investigated the influence of hPL in an expansion medium (ESM) and an osteogenic differentiation medium (ODM) on the proliferation and osteogenic differentiation capacity of human BMSC. Therefore, we assessed population doublings during cell expansion, performed alizarin red staining to evaluate the calcium content in the extracellular matrix and determined the activity of alkaline phosphatase (ALP) as osteogenic differentiation correlates. The proliferation rate of BMSC cultured in ESM supplemented with hPL exceeded the proliferation rate of BMSC cultured in the presence of FCS. Furthermore, the calcium content and ALP activity was significantly higher in samples incubated in hPL-supplemented ODM, especially in the early phases of differentiation. Our results show that hPL can replace FCS as a protein supplier in cell culture media and does not negatively affect the osteogenic differentiation capacity of BMSC.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells

Karadjian

Senger

Essers

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…Hydrophobic Wnt proteins require a carrier for biological activity [41] and in most experimental settings serum or detergents can suffice. These reagents, however, are contraindicated for use in humans [56]; consequently, we engineered a liposomal formulation of WNT3A (L-WNT3A) that stabilized the protein [57,58] then evaluated how the physical characteristic of the lipids affected L-WNT3A activity. The native WNT3A protein denatures within minutes at 37 C but at 23 C is stable for 25 h [37]; consequently we performed the liposomal packaging at room temperature.…”

Section: Augmenting the Endogenous Wnt Signal In Bgm Aged Restores Itmentioning

confidence: 99%

Reengineering autologous bone grafts with the stem cell activator WNT3A

et al. 2015

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…Moreover, most researchers employed FBS (known to be associated with ethical concerns and risk of xeno infections) in their protocol . Recently a well‐defined xeno‐ and serum‐free DMEM/F‐12‐based E8 medium containing only seven essential components, as opposed to FBS which contains approximately 1,800 proteins and >4,000 metabolites, was developed . When truncated vitronectin (VTN‐N) is used as an attachment matrix in conjunction with E8 medium, induced pluripotent stem cells (iPSCs) have been demonstrated to be able to maintain their tri‐lineage differentiation ability for >50 passages without any signs of karyotypic abnormalities .…”

Section: Discussionmentioning

confidence: 99%

“…Supplementation with serum [most commonly fetal bovine serum (FBS)] in cell culture may also raise the risk of batch‐to‐batch variation – this influences cell growth and can cause variability in osteogenic differentiation . The use of serum‐free and xeno‐free media ensures that the possible risks and adverse effects of animal serum are minimized with respect to future clinical applications .…”

mentioning

confidence: 99%

See 1 more Smart Citation

Localization and characterization of human palatal periosteum stem cells in serum‐free, xeno‐free medium for clinical use

Naung

Duncan

Silva

et al. 2019

European J Oral Sciences

View full text Add to dashboard Cite

Harvesting, expanding, and re‐implanting osteogenic mesenchymal stem cells (MSCs) avoids the donor‐site morbidity associated with autogenous grafting from bone marrow. Mesenchymal stem cells sourced from the palatal periosteum could be an alternative to isolation of such cells using bone marrow aspiration procedures. For safe use in human therapy, MSCs should be expanded in culture medium that is free from animal or human‐derived serum. In this study we localized, quantified, and characterized MSCs from palatal periosteum cultured in serum‐free, xeno‐free Essential 8 medium. A portion of the palatal periosteum tissues from three patients were dual‐immunostained with MSC‐specific markers (CD105, CD90, and CD73). The remaining portions were expanded in culture, and the isolated MSCs were analyzed using flow cytometry and tri‐lineage differentiation. Palatal periosteum sections were found to contain CD105‐, CD90‐, and CD73‐positive cells. The cultured cells were 73.0 ± 6.7% (mean ± SD) positive for all three MSC‐specific markers and were without hematopoietic stem cell (HSC) markers 0.5 ± 0.3% (mean ± SD). Tri‐lineage differentiation analysis confirmed that palatal periosteum cells could become adipoblasts, chondroblasts, and osteoblasts. The results demonstrate that palatal‐derived MSCs could be detected in situ within small niches, and when expanded in serum‐free, xeno‐free medium represent a viable source of MSCs for clinical use.

show abstract

A plea to reduce or replace fetal bovine serum in cell culture media

Cited by 182 publications

References 15 publications

Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells

Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells

Reengineering autologous bone grafts with the stem cell activator WNT3A

Localization and characterization of human palatal periosteum stem cells in serum‐free, xeno‐free medium for clinical use

Contact Info

Product

Resources

About