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“…According to data obtained from Jochems et al [12], cardiac puncture of the fetus begins between five and 30 minutes after the death of the dam and the bleeding procedure takes between two and five minutes. Another concern when it comes to ethical questions is the fact that FCS is mostly produced outside of the European Union; specifically, the exact origin of commercially available FCS often remains vague (e.g., origin "South America") [12,[39][40][41], making it almost impossible to trace specific FCS products.…”
Section: Discussionmentioning
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“…According to data obtained from Jochems et al [12], cardiac puncture of the fetus begins between five and 30 minutes after the death of the dam and the bleeding procedure takes between two and five minutes. Another concern when it comes to ethical questions is the fact that FCS is mostly produced outside of the European Union; specifically, the exact origin of commercially available FCS often remains vague (e.g., origin "South America") [12,[39][40][41], making it almost impossible to trace specific FCS products.…”
Section: Discussionmentioning
“…Hydrophobic Wnt proteins require a carrier for biological activity [41] and in most experimental settings serum or detergents can suffice. These reagents, however, are contraindicated for use in humans [56]; consequently, we engineered a liposomal formulation of WNT3A (L-WNT3A) that stabilized the protein [57,58] then evaluated how the physical characteristic of the lipids affected L-WNT3A activity. The native WNT3A protein denatures within minutes at 37 C but at 23 C is stable for 25 h [37]; consequently we performed the liposomal packaging at room temperature.…”
Section: Augmenting the Endogenous Wnt Signal In Bgm Aged Restores Itmentioning
“…Moreover, most researchers employed FBS (known to be associated with ethical concerns and risk of xeno infections) in their protocol . Recently a well‐defined xeno‐ and serum‐free DMEM/F‐12‐based E8 medium containing only seven essential components, as opposed to FBS which contains approximately 1,800 proteins and >4,000 metabolites, was developed .…”
Section: Discussionmentioning
“…Moreover, most researchers employed FBS (known to be associated with ethical concerns and risk of xeno infections) in their protocol . Recently a well‐defined xeno‐ and serum‐free DMEM/F‐12‐based E8 medium containing only seven essential components, as opposed to FBS which contains approximately 1,800 proteins and >4,000 metabolites, was developed . When truncated vitronectin (VTN‐N) is used as an attachment matrix in conjunction with E8 medium, induced pluripotent stem cells (iPSCs) have been demonstrated to be able to maintain their tri‐lineage differentiation ability for >50 passages without any signs of karyotypic abnormalities .…”
Section: Discussionmentioning
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