Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks

Sakai, Shinji; Kotani, Takashi; Harada, Ryohei; Goto, Ryota; Morita, Takahiro; Bouissil, Soukaina; Dubessay, Pascal; Pierre, Guillaume; Michaud, Philippe; Boutachfaiti, Redouan El; Nakahata, Masaki; Kojima, Masaru; Petit, Elsa; Delattre, Cédric

doi:10.1016/j.carbpol.2021.118820

Cited by 17 publications

(22 citation statements)

References 32 publications

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“…Human hepatoma cells and mouse fibroblasts bounded in the printed extrusion-based 3D model and exhibited an increased viability of about 95% on the next day of printing, which remained stable for 11 days. Thus, the study suggested that phenol-grafted polyglucuronic acid can be used in the field of tissue engineering, particularly as an ink component of extrusion-based 3D bioprinting [29] (Figure 3). printed by extrusion-based 3D bioprinting, which prints an object with a gradient of stiffness and cell concentration.…”

Section: Extrusion-based Printingmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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Section: Extrusion-based Printingmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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“…A study has been conducted using phenol-grafted polyglucuronic acid (PGU) as bioinks for 3D bioprinting [ 83 ]. The ability of the hydrogel to be used in cell culture was analyzed using mouse fibroblast and human hepatoma cells.…”

Section: 3d Bioprinting Applicationmentioning

confidence: 99%

Recent Advances in 3D Bioprinting: A Review of Cellulose-Based Biomaterials Ink

et al. 2022

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Cellulose-based biodegradable hydrogel proves to be excellently suitable for the medical and water treatment industry based on the expressed properties such as its flexible structure and broad compatibility. Moreover, their potential to provide excellent waste management from the unutilized plant has triggered further study on the advanced biomaterial applications. To extend the use of cellulose-based hydrogel, additive manufacturing is a suitable technique for hydrogel fabrication in complex designs. Cellulose-based biomaterial ink used in 3D bioprinting can be further used for tissue engineering, drug delivery, protein study, microalgae, bacteria, and cell immobilization. This review includes a discussion on the techniques available for additive manufacturing, bio-based material, and the formation of a cellulose-based hydrogel.

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“…Polysaccharides-based natural polymers have functional and structural diversity and have promising potential as tissue engineering scaffolds [ 10 , 11 , 12 , 13 ]. In tissue engineering, alginate (Alg) is the most commonly utilized natural polysaccharide for hydrogelation [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Microbial EPSs such as bacterial Alg are produced by a variety of bacteria and fungi and are involved in a variety of physiological processes, including biofilm construction, nutrition acquisition, stress resistance, and antimicrobial resistance [ 39 , 40 ]. In this particular context, a polyglucuronic acid called glucuronan (PGU), as an EPS produced by the Sinorhizobium meliloti M5N1CS strain, a Gram-negative α-proteobacterium capable of fixing atmospheric nitrogen, described a high molecular weight anionic homopolysaccharide made up of →(4)- β -D-GlcpA-(1)→residue partially O -acetylated at the C-3 and/or the C-2 position [ 10 , 39 , 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

“…This bacterial EPS is a biocompatible, non-cytotoxic, and bioactive biopolymer used in cosmetics and pharmaceuticals as a hydrating, thickening, and gelling agent. PGU was recently considered for tissue engineering applications as a very promising polysaccharides-based biomaterial used in functional tissue fabrication [ 10 , 44 ] and could be efficiently used and investigated for new applications in tissue engineering, as already done with alginate [ 44 ], and can be utilized as an alternative for pectin and alginate in the cosmetic and pharmaceutical industries [ 42 , 45 , 46 , 47 ]. Furthermore, the immunostimulating properties of PGU and oligo-PGU were mentionned in a French patent (FR2781673), and they have been demonstrated on human blood monocytes to be better productions of Il-6 and TNF-α cytokines and equivalent productions of Il-1 cytokine in comparison with alginate.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering

et al. 2022

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3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue engineering scaffold. The CNFs were obtained from electrospun polyacrylonitrile nanofibers through heat treatment. The fabricated CNFs were incorporated into a PGU/Alg polymeric solution, which was physically cross-linked using CaCl2 solution. The fabricated nanocomposites were characterized to evaluate the internal structure, porosity, swelling kinetics, hemocompatibility, and cytocompatibility. The characterizations indicated that the nanocomposites have a porous structure with interconnected pores architecture, proper water absorption, and retention characteristics. The in vitro studies revealed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability assessment showed that the nanocomposites were biocompatible and supported bone cell growth. These results indicated that the fabricated bacterial PGU/Alg/CNFs hydrogel nanocomposite exhibited appropriate properties and can be considered a new biomaterial for bone tissue engineering scaffolds.

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Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks

Cited by 17 publications

References 32 publications

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Recent Advances in 3D Bioprinting: A Review of Cellulose-Based Biomaterials Ink

Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering

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