Development of PDA/Phospholipids/Lysine vesicles to detect pathogenic bacteria

Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging

“…E. coli ATCC 10536 and S. aureus ATCC 25923 were used as gram-negative and gram-positive control, respectively, to ensure that the color change did not occur due to the presence of other pathogens. According to Oliveira et al [23] and Pires et al [1], E. coli and S. aureus may change color in less than 48 h when using Lysine and N-[(2-teradecanamide)-ethyl]-ribonamide as receptors or without receptor incorporation. This means that phage PVP-SE1 incorporation cause specific interaction between Salmonella serovar in PDA vesicles.…”

Stability and sensitivity of polydiacetylene vesicles to detect Salmonella

“…This phenomenon is called Thermochromism and it could affect the PDA particle size to some extent. On the other hand, the temperature could also provide the energy necessary for changes in the PDA chain to become more stable at 32.5 • C and consequently causes changes in optical absorption and the color transition [2][3][4][5]7,8,19].…”

“…Among the conjugated polymers reported to date, PDAs are one of particular interest because they can change color from blue to red in response to external stimuli and can be auto-arranged in liposome assembly [2][3][4][5][6][7][8]; these characteristics could be manipulated by lipid insertion modifying biointerfacial interactions and it could be utilized in sensing technologies to improve biosensors [9,10]. The polydiacety…”

“…PDAs modified with specific receptors, for example antibodies that have been used to detect bacteria or a virus. The color transition of these PDA materials upon exposure to the stimuli can be observed by the naked eye making it simple to utilize them [7,8,14].…”

Polydiacetylene sensor interaction with food sanitizers and surfactants

“…Since then, great progress has been made in developing PDA vesicle based sensors. Sensors have also been developed that can rapidly detect cations (Kolusheva, Shahal, & Jelinek, 2000), proteins (Jung, Kim, Park, & Soh, 2010), melamine (Lee, Jeong, & Kim, 2011), bacteria toxins (Ma & Cheng, 2005), lipopolysaccharides (Rangin & Basu, 2004;Wu, Zawistowski, Ehrmann, Yi, & Schmuck, 2011) and bacteria (de Oliveira et al, 2013;Nagy et al, 2008;Silbert et al, 2006).…”