Phage PVP-SE1 as Tool Recognition in Polydiacetylene to Produce Intelligent Packaging

Abstract

Polydiacetylene (PDA) compound has unique chromatic properties when embedded in methylcellulose base. Therefore, this could be used as intelligent packaging. PDA exhibits blue-to-red color transition under certain stimuli, which was caused by alterations in PDA conjugation length backbone. For a specific color modification, bio-recognition tools were covalently linked with PDA. In this work, a broad-spectrum virulent phage (PVP-SE1) was used as a biorecognition element to detect Salmonella cells due to PDA color change. A series of experiments were performed to monitor colorimetric response. Consequently, chromatic immunoassay could be significant in increasing potential applications of PDA intelligent packaging.

Biomimetic System Characterization: pH-Induced Chromatic Transition and Nanostructural Transformation of Polydiacetyle and Dimyristoylphosphatidylcholine Vesicles Under pH Variation Using Dynamic Light Scattering (DLS) Technique

“…The values of the colorimetric response (CR) were 18%, 27% and 40% for pH 9.1; 10.0 and 12.2, respectively ( Figure 3). According Oliveira et al [21], RC of 15% is enough to visualize the color change from blue to red. These results suggest that the conditions of exposure of vesicles to pH values above 9.0 may change the colorimetric properties of the vesicles, while the conditions with pH values lower than 4.0 may promote destabilization of the vesicles.…”