Development of OPS vitrified pig blastocysts: Effects of size of the collected blastocysts, cryoprotectant concentration used for vitrification and number of blastocysts transferred

Berthelot, F.; Venturi, Éric; Cognié, Juliette; Furstoss, V.; Martinat-Botté, F.

doi:10.1016/j.theriogenology.2007.04.050

Cited by 21 publications

(15 citation statements)

References 18 publications

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“…Pig blastocysts at later stages are known to be more cryotolerant than early cleavage stage embryos [45][46][47][48]. In this study, however, morulae and early blastocysts were vitrified, and the viability of the embryos was similar to or higher than in previous studies [49,50], indicating the effectiveness of the HFV method.…”

Section: Matsunari Et Al 606supporting

confidence: 39%

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Matsunari

Maehara

Nakano

et al. 2012

Journal of Reproduction and Development

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Abstract. Current embryo vitrification methods with proven efficacy are based on the minimum volume cooling (MVC) concept by which embryos are vitrified and rewarmed ultrarapidly in a very small amount of cryopreserving solution to ensure the high viability of the embryos. However, these methods are not suitable for simultaneously vitrifying a large number of embryos. Here, we describe a novel vitrification method based on use of a hollow fiber device, which can easily hold as many as 40 mouse or 20 porcine embryos in less than 0.1 µl of solution. Survival rates of up to 100% were obtained for mouse embryos vitrified in the presence of 15% DMSO, 15% ethylene glycol and 0.5 M sucrose using the hollow fiber vitrification (HFV) method, regardless of the developmental stage of the embryos (1-cell, 2-cell, morula or blastocyst; n = 50/ group). The HFV method was also proven to be effective for vitrifying porcine in vitro-and in vivo-derived embryos that are known to be highly cryosensitive. For porcine embryos, the blastocyst formation rate of in vitro maturation (IVM)-derived parthenogenetic morulae after vitrification (48/65, 73.8%) did not decrease significantly compared with non-vitrified embryos (59/65, 90.8%). Transfer of 72 in vivo-derived embryos vitrified at the morula/early blastocyst stages to 3 recipients gave rise to 29 (40.3%) piglets. These data demonstrate that the HFV method enables simultaneous vitrification of multiple embryos while still adhering to the MVC concept, and this new method is very effective for cryopreserving embryos of mice and pigs. Key words: Cryopreservation, Hollow fiber, Mouse embryos, Pig embryos, Vitrification (J. Reprod. Dev. 58: [599][600][601][602][603][604][605][606][607][608] 2012) E mbryo cryopreservation is widely and routinely used both for research in reproductive biology and for the development of practical applications in animal industries and human reproductive medicine. The cryopreservation of oocytes and embryos is essential for the long-term preservation of valuable genetic resources in experimental and livestock animals [1][2][3][4][5], and the cryopreservation of embryos improves pregnancy rates in assisted reproductive technology applications [6][7][8]. Various protocols have been developed for the cryopreservation of animal embryos, and many are used in research and clinical applications [1,5,9].In recent years, embryo vitrification has been applied to an increasing number of animal species, and high post-cryopreservation embryo viability has been achieved [10,11]. The basic concept of vitrification is as follows: a solution containing a high concentration of a cryoprotective agent (CPA) is rapidly cooled, causing it to transform from the liquid phase to the solid phase without forming ice crystals. As a result, cells suspended in the solution are preserved in an ultra-low temperature glassy (amorphous) material [10,12]. To create this glassy state, a solution containing a high concentration of CPA (normally 4-6 M) needs to be cooled ultrarapidly. Howeve...

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Section: Matsunari Et Al 606supporting

confidence: 39%

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Matsunari

Maehara

Nakano

et al. 2012

Journal of Reproduction and Development

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“…Based on differential cell staining of fresh blastocysts, we inferred that ICSI-derived expanding blastocysts and their IVF-derived counterparts were of comparable quality (in total cell number and the ICM ratio); therefore, differences in embryo quality before vitrification apparently did not account for the higher sensitivity of ICSI-derived expanding blastocysts to MVC vitrification. The relationship between the size of blastocysts and their cryotolerance has been investigated; larger blastocysts tolerated cryopreservation better than the smaller ones [9,25,29,30], with the exception of one report in cattle [3]. For speciesspecific reasons, there was opposite size-dependent cryotolerance in human [31] and horse [32] blastocysts.…”

Section: Discussionmentioning

confidence: 99%

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

et al. 2010

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“…In contrast to this finding, surgical ET studies evaluating the pregnancy rates and the number of viable fetuses on Day 30 of gestation indicated that 20 vitrified embryos might be the optimal number of embryos for transfer to each recipient and that little benefit is gained from the transfer of more embryos [59]. Although there were important methodological differences between the two studies (e.g., ET procedure, breed of recipients, age of recipients, and method of vitrification), previous evidence supports our results.…”

Section: Recipients (N) Embryos Transferred (N) Embryo Transfer Methomentioning

confidence: 93%

Recent advances toward the practical application of embryo transfer in pigs

et al. 2016

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Development of OPS vitrified pig blastocysts: Effects of size of the collected blastocysts, cryoprotectant concentration used for vitrification and number of blastocysts transferred

Cited by 21 publications

References 18 publications

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device

Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

Recent advances toward the practical application of embryo transfer in pigs

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