Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

Abdalla, Hany; Shimoda, Masafumi; Hara, Hiromasa; Morita, Hiroshi; Kuwayama, Masashige; Hirabayashi, Masumi; Hochi, Shinichi

doi:10.1016/j.theriogenology.2010.04.033

Cited by 18 publications

(12 citation statements)

References 36 publications

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“…Contrary to this, in our experiment there was no significant difference in survival and hatching rates found between day 7 and 8 expanded blastocysts at 24 and 48 h post warming. These results are in agreement with Abdalla et al (2010). Moreover, in our case the survival rate of day 7 and 8 expanded blastocysts shows no significant decline during culture unlike tendency shown by day 7 and 8 blastocysts.…”

Section: Discussionsupporting

confidence: 93%

“…The survival rate (90%) in the experimental group at 24 h post warming was comparable to those obtained after group vitrification of IVP bovine embryos using hollow fibers (Beck et al, 2013) and various open-type carriers (Abdalla et al, 2010;Kim et al, 2012). The highest survival rates of the bovine embryos (100%) were obtained using McGill Cryoleaf (Huang et al, 2007) and Cryotech (Gutnisky et al, 2013).…”

Section: Discussionsupporting

confidence: 53%

“…Thus, our preliminary data obtained using Cryotop carrier showed leveling of the difference in survival rates of day 7 IVP bovine blastocysts (17/18 embryos) and expanded blastocysts (15/15 embryos). No significant difference in the survival rates was also found between day 7 or 8 expanding (85 and 84%) and fully expanded blastocysts (88 and 89%) vitrified using Cryotop (Abdalla et al (2010) and maximum survival rates of the mixed population of day 7 bovine blastocysts (100%) were obtained using close analogues of this carrier (Huang et al, 2007;Gutnisky et al, 2013).…”

Section: Discussionmentioning

confidence: 97%

“…For example, the division of the embryos according to their developmental stage is highly subjective (Dinnyes et al, 1999;Gomez et al, 2008;Abdalla et al, 2010). However, one of the major factors that may contribute to the differences in the survival rates of vitrified bovine embryos of different developmental stage and age seems to be the choice of the either surface type carriers based on MVC principle (Huang et al, 2007;Abdalla et al, 2010;Gutnisky et al, 2013) or tubing carriers (Dinnyes et al, 1999;Gomez et al, 2008;Rios et al, 2010). Thus, our preliminary data obtained using Cryotop carrier showed leveling of the difference in survival rates of day 7 IVP bovine blastocysts (17/18 embryos) and expanded blastocysts (15/15 embryos).…”

Section: Discussionmentioning

confidence: 99%

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A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

et al. 2017

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A new vitrification device based on hollow fiber vitrification (HFV) was constructed using a glass capillary, which lead to simplified construction process and increased practicality of the device. The hollow fiber was attached to heat-pulled tip of the glass capillary using forceps. A protective sheath fitted on the capillary provided protection for the cellulose triacetate hollow fiber with loaded embryos and allowed safe storage in liquid nitrogen for long periods of time (2-12 month), transfer between tanks with liquid nitrogen and transportation within these tanks. No embryos were lost in the process. The device was tested using seven-dayold and eight-day-old IVP bovine blastocysts and expanded blastocysts as a model. Obtained survival (90% at 24 h post warming) and hatching rates (62% at 72 h post warming) of day 7 blastocysts and expanded blastocysts were comparable to those gained using various vitrification carriers. Vitrified embryos did not show an increase in the number of cells with damaged membrane or a decrease in total cell number per embryos in comparison to their non-vitrified counterparts. Day 7 and 8 expanded blastocysts did not differ significantly in terms of survival at 24 (97.01 vs. 97.50%) and 48 h post warming (95.52 vs. 95%), but showed significantly higher survival and hatching rates than day 7 and 8 blastocysts. These results indicated that high and repeatable survival rates can be obtained by selection of IVP bovine embryos at the developmental stage of expanded blastocyst for HFV. Further modification of the method may be required to achieve high and stable results with different developmental stages of IVP bovine embryo. The vitrification device presented in the current article may contribute to wider application of HFV method in livestock production.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

et al. 2017

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“…A possible adverse effect of cryopreservation is the reduced ability of post-warming embryos to continue mitotic division (Kaidi et al, 1998), or the induction of apoptosis or necrosis in some embryonic cells (Abdalla et al, 2010). The adverse effect of vitrification/warming in this study was consistent with the commonly reported losses of these processes, which is in agreement with results reported for cows (George et al, 2006;Abdalla et al, 2010;Ha et al, 2014).…”

Section: Discussionsupporting

confidence: 91%

Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos

Reyes

Jaramillo

2016

Rev Colomb Cienc Pecu

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Facultad de Ciencias Agropecuarias, Universidad de la Salle. Carrera. 7 No. 172-85. Bogotá, Colombia. (Received: November 13, 2014; accepted: January 27, 2016) doi: 10.17533/udea.rccp.v29n2a06 Summary Background: an optimal formulation for vitrifying in vitro-produced (IVP) bovine embryos is currently unavailable. Objective: to estimate whether differences in composition of vitrification solutions may affect the viability of IVP embryos as compared to that of embryos cryopreserved by a conventional slow-freezing method. Methods: bovine IVP embryos were cryopreserved by two methods: 1) a slow controlled-rate (1.5 M ethylene glycol (EG)), or 2) vitrification by using two different vitrification and thawing/warming solutions: (1) Protocol V1: commercial vitrification Kit, and (2) Protocol V2: defined vitrification (20% EG; 20% dimethyl sulfoxide (DMSO); 20% fetal bovine serum (FBS)) and warming (20% FBS; 0.2 M sucrose) solutions. Embryo viability was recorded at 24, 48, and 72 h after thawing/warming by evaluating the number of embryos that re-expanded and developed to the hatching blastocyst stage. Results: embryo survival rate was affected by the method of cryopreservation, where the frequency of embryos that re-expanded at 24 h after thawing/warming was higher for embryos vitrified with protocols V1 and V2 (89.0%, 86.2%, respectively) compared to those cryopreserved by the slow-controlled rate method (73.6%, p<0.05). Similarly, higher percentage of embryos cryopreserved by vitrification hatched at 72 h, where protocol V2 resulted in higher percentage of hatched embryos (84.3%) compared to protocol V1 (64,0%, p<0.05), and both were higher compared to the slowcontrolled rate method (55.2%, p<0.05). Conclusions: the method of cryopreservation and composition of the vitrification solution have a direct effect on the viability of bovine IVP embryos.

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Vitrification of in vitro produced goat blastocysts: Effects of oocyte donor age and development stage

et al. 2011

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Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age

Cited by 18 publications

References 36 publications

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos

Vitrification of in vitro produced goat blastocysts: Effects of oocyte donor age and development stage

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