Recent advances toward the practical application of embryo transfer in pigs

Martı́nez, Emilio A.; Cuello, C.; Parrilla, Inmaculada; Martínez, Cristina A.; Nohalez, Alicia; Vázquez, José Luís; Vázquez, Juan M.; Roca, Jordi; Gil, M.A.

doi:10.1016/j.theriogenology.2015.06.002

Cited by 40 publications

(37 citation statements)

References 59 publications

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“…On the other hand, the quality of embryos from zygotes stored for 24 hr decreased as compared with that from fresh zygotes, even when the zygotes were stored with an adequate concentration of CGA. Previous studies indicate that suboptimal conditions for porcine short‐term embryo storage affect embryo viability and quality (Martinez et al, ). Therefore, it is important to improve the conditions that allow zygotes to retain their full viability during storage in vitro.…”

Section: Discussionmentioning

confidence: 99%

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Nguyen

Hirata

Tanihara

et al. 2019

Reprod Domestic Animals

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Contents The current study was conducted to investigate the effects of 100% foetal bovine serum (FBS) and 100% porcine follicular fluid (pFF) as a storage medium on the developmental competence of porcine zygotes stored at 25°C for 24 hr. Moreover, we evaluated the additive effects of chlorogenic acid (CGA) in the storage medium. When in vitro‐produced zygotes were stored at 25°C for 24 hr in tubes containing either tissue culture medium (TCM) 199 supplemented with 1 mg/ml bovine serum albumin (BSA), 100% of FBS or 100% of pFF, the rate of blastocyst formation was significantly higher in 100% of FBS than in BSA‐containing TCM 199. When the effects of CGA supplementation in 100% of FBS on the development of zygotes stored at 25°C for 24 hr was evaluated, more zygotes stored with 50 µM CGA developed to blastocysts compared with the other concentrations of CGA. When the formation date and quality of blastocysts derived from zygotes stored in 100% of FBS supplemented with 50 µM CGA were investigated, the highest ratio of blastocysts formation in the storage group appeared 1 day later than in the non‐stored control group. However, a higher proportion of blastocysts with apoptotic nuclei was observed in the stored group as compared to the non‐stored group. In conclusion, 100% of FBS is available for a short storage medium of porcine zygotes. The supplementation of 50 µM CGA into the storage medium improves the rates of blastocyst formation of zygotes after storage, but the quality of embryos from the stored zygotes remains to be improved.

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Section: Discussionmentioning

confidence: 99%

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Nguyen

Hirata

Tanihara

et al. 2019

Reprod Domestic Animals

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“…Several previous studies indicated that the insertion of the NsDU-ET catheter is non-traumatic (no bleeding during or after insertions), painless and well-tolerated by the recipients, even when the difficulty in inserting the catheter is increased (reviewed in [8,9,20].…”

Section: Discussionmentioning

confidence: 99%

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

et al. 2017

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“…Reproductive technologies, mainly artificial insemination (AI) and embryo transfer (ET), are the best means to, spreading genetic progress, improve global swine populations [2]. Although considerable progress has recently been made in long-term embryo preservation and non-surgical ET methodologies in pigs, their practical and commercial implementation are yet distant (reviewed in [3]); AI being left as sole practical tool for global pig gene dissemination.…”

Section: Introductionmentioning

confidence: 99%

Will AI in pigs become more efficient?

Roca

Parrilla

Bolarín³

et al. 2016

Theriogenology

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Artificial insemination (AI) is applied worldwide to commercially breed pigs, with fertility outcomes similar to those of natural mating. However, it is not fully efficient, as only liquid-stored semen is used, with a single boar inseminating about 2,000 sows yearly.Use of liquid semen, moreover, constrains international trade and slows genetic improvement. Research efforts, reviewed hereby, are underway to reverse this inefficient scenario. Special attention is paid to studies intended to decrease the number of sperm used per pregnant sow, facilitating the practical use of sexed frozen-thawed semen in swine commercial insemination programs.

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Recent advances toward the practical application of embryo transfer in pigs

Cited by 40 publications

References 59 publications

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Will AI in pigs become more efficient?

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