Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Martínez, Cristina A.; Nohalez, Alicia; Parrilla, Inmaculada; Vázquez, José Luís; Roca, Jordi; Cuello, C.; Rodriguez‐Martinez, Heriberto; Martı́nez, Emilio A.; Gil, M.A.

doi:10.1016/j.theriogenology.2016.09.009

Cited by 15 publications

(12 citation statements)

References 18 publications

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“…Embryos were retrieved on Day 6 from inseminated sows as previously reported [3]. Sedation and general anesthesia were achieved by azaperone (i.m.…”

Section: Embryo Collectionmentioning

confidence: 99%

“…Despite these important advantages, the use of ET in pigs has not reached the levels attained in other domestic animals, such as cattle. Although the main drawback for a broad application of ET in pigs comes from the necessity of using surgical methods to retrieve embryos, a lower litter size is also a determinant for its limited use [2,3]. Approximately 55% of transferred embryos die before, during, or shortly after implantation in the reproductive tract of recipients after ET, values almost 25 percentage points higher than those obtained by artificial insemination (AI) [4].…”

Section: Introductionmentioning

confidence: 99%

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Blastocyst-Bearing Sows Display a Dominant Anti-Inflammatory Cytokine Profile Compared to Cyclic Sows at Day 6 of the Cycle

Parrilla

Martínez

Cambra

et al. 2020

Animals

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In the context of porcine embryo transfer (ET) technology, understanding the tightly regulated local uterine immune environment is crucial to achieve an adequate interaction between the transferred embryos and the receiving endometrium. However, information is limited on the uterine immune status of cyclic-recipient sows when receiving embryos during ET. The present study postulated that the anti- and proinflammatory cytokine profile 6 days after the onset of estrus differs between endometria from uninseminated cyclic sows and blastocyst-bearing sows. On Day 6 of the cycle, endometrial explants were collected from sows inseminated or not inseminated during the postweaning estrus and cultured for 22 h. The culture medium was then analyzed for the contents of a total of 16 cytokines using Luminex MAP® technology. The results showed important differences in the endometrial production of most cytokines between the sow categories, with a predominant anti-inflammatory environment displayed by the blastocyst-bearing endometria. These findings suggest that sperm, seminal plasma (SP) and/or early embryos modify the uterine environment by inducing an immune-tolerant cytokine profile already visible at Day 6. Whether the SP or some of its active components may help to develop strategies to maximize the reproductive performance of recipients after ET needs further investigation.

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“…Embryos were retrieved on Day 6 from inseminated sows as previously reported [3]. Sedation and general anesthesia were achieved by azaperone (i.m.…”

Section: Embryo Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Blastocyst-Bearing Sows Display a Dominant Anti-Inflammatory Cytokine Profile Compared to Cyclic Sows at Day 6 of the Cycle

Parrilla

Martínez

Cambra

et al. 2020

Animals

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“…A usual concern of genetic and production companies is the impact that surgical embryo collection and NsDU‐ET procedures have on the future reproductive performance of the donors and recipients, respectively, which previous studies never examined. Recently, we evaluated the reproductive performance of donors and recipients after AI subsequent to surgical embryo collection and NsDU‐ET procedures, respectively (Martinez et al., ). We found that while the farrowing rate of donors was not affected by surgery, prolificacy was deeply reduced (two piglets less).…”

Section: Factors Affecting the Et Technologymentioning

confidence: 99%

Achievements and future perspectives of embryo transfer technology in pigs

Martı́nez

Martínez

Cambra

et al. 2019

Reprod Domestic Animals

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Commercial embryo transfer (ET) has unprecedented productive and economic implications for the pig sector. However, pig ET has been considered utopian for decades mainly because of the requirements of surgical techniques for embryo collection and embryo deposition into recipients, alongside challenges to preserve embryos. This situation has drastically changed in the last decade since the current technology allows non-surgical ET and short-and long-term embryo preservation. Here, we provide a brief review of the improvements in porcine ET achieved by our laboratory in the past 20 years. This review includes several aspects of non-surgical ET technology and different issues affecting ET programmes and embryo preservation systems.The future perspectives of ET technology are also considered. We will refer only to embryos produced in vivo since they are the only type of embryos with possible short-term use in pig production. K E Y W O R D Sembryo storage, embryo transfer, porcine, vitrification | 5 MARTINEZ ET Al.

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“…For embryo recovery, the reproductive tract was exposed via mid-line incision, corpora lutea were counted on the ovaries, and the embryos were collected as previously reported (19). Briefly, a small incision in the uterine wall 30-40 cm below the uterotubal junction was performed with a blunt Adson forcep, and then, a glass cannula was inserted through the incision.…”

Section: Embryo and Tissue Collectionmentioning

confidence: 99%

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

et al. 2019

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Results: Embryo viability was equal between the groups (∼93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others.Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.

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Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows

Abstract: Surgical embryo collection but not nonsurgical embryo transfer compromises postintervention prolificacy in sows, Theriogenology, 2017. 87, pp.316-320. http

Cited by 15 publications

References 18 publications

Blastocyst-Bearing Sows Display a Dominant Anti-Inflammatory Cytokine Profile Compared to Cyclic Sows at Day 6 of the Cycle

Blastocyst-Bearing Sows Display a Dominant Anti-Inflammatory Cytokine Profile Compared to Cyclic Sows at Day 6 of the Cycle

Achievements and future perspectives of embryo transfer technology in pigs

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

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