Development of one-step reverse transcription loop-mediated isothermal amplification for norovirus detection in oysters

Jeon, Su Been; Seo, Dong Joo; Oh, Hyejin; Kingsley, David H.; Choi, Changsun

doi:10.1016/j.foodcont.2016.10.005

Cited by 32 publications

(23 citation statements)

References 51 publications

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“…In addition, Moore and Jaykus [108] developed a rapid (<30 min) RPA assay for the detection of HuNoV RNA from outbreak samples, with a LOD of 3.4 log 10 gc. Jeon et al [109] developed a one-step RT-LAMP assay for the detection of GI and GII HuNoV and found that one step RT-LAMP was 10~100 times more sensitive than real time RT-PCR. A colorimetric RT-LAMP assay with a metal ion-binding indicator dye (hydroxynaphthol blue dye) was reported to have a sensitivity of 10 3 gc per reaction and a detection rate of 94.83% in detection of GII NoV [110].…”

Section: Isothermal Amplification Methodsmentioning

confidence: 99%

A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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As the leading cause of acute gastroenteritis worldwide, human noroviruses (HuNoVs) have caused around 685 million cases of infection and nearly $60 billion in losses every year. Despite their highly contagious nature, an effective vaccine for HuNoVs has yet to become commercially available. Therefore, rapid detection and subtyping of noroviruses is crucial for preventing viral spread. Over the past half century, there has been monumental progress in the development of techniques for the detection and analysis of noroviruses. However, currently no rapid, portable assays are available to detect and subtype infectious HuNoVs. The purpose of this review is to survey and present different analytical techniques for the detection and characterization of noroviruses.

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Section: Isothermal Amplification Methodsmentioning

confidence: 99%

A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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“…Table 2. Comparison between PCR and loop-mediated isothermal amplification (LAMP) reactions [37,[40][41][42].…”

Section: A Potential Candidate For Rapid Detection Of Sars-cov-2: Loomentioning

confidence: 99%

“…For virus detection, for example, influenza [40] or human norovirus, LAMP assay offers one-step detection [41]. Sample preparation steps are simplified.…”

Section: Always Requires Sample Concentration and Preparation (Time-cmentioning

confidence: 99%

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2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics

2020

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“…As a response to the urgent demand for more accurate and reliable detection, here the gene segments of NoV GII was specifically employed as the model target [39]. In spite of their high sensitivity, earlier reports like electron microscopy [40,41], immunology-based detection (radioimmunoassay (RIA) [42,43], enzyme-linked immunosorbent assays (ELISA) [44,45]) and nucleic acids biomarkers detection based on gene amplification techniques (PCR [46,47], nucleic acid sequence-based amplification (NASBA) [48,49], LAMP [50,51]) always lack of specificity and false positive results can easily arise. Herein, the powerful exponential amplification of LAMP results in ultrasensitive NoV gene detection with a detection limit of 30 copies, while the HCR gives rise to very good specificity and reliable signal changes in all kinds of readouts.…”

Section: Introductionmentioning

confidence: 99%