Development of novel techniques to extract phenolic compounds from Romanian cultivars of Prunus domestica L. and their biological properties

Mocan, Andrei; Diuzheva, Alina; Carradori, Simone; Andruch, Vasiľ; Massafra, Chiara; Moldovan, Cadmiel; Sisea, Cristian Radu; Petzer, Jacobus P.; Petzer, Anél; Zara, Susi; Marconi, Guya Diletta; Zengin, Gökhan; Crișan, Gianina; Locatelli, Marcello

doi:10.1016/j.fct.2018.04.045

Cited by 41 publications

(31 citation statements)

References 45 publications

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“…The highest TFC value was 9.76 ± 0.23 mg QE/g dw walnut septum (run order 14, Table 3 ), approximately 10 times lower than total extractable phenolics. In one recent study the total flavonoids content of the walnut leaf extract ranged from 5.52 to 28.48 mg QE/g [ 57 ], while Mocan et al (2018), researching Prunus domestica leaves, found TFC values between 36.60 ± 2.90 and 60.32 ± 4.12 mg QE/g leaf extract [ 59 ]. However, an objective comparison between the results is quite difficult, due to different matrixes and extraction protocols.…”

Section: Resultsmentioning

confidence: 99%

Process Optimization for Improved Phenolic Compounds Recovery from Walnut (Juglans regia L.) Septum: Phytochemical Profile and Biological Activities

et al. 2018

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Plant by-products can be valuable sources of polyphenol bioactive compounds. Walnut (Juglans regia L.) is a very important tree nut rich in biologically active molecules, but its septum was scarcely researched. Experimental data indicated a hypoglycemic effect of septum extracts, with almost no details about its phytochemical composition. The main objectives of this study were: (1) to obtain walnut septum (WS) extracts with high content in bioactive compounds and antioxidant activity based on an original experimental design; (2) characterization of the phytochemical profile of the WS extracts using HPLC-MS/MS; (3) evaluation of the biological potential of the richest polyphenolic WS extract. The variables of the experimental design were: extraction method (maceration and Ultra-Turrax extraction), temperature, solvent (acetone and ethanol), and percentage of water in the solvent. The first quantifiable responses were: total phenolic content, total flavonoid content, condensed tannins, and ABTS antioxidant capacity. The phytochemical profile of lyophilized extracts obtained by Ultra-Turrax extraction (UTE), the most efficient method, was further determined by HPLC-MS/MS analysis of individual polyphenolic and phytosterols compounds. It is the first study to assay the detailed composition of WS in hydrophilic and lipophilic compounds. The biological potential of the richest polyphenolic WS extract was also evaluated by FRAP and DPPH antioxidant capacity and the inhibition of tyrosinase, an enzyme involved in the browning in fruits and vegetables, skin wrinkles and aging. Conclusion: The phytochemical profile of the analyzed extracts proves that WS can be a valuable source of biologically active compounds (polyphenols) for food and/or pharmaceutical industry and warrant the continuation of current research in further evaluating its bioactive potential.

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Section: Resultsmentioning

confidence: 99%

Process Optimization for Improved Phenolic Compounds Recovery from Walnut (Juglans regia L.) Septum: Phytochemical Profile and Biological Activities

et al. 2018

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“…In addition to hesperidin, other bioactive chemical markers are all phenolic acids. Phenolic acids exist widely in nature and have many pharmacological effects [ 21 , 22 ]. Stalmach A. and other researchers have shown that chlorogenic acid is absorbed into the blood in only about 30% of the small intestine, while 70% are absorbed by intestinal flora after metabolism [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the previous study, our laboratory found that 35% ethanol extract of ZZX had a stable anti-anxiety effect [ 19 ]. This part of the extract contains a lot of phenolic acids, which exist widely in nature and have many pharmacological effects [ 21 , 22 ]. Hence, we selected 35% ethanol extract of ZZX to study the fingerprint-efficacy relationship, and to find the bioactive chemical markers of its anti-anxiety activity.…”

Section: Introductionmentioning

confidence: 99%

Identification of Bioactive Chemical Markers in Zhi zhu xiang Improving Anxiety in Rat by Fingerprint-Efficacy Study

Wang

Ding

et al. 2018

Molecules

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Zhi zhu xiang (ZZX for short) is the root and rhizome of Valeriana jatamansi Jones, which is a Traditional Chinese Medicine (TCM) used to treat various mood disorders for more than 2000 years, especially anxiety. The aim of the present work was to identify the bioactive chemical markers in Zhi zhu xiang improving anxiety in rats by a fingerprint-efficacy study. More specifically, the chemical fingerprint of ZZX samples collected from 10 different regions was determined by High Performance Liquid Chromatography (HPLC) and the similarity analyses were calculated based on 10 common characteristic peaks. The anti-anxiety effect of ZZX on empty bottle stimulated rats was examined through the Open Field Test (OFT) and the Elevated Plus Maze Test (EPM). Then we measured the concentration of CRF, ACTH, and CORT in rat’s plasma by the enzyme-linked immune sorbent assay (ELISA) kit, while the concentration of monoamine and metabolites (NE, DA, DOPAC, HVA, 5-HT, 5-HIAA) in the rat’s cerebral cortex and hippocampus was analysed by HPLC coupled with an Electrochemical Detector. At last, the fingerprint-efficacy study between chemical fingerprint and anti-anxiety effect of ZZX was accomplished by partial least squares regression (PLSR). As a result, we screened out four compounds (hesperidin, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C) as the bioactive chemical markers for the anti-anxiety effect of ZZX. The fingerprint-efficacy study we established might provide a feasible way and some elicitation for the identification of the bioactive chemical markers for TCM.

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“…Qualitative and qualitative analysis of phenolic compounds was performed according to a previously reported method [17], using a chromatographic system consisted of HPLC Waters liquid chromatograph instrument (model 600 solvent pump, 2996 PDA; Waters Spa, Milford, MA, USA). Mobile phase was directly degassed online by using a Biotech 4CH DEGASI Compact (Onsala, Sweden).…”

Section: Hplc-pda Analysis Of Phenolic Compoundsmentioning

confidence: 99%

Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur

et al. 2020

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Even though Salvia genus is one of the most known and studied taxa of Lamiaceae family, the knowledge regarding the chemical composition and health-related benefits of some locally used Salvia species (mostly endemic) is still scarce. In this regard, the present work aims to evaluate the chemical profile and potential bioactivities of 70% (v/v) ethanolic extracts obtained from the less-studied S. transsylvanica and S. glutinosa in comparison with S. officinalis. HPLC-PDA analysis revealed the presence of rutin and catechin as the main compounds in the extracts of the three studied species (using the employed HPLC method), whereas the presence of naringenin was highlighted only in S. glutinosa extract. Chlorogenic acid, rutin and quercetin were identified and quantified for the first time in S. transsylvanica extracts. The in vitro antioxidant capacity of each extract was tested through complementary methods (phosphomolybdenum assay, DPPH, ABTS, CUPRAC and FRAP assays), and correlated with the presence of phenolics (especially flavonoids) in high amounts. The neuroprotective and antidiabetic abilities of S. officinalis (the most active as AChE, BChE and α-glucosidase inhibitor), S. glutinosa (the most active as α-amylase inhibitor) and S. transsylvanica were also studied. For each extract it was determined the antimicrobial, antifungal and cytotoxic effects using in vitro assays. The obtained results confirm the potential of S. transsylvanica and S. glutinosa as promising sources of bioactive compounds and as a starting point for further analyses.

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Development of novel techniques to extract phenolic compounds from Romanian cultivars of Prunus domestica L. and their biological properties

Cited by 41 publications

References 45 publications

Process Optimization for Improved Phenolic Compounds Recovery from Walnut (Juglans regia L.) Septum: Phytochemical Profile and Biological Activities

Process Optimization for Improved Phenolic Compounds Recovery from Walnut (Juglans regia L.) Septum: Phytochemical Profile and Biological Activities

Identification of Bioactive Chemical Markers in Zhi zhu xiang Improving Anxiety in Rat by Fingerprint-Efficacy Study

Chemical Constituents and Biologic Activities of Sage Species: A Comparison between Salvia officinalis L., S. glutinosa L. and S. transsylvanica (Schur ex Griseb. & Schenk) Schur

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