Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples

Guo, Pengqi; Luo, Zhimin; Xu, Xinya; Zhou, Yulan; Zhang, Bilin; Chang, Ruimiao; Du, Wei; Chang, Chun; Fu, Qiang

doi:10.1016/j.foodchem.2016.09.021

Cited by 48 publications

(14 citation statements)

References 27 publications

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“…Some studies have explored all the possibilities of this elution approach to probe ligand-target protein interactions through different assays (ligand characterization by direct assays, ligand ranking, displacement) (Temporini et al, 2013; de Moraes et al, 2014a; Ciesla et al, 2016; Chen X. et al, 2017; Yang Y.-X. et al, 2017), while other studies have used this elution mode to characterize the physicochemical properties of the target protein-containing solid support associated with the zonal elution mode for affinity and displacement studies (Habicht et al, 2015; Ma et al, 2015; Guo et al, 2017; Liu G. et al, 2017; Tao et al, 2018a). The next section discusses the potential of the state-of-the-art zonal elution to probe ligand-target protein interactions.…”

Section: On-line Approachesmentioning

confidence: 99%

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

2019

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Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational changes. Therefore, bioanalytical tools to screen new ligands have focused mainly on probing ligand-target interactions. These interactions have been evaluated by using solid-supported proteins, which provide advantages like increased protein stability and easier protein extraction from the reaction medium, which enables protein reuse. In some specific approaches, precisely in the ligand fishing assay, the bioanalytical method allows the ligands to be directly isolated from complex mixtures, including combinatorial libraries and natural products extracts without prior purification or fractionation steps. Most of these screening assays are based on liquid chromatography separation, and the binding events can be monitored through on-line or off-line methods. In the on-line approaches, solid supports containing the immobilized biological target are used as chromatographic columns most of the time. Several terms have been used to refer to such approaches, such as weak affinity chromatography, high-performance affinity chromatography, on-flow activity assays, and high-performance liquid affinity chromatography. On the other hand, in the off-line approaches, the binding event occurs outside the liquid chromatography system and may encompass affinity and activity-based assays in which the biological target is immobilized on magnetic particles or monolithic silica, among others. After the incubation step, the supernatant or the eluate from the binding assay is analyzed by liquid chromatography coupled to various detectors. Regardless of the selected bioanalytical approach, the use of solid supported proteins has significantly contributed to the development of automated and reliable screening methods that enable ligands to be isolated and characterized in complex matrixes without purification, thereby reducing costs and avoiding time-laborious steps. This review provides a critical overview of recently developed assays.

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Section: On-line Approachesmentioning

confidence: 99%

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

2019

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“…These results suggested the presence of specific sites on the surface of silica particles for GSF. These selective binding sites enhanced the adsorption of analyte from complex samples and decreased the binding of interfering substances [38].…”

Section: Selectivity Characteristics Of Smipsmentioning

confidence: 99%

Development of Surface Molecularly Imprinted Polymers as Dispersive Solid Phase Extraction Coupled with HPLC Method for the Removal and Detection of Griseofulvin in Surface Water

Bashir

Luo

Chen

et al. 2019

IJERPH

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Griseofulvin (GSF) is clinically employed to treat fungal infections in humans and animals. GSF was detected in surface waters as a pharmaceutical pollutant. GSF detection as an anthropogenic pollutant is considered as a possible source of drug resistance and risk factor in ecosystem. To address this concern, a new extraction and enrichment method was developed. GSF-surface molecularly imprinted polymers (GSF-SMIPs) were prepared and applied as solid phase extraction (SPE) sorbent. A dispersive solid phase extraction (DSPE) method was designed and combined with HPLC for the analysis of GSF in surface water samples. The performance of GSF-SMIPs was assessed for its potential to remove GSF from water samples. The factors affecting the removal efficiency such as sample pH and ionic strength were investigated and optimized. The DSPE conditions such as the amount of GSF-SMIPs, the extraction time, the type and volume of desorption solvents were also optimized. The established method is linear over the range of 0.1–100 µg/mL. The limits of detection and quantification were 0.01 and 0.03 µg/mL respectively. Good recoveries (91.6–98.8%) were achieved after DSPE. The intra-day and inter-day relative standard deviations were 0.8 and 4.3% respectively. The SMIPs demonstrated good removal efficiency (91.6%) as compared to powder activated carbon (67.7%). Moreover, the SMIPs can be reused 10 times for water samples. This is an additional advantage over single-use activated carbon and other commercial sorbents. This study provides a specific and sensitive method for the selective extraction and detection of GSF in surface water samples.

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“…Clenbuterol, a β 2 -adrenoceptor agonist, has raised worldwide concern due to its widespread applications as remedial drugs for the treatment of bronchial diseases (e.g., asthma, pulmonary emphysema, and chronic bronchitis) [1], and even more extensive attention is paid to its function as “lean meat powder”, which can stimulate the transformation of fat tissue to muscular tissue in livestock [2]. Since lean meat is the more favorable food relative to the fat, many illegal producers are motivated to misapply clenbuterol for the stimulation of lean meat growth.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular imprinting technologies are widely employed to render diverse materials selective to a target species [2,24]. Molecularly imprinted polymers (MIPs) are examples of considerable concern for various applications including clinical diagnostics [25], cell and tissue imaging [26], solid-phase extraction [27], catalysis [28], sensing [29], drug loading and delivery [30], etc.…”

Section: Introductionmentioning

confidence: 99%

Selective Enrichment of Clenbuterol onto Molecularly Imprinted Polymer Microspheres with Tailor-made Structure and Oxygen Functionalities

et al. 2019

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The noxious clenbuterol misapplied as the feed additive has posed an enormous threat to humans who actively rely on the food chains with high potential of contamination by clenbuterol, such as pork and beef. It is, therefore, highly desirable to develop novel materials and strategies for dealing with the clenbuterol. Herein, functional polymer microspheres prepared by Pickering emulsion polymerization were explored for the selective enrichment of the clenbuterol, and their structure and oxygen functionalities could be tailor-made by a molecular imprinting process. The clenbuterol imprinting was adequately demonstrated to not only increase the particle size (~52 nm vs. ~42 nm) and create cavities for the accommodation of the clenbuterol molecules, but also reduce the oxygen functionalities of the resulting molecularly imprinted polymer microspheres (MIPMs) by approximately 4 at.%, which is believed to correlate with the high specificity of the MIPMs. Various characterization methods were employed to evidence these findings, including scanning electron microscopy, BET measurements, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and elemental mapping examination. More importantly, the MIPMs showed a markedly superior enrichment capability towards clenbuterol to the counterpart, that is, non-molecularly imprinted polymer microspheres (NIPMs). Compared to the NIPMs without specificity for clenbuterol, the MIPMs exhibited an impressive selectivity to clenbuterol, with the relative selectivity coefficient (k′) values largely exceeding 1, thus corroborating that the useful molecular imprinting led to the generation of the binding sites complementary to the clenbuterol molecule in the size and functionalities. The MIPMs were also employed as the stationary phase to fabricate molecularly imprinting solid-phase extraction column, and the spike recovery was demonstrated to be not significantly decreased even after nine cycles. Furthermore, the reliability of the method was also evidenced through the comparison of the MIPMs prepared from different batches.

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Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples

Cited by 48 publications

References 27 publications

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

Development of Surface Molecularly Imprinted Polymers as Dispersive Solid Phase Extraction Coupled with HPLC Method for the Removal and Detection of Griseofulvin in Surface Water

Selective Enrichment of Clenbuterol onto Molecularly Imprinted Polymer Microspheres with Tailor-made Structure and Oxygen Functionalities

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