Development of ligustrazine hydrochloride carboxymethyl chitosan and collagen microspheres: Formulation optimization, characterization, and vitro release

Abstract

This study investigates the preparation of ligustrazine hydrochloride carboxymethyl chitosan and collagen microspheres. This experiment investigates effects of the ratio of carboxymethyl chitosan and collagen blend, water to oil ratio, stirring speed, and other factors on the microsphere properties. The experiment had the following conditions: a 1:2 proportion of carboxymethyl chitosan and collagen, a 1:2 proportion of drugs and materials, a 5:1 proportion of oil phase and water phase, 0.5% of span80, a 600r/min stirring speed, 3 ml of a cross-linking agent, 3 h of crosslinking curing, 1.25 § 0.05 mm diameter LTH microcapsules, a 54.08% envelop rate, and a 14.16% carrier rate. The microspheres release rate reached 66% within 1 h, then steadily released within 5 h in vitro. The experimental results showed that the ligustrazine hydrochloride microsphere production process was stable and exhibited a good release effect compared with other ligustrazine hydrochloride tablets and pills.

Application of Biomedical Microspheres in Wound Healing

“…When collagen microspheres are prepared by water-in-oil-in-water double emulsion solvent evaporation technology, the microspheres with different diameters, morphology and encapsulation rate can be prepared according to different collagen concentration and emulsification times. The higher the percentage of collagen, the longer the drug release curve [ 103 , 104 , 105 ]. Although there are many studies on collagen materials, there are not many clinical applications.…”

A Novel Method for Preparation of Carrageenan/Fish Scale Collagen/Allopurinol Biocomposite Film

“…As compared to the allopurinol released from CCA biocomposite films which were prepared by solution casting in our previous report [50], a similar tendency can be observed, meaning that the amount of allopurinol released from composite films in the pH 7.4 solution was higher than that in the pH 2.0 solution. This can be explained by the protonation of amine groups of collagen, which leads to the formation of a proton layer around the film, resulting in inhibiting the diffusion of a drug through the polymer films into the solution [23,31]. Moreover, the swelling of biocomposite films in the pH 7.4 solution is better than that in the pH 2.0 solution.…”

“…for loading a number of drugs such as antibacterial drugs, anti-inflammatory drugs, tobramycin or ciprofloxacin, doxorubicin, and gentamicin. [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. Carrageenan, a sulfated polysaccharide obtained from seaweed, has been also recognized as an effective matrix for drug delivery [6,7,[24][25][26][27][28][29][30][31][32][33].…”

Fabrication of Ligusticum chuanxiong polylactic acid microspheres: A promising way to enhance the hepatoprotective effect on bioactive ingredients

“…LCE-PLA in-vitro release behaviors were determined according to the method of Surwase, Munot, Idage and Idage (2017). Fifty mg of microspheres was suspended in different phosphate buffer solutions (pH 1.2, pH 6.8, pH 7.4) to make the microspheres solution, then put the solutions into 3 dialysis bags (MW, 14000 Da) (Lin, Huo, Qin, Zhao, & Tao, 2017). Afterwards, the dialysis bags were put into 28 mL phosphate buffer (pH 1.2, pH 6.8, pH 7.4) and maintained at 37±1 °C with 100 rpm agitating for 10 hours.…”