Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy

Ciani, Ilenia; Schulze, Holger; Corrigan, Damion K.; Henihan, Grace; Giraud, G.; Terry, Jonathan G.; Walton, A.J.; Pethig, Ronald; Ghazal, Peter; Crain, Jason; Campbell, Colin J.; Bachmann, Till T.; Mount, Andrew R.

doi:10.1016/j.bios.2011.11.004

Cited by 93 publications

(50 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the authors, the direct detection of these three biomarkers in synthetic wound fluid demonstrated the performance in a biologically relevant matrix under ambient conditions [55]. Another advantage of these biosensors was their viable multi-parameter sensing with a low LOD in the picomolar range.…”

Section: Antibodiesmentioning

confidence: 98%

“…Immunosensors were essentially developed for clinical diagnosis, as shown in Table 2 {e.g., detection of human serum albumin [54], cancer biomarkers [56,57,59], infection biomarkers [55], HIV p24 [47], and C-reactive protein [9,10]}.…”

Section: Antibodiesmentioning

confidence: 99%

“…Immunosensors for the direct detection of three wound-infection biomarkers based on screen-printed electrodes ( Table 2) were fabricated [55] as cheap, commercially available, and compatible with disposable sensing. According to the authors, the direct detection of these three biomarkers in synthetic wound fluid demonstrated the performance in a biologically relevant matrix under ambient conditions [55].…”

Section: Antibodiesmentioning

confidence: 99%

See 2 more Smart Citations

Recent developments in recognition elements for chemical sensors and biosensors

Justino

Freitas

Pereira

et al. 2015

TrAC Trends in Analytical Chemistry

269

118

View full text Add to dashboard Cite

Section: Antibodiesmentioning

confidence: 98%

Section: Antibodiesmentioning

confidence: 99%

Section: Antibodiesmentioning

confidence: 99%

See 1 more Smart Citation

Recent developments in recognition elements for chemical sensors and biosensors

Justino

Freitas

Pereira

et al. 2015

TrAC Trends in Analytical Chemistry

269

118

View full text Add to dashboard Cite

“…Electrochemical impedimetric spectroscopy (EIS) is a very sensitive and nondestructive electrochemical technique by which we can measure the interaction between antigen and its specific antibody without using labeled enzyme, as needed for ELISA [18,21e23] Compared with other sensors, impedimetric immunosensor is characterized by label-free, simple, time saving, requiring no special reagents, and can be used at lower potentials that avoid side reactions [18,24,25]. In impedance spectroscopy we apply a small sinusoidal alternating voltage (with any dc bias voltage if needed) and the resulting alternating current response is used to calculate the complex impedance (similar to resistance) at each of the applied frequencies.…”

Section: Introductionmentioning

confidence: 99%

Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Gupta

Mitra

2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

“…The charge transport capacity of the electrodes of these sensors can be measured by cyclic voltammetry (CV) or as a change in the electrical impedance by electrochemical impedance spectroscopy (EIS). These sensors have been recently used in a variety of point-of-care detection devices, and have shown to be promising for liver function, 33 wound infection biomarkers 34 and cancer biomarkers 35,36 detection. This review attempts to describe the migration of electrochemical techniques such as CV and EIS from characterization to point-of-care detection techniques.…”

mentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

View full text Add to dashboard Cite

Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

show abstract

Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy

Cited by 93 publications

References 37 publications

Recent developments in recognition elements for chemical sensors and biosensors

Recent developments in recognition elements for chemical sensors and biosensors

Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Electro-Immuno Sensors: Current Developments and Future Trends

Contact Info

Product

Resources

About