Development of immunoassay based on rational hapten design for sensitive detection of pendimethalin in environment

Huang, Lianrun; Chen, He; Cui, Pengcheng; Yuan, Ding; Wang, Minghua; Hua, Xiude

doi:10.1016/j.scitotenv.2022.154690

Cited by 22 publications

(7 citation statements)

References 25 publications

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“…The specificity and affinities of the resulting antibodies are well known to be influenced by the hapten structure . Therefore, the hapten design should closely resemble the target molecule with regard to size, steric conformation, electrical configuration, and hydrophobic characteristics.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The specificity and affinities of the resulting antibodies are well known to be influenced by the hapten structure. 29 Therefore, the hapten design should closely resemble the target molecule with regard to size, steric conformation, electrical configuration, and hydrophobic characteristics. For small molecules such as 6-BA (MW225.1), a reasonable spacer arm should be added to back up a specific epitope far from the carrier protein and enhance exposure to the animal immune system.…”

Section: Resultsmentioning

confidence: 99%

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Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Quan

Liu

et al. 2023

J. Agric. Food Chem.

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6-Benzylaminopurine (6-BA), a plant growth regulator with cytokinin-like properties, was recently reported to be illegally used in bean sprouts to increase their commercial appearance. It is still nevertheless challenging to quickly detect this adulteration. In this work, four novel haptens (haptens 1−4) of 6-BA were rationally designed with computer-assisted modeling analysis and then synthesized for use as immunizing haptens to produce antibodies. One of two obtained antibodies showed high sensitivity and specificity toward 6-BA. Based on the most sensitive anti-6-BA antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was performed, which demonstrated a 50% inhibition concentration (IC 50 ) of 1.18 μg/L and a limit of detection of 0.075 μg/L. The average recoveries of this icELISA for 6-BA of spiked samples ranged from 87.2 to 95.0% with a coefficient of variation of less than 8.7%. Furthermore, the blind samples were detected simultaneously by the method and HPLC-MS/MS, and the results showed good agreement with each other. Therefore, the proposed icELISA can facilitate the rapid surveillance screening of adulterated 6-BA in sprout vegetables.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Quan

Liu

et al. 2023

J. Agric. Food Chem.

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“…The antigens were conjugated using diazotization and activated ester methods according to previous publications [ 16 , 22 ]. Because it can expose the unique portions of CYP and prevent haptens from folding onto the carrier protein, a short space arm of immunogen hapten proved to be helpful for mAb development [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…The alternative to instrument methods is immunoassays, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFIAs) [ 13 , 14 , 15 ]. Based on antibody–antigen interactions, ELISA methods have been used for the detection of pyrethroid residue with the advantages of high sensitivity, rapidity, low cost, high-throughput and suitability for on-site analysis [ 16 ]. Xu et al established indirect competitive ELISA (ic-ELISA) based on a monoclonal antibody for CYP determination with a half inhibition concentration (IC 50 ) of 1.7 ng/mL [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples

Huang

Zhang

et al. 2022

Biosensors

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Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.

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“…In addition, this dinitroaniline herbicide is also less photodegradable than other dinitroanilines [5], which can cause its accumulation in agricultural soils [6,7] and aquatic ecosystems [8], affecting other nontarget organisms [9,10], including humans. In fact, pendimethalin has been classified as a possible human carcinogen [11,12] with negative effects on vasculature formation [13].…”

Section: Introductionmentioning

confidence: 99%

Reduction in the Use of Some Herbicides Favors Nitrogen Fixation Efficiency in Phaseolus vulgaris and Medicago sativa

Paniagua-López

Jiménez-Pelayo

Gómez-Fernández

et al. 2023

Plants

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In recent decades, the quality of agricultural soils has been seriously affected by the excessive application of pesticides, with herbicides being one of the most abundant. Continuous use of herbicides alters the soil microbial community and beneficial interactions between plants and bacteria such as legume-rhizobia spp. symbiosis, causing a decrease in the biological nitrogen fixation, which is essential for soil fertility. Therefore, the aim of this work was to study the effect of two commonly used herbicides (pendimethalin and clethodim) on the legume-rhizobia spp. symbiosis to improve the effectiveness of this process. Phaseolus vulgaris plants grown in pots with a mixture of soil:perlite (3:1 v/v), showed a 44% inhibition of nitrogen fixation rate with pendimethalin. However, clethodim, specifically used against monocots, did not induce significant differences. Additionally, we analyzed the effect of herbicides on root exudate composition, detecting alterations that might be interfering with the symbiosis establishment. In order to assess the effect of the herbicides at the early nodulation steps, nodulation kinetics in Medicago sativa plants inoculated with Sinorhizobium meliloti were performed. Clethodim caused a 30% reduction in nodulation while pendimethalin totally inhibited nodulation, producing a reduction in bacterial growth and motility as well. In conclusion, pendimethalin and clethodim application reduced the capacity of Phaseolus vulgaris and Medicago sativa to fix nitrogen by inhibiting root growth and modifying root exudate composition as well as bacterial fitness. Thus, a reduction in the use of these herbicides in these crops should be addressed to favor a state of natural fertilization of the soil through greater efficiency of leguminous crops.

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Development of immunoassay based on rational hapten design for sensitive detection of pendimethalin in environment

Cited by 22 publications

References 25 publications

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Authenticating Emergent Adulterant 6-Benzylaminopurine in Bean Sprouts: Virtual Hapten Similarity Enhanced Immunoassay

Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples

Reduction in the Use of Some Herbicides Favors Nitrogen Fixation Efficiency in Phaseolus vulgaris and Medicago sativa

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