Screening
for ″zero tolerance″ β-agonists requires
broad-specificity and sensitivity methods. Herein, R-(−)-salbutamol (SAL) is chirally separated and designed as
a hapten, and a monoclonal antibody (mAb) was first prepared with
an IC50 of 0.27 ng/mL, which can recognize 38 β-agonists
simultaneously. The broad-specificity of chiral mAb was explored by
molecular simulation technology. Magnetic nanoparticles (MNPs) were
then synthesized and applied as a signal tracer to develop a lateral
flow immunoassay (LFIA). The limits of detection of MNPs-LFIA for
SAL in swine urine and pork were 0.05 and 0.09 μg/kg, which
was (2–125)-fold lower than that of the reported LFIAs. The
recoveries were between 95.8 and 116.7%, with the coefficient of variation
from 2.7 to 15.4%. Parallel analysis of 44 samples by commercial ELISA
kits confirmed the reliability. Therefore, our work not only provides
a broad-specificity and ultrasensitive method for β-agonists
but also suggests that chirality is the new general theory that guided
the rational hapten design.
6-Benzylaminopurine (6-BA), a plant growth regulator with cytokinin-like properties, was recently reported to be illegally used in bean sprouts to increase their commercial appearance. It is still nevertheless challenging to quickly detect this adulteration. In this work, four novel haptens (haptens 1−4) of 6-BA were rationally designed with computer-assisted modeling analysis and then synthesized for use as immunizing haptens to produce antibodies. One of two obtained antibodies showed high sensitivity and specificity toward 6-BA. Based on the most sensitive anti-6-BA antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was performed, which demonstrated a 50% inhibition concentration (IC 50 ) of 1.18 μg/L and a limit of detection of 0.075 μg/L. The average recoveries of this icELISA for 6-BA of spiked samples ranged from 87.2 to 95.0% with a coefficient of variation of less than 8.7%. Furthermore, the blind samples were detected simultaneously by the method and HPLC-MS/MS, and the results showed good agreement with each other. Therefore, the proposed icELISA can facilitate the rapid surveillance screening of adulterated 6-BA in sprout vegetables.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.