Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea

Fang, Yalin; Wang, Zian; Quan, Qiqi; Li, Zhaodong; Pan, Kangliang; Lei, Yi; Yao, Xiaojun; Li, Xiangmei; Shen, Xing; Koidis, Anastasios; Lei, Hongtao

doi:10.1016/j.foodchem.2022.135065

Cited by 9 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although specific chemical interactions make the binding sites of antibodies available, the process is more complex than that involved in the physical interaction through electrostatic adsorption. AuNPs are the most common labels in ICAs, 20 and direct conjugation through electrostatic adsorption of antibodies and AuNPs is used in ICA 21–24 . Consequently, the performance of ICA for small‐molecule targets such as prohibited substances, contaminated antibiotics, and pesticides with similar analogues also needs to be investigated for possible cross‐reactivity alteration.…”

Section: Introductionmentioning

confidence: 99%

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Chuphol

Nokkaew

Makkliang

et al. 2023

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction: Miroestrol (Mi) and deoxymiroestrol (Dmi) are trace, yet potent, phytooestrogens found in white Kwao Krua [Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham, PM]. However, the analysis of these substances is difficult because of complex matrix effects and their various analogues. In addition, alteration in the cross-reactivity of a gold nanoparticle (AuNP)-based immunochromatographic assay (ICA) resulting from the electrostatic adsorption between antibodies and AuNPs has not yet been evaluated.Objectives: This study aims to develop, characterise, and validate ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi (MD-mAb). Materials and Methods:The ICA performance was validated for cross-reactivity and performance in comparison with those of indirect competitive enzyme-linked immunosorbent assays (icELISAs) with MD-mAb and mAb exhibiting specificity against Mi (Mi-mAb).Results: The ICA showed a limit of detection (LOD) at 1 and 16 μg/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower (6.25%) than that observed with the icELISA (120%). Cross-reactivity of ICA against other compounds of the PM was also correlated with those of icELISA; no false-positive/ negative results were observed. The repeatability and reproducibility of the ICA were confirmed. The results obtained using ICA in samples of PM are correlated with the concentrations determined through icELISAs. Conclusion:An ICA with MD-mAb was constructed and validated. However, direct conjugation via the electrostatic adsorption of mAb-AuNPs was expected to alter the cross-reactivity of ICA, especially that of the analyte analogue Dmi.

show abstract

Section: Introductionmentioning

confidence: 99%

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Chuphol

Nokkaew

Makkliang

et al. 2023

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…In addition, although AA can completely align with H1 and AA-BA (Figure S2), there were changes in the 14N charge caused by the absence of 16C, resulting in low values of CR for mAb produced by H1 and AA-GA. This hapten design strategy is similar to that of Fang et al reported . They prepared an antibody against aminopyrine with IC 50 of 3.00 ng/mL by a novel hapten (2-(4-(((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1 H -pyrazol-4-yl)(methyl)amino)methyl)phenyl)acetic acid) (Table ).…”

Section: Resultsmentioning

confidence: 98%

Development of a Lateral Flow Immunoassay Based on a Highly Specific Monoclonal Antibody To Detect 4-Methylaminoantipyrine

Wang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural analogues, especially metabolites of target compounds, the preparation of highly specific antibodies is crucial. Preserving the characteristic structure of a target compound when designing a hapten is important when preparing highly specific antibodies. Here, we designed a novel hapten, 4-(((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4yl)amino)methyl)benzoic acid, named AA-BA, to improve the specificity of antibodies for detection of 4-methylaminoantipyrine (MAA), a residual marker of dipyrone, an important antipyretic-analgesic and anti-inflammatory drug. The structural features of the hapten remained almost the same as those of MAA. After experimental validation, monoclonal antibody 6A4 (mAb 6A4) was prepared with the half maximal inhibitory concentration (IC 50 ) value of 4.03 ng/mL and negligible cross-reactivity with dipyrone metabolites and other antibiotics. In addition, a specific lateral flow immunoassay (LFA) strip based on colloidal gold was developed for screening MAA with a cutoff value of 25 ng/mL in milk. The developed LFA is a useful tool for rapid and accurate detection of MAA.

show abstract

“…2) and specific polyclonal antibodies against AP were derived from previous work in our laboratory. 20 The immunogen (Hapten A-LF) was obtained by hapten A coupled with lactoferrin (LF), and a polyclonal antibody was obtained by immunizing New Zealand white rabbits. The concentration of antibody was 62.5 ng mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Development of a highly sensitive immunoassay for detecting aminopyrine abuse in herbal tea

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea

Cited by 9 publications

References 23 publications

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Development of a Lateral Flow Immunoassay Based on a Highly Specific Monoclonal Antibody To Detect 4-Methylaminoantipyrine

Development of a highly sensitive immunoassay for detecting aminopyrine abuse in herbal tea

Contact Info

Product

Resources

About