Development of Hydrogel Lenses with Surface‐immobilized <scp>PEG</scp> Layers to Reduce Protein Adsorption

Jee, Jun‐Pil; Kim, Ho‐Joong

doi:10.1002/bkcs.10545

Cited by 16 publications

(11 citation statements)

References 40 publications

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“…The ability of the prepared hydrogels to suppress protein adsorption was assessed according to the literature . Herein, the prepared hydrogels were initially placed in a vial containing 10 mL of phosphate‐buffered saline (PBS, pH 7.4) and incubated in a shaking incubator at room temperature and 150 rpm for 24 h. Subsequently, two artificial tear solutions containing 3.88 g/L of bovine serum albumin (BSA) and 1.20 g/L of chicken egg‐white lysozyme in PBS were separately prepared.…”

Section: Methodsmentioning

confidence: 88%

“…These biological reactions sharply reduce the properties of the biomaterials. For instance, protein deposition onto contact lenses reduces their clarity, shortens the life‐span, and causes discomfort . Hence, there is considerable interest for the development of novel biomaterials with improved resistance to foulants such as proteins and bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Protein Adsorption and Bacterial Adhesion Resistance of Cross‐linked Copolymer Hydrogels Based on Poly(2‐methacryloyloxyethyl phosphorylcholine) and Poly(2‐hydroxyethyl methacrylate)

Vales

Jee

Lee

et al. 2020

Bulletin Korean Chem Soc

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Herein, the preparation of the cross-linked copolymer hydrogels composed of various weight ratios of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 2-hydroxyethyl methacrylate (HEMA) monomers employing free-radical polymerization was reported. The integration of the MPC monomer into HEMAbased hydrogels showed good transmittance (>90%) and enhanced the water content retention (79-202%) relative to those of pure HEMA-based hydrogel. Notably, the MPC-containing hydrogels (MPC-H) exhibited the improved anti-biofouling properties due to the formation of a compact hydration layer produced by the biomimetic MPC units at the hydrogel surface. MPC-H exhibited a decrease in the bovine serum albumin (BSA) and lysozyme adsorption of approximately 40-70% and 44-65%, respectively, relative to those of control hydrogel without MPC monomer. The results presented herein demonstrate the potential of expending biocompatible monomers such as HEMA and MPC to efficiently generate a biomaterial that possesses both bio-membrane mimicking character and protein and bacterial resistant properties for various industrial and biomedical applications.

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Section: Methodsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Protein Adsorption and Bacterial Adhesion Resistance of Cross‐linked Copolymer Hydrogels Based on Poly(2‐methacryloyloxyethyl phosphorylcholine) and Poly(2‐hydroxyethyl methacrylate)

Vales

Jee

Lee

et al. 2020

Bulletin Korean Chem Soc

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“…The anti-fouling properties of poly(ethylene glycol) are mainly due to their high chains mobility, high free volume and steric hindrance. In particular, PEG chains have hydrophilic repeating units, flexible conformation, and even some branched structure derivatives associated with steric hindrance (Jee and Kim 2015). Using poly(ethylene glycol) in formulation provides lenses with higher hydophilicity and anti-fouling properties.…”

Section: Resultsmentioning

confidence: 99%

Reduction in protein absorption on ophthalmic lenses by PEGDA bulk modification of silicone acrylate-based formulation

et al. 2019

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The absorption of protein and formation of biofilms on the surface of ophthalmic lenses is one of the factors that destroy their useful performance by causing severe visual impairment, inflammation, dryness and ultimate eye discomfort. Therefore, eye lenses need to be resilient to protein absorption, which is one of the opacity factors in minimizing protein absorption on the lenses. The purpose of this study was to investigate and reduce sediment biotransformation on the surface of the semi-hardened lens based on acrylate by bulk-free radical polymerization method. In this respect, the effect of poly(ethylene glycol) diacrylate (PEGDA) with two different molecular weights of 200 and 600 g/mol on the surface roughness, protein absorption, and hydrophilicity of the lenses were studied. The surface hardness of the lenses, on shore D scale, was measured using a durometer hardness test. The presence of higher molecular weight of PEGDA hydrophilic polymeric monomers reduced the hardness of the lenses. The effect of introducing PEGDA, with two molecular weights, into lens fabrication formulations was studied with respect to their water content parameters and hydrophilicity. The presence of a crosslinker such as poly(ethylene glycol) diacrylates, at two different molecular weights, increased the water content and hydrophilicity of the produced lenses. Surface roughness is associated with the formation of bio-film and accumulation of microorganisms on the surface. Due to the roughness of the lens surface developed in this research, the lenses containing PEGDA 600 exhibited less roughness compared to that of PEGDA 200, which could also affect the absorption of protein. Therefore, according to the results of protein absorption test, the PEGDA 600 lenses showed lower protein absorption, which could be due to their high degree of water absorption and hydrophilicity.

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“…Protein desorption was measured previously described [51]. The prepared hydrogels were initially immersed in 10 mL PBS (pH 7.4) and incubated in an incubator shaker at room temperature and 150 rpm for 24 h. Two artificial tear solutions containing 3.88 g/L of bovine serum albumin (BSA) and 1.20 g/L of chicken egg-white lysozyme in PBS were prepared.…”

Section: Protein Desorption From the P(hema)/p(mpc) Hydrogelsmentioning

confidence: 99%

Development of Poly(2-Methacryloyloxyethyl Phosphorylcholine)-Functionalized Hydrogels for Reducing Protein and Bacterial Adsorption

et al. 2020

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A series of hydrogels with intrinsic antifouling properties was prepared via surface-functionalization of poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based hydrogels with the biomembrane-mimicking zwitterionic polymer, poly(2-methacryloyloxyethyl phosphorylcholine) [p(MPC)]. The p(MPC)-modified hydrogels have enhanced surface wettability, high water content retention (61.0%–68.3%), and good transmittance (>90%). Notably, the presence of zwitterionic MPC moieties at the hydrogel surfaces lowered the adsorption of proteins such as lysozyme and bovine serum albumin (BSA) by 73%–74% and 59%–66%, respectively, and reduced bacterial adsorption by approximately 10%–73% relative to the unmodified control. The anti-biofouling properties of the p(MPC)-functionalized hydrogels are largely attributed to the dense hydration layer formed at the hydrogel surfaces by the zwitterionic moieties. Overall, the results demonstrate that biocompatible and antifouling hydrogels based on p(HEMA)-p(MPC) structures have promising potential for application in biomedical materials.

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Development of Hydrogel Lenses with Surface‐immobilized PEG Layers to Reduce Protein Adsorption

Cited by 16 publications

References 40 publications

Protein Adsorption and Bacterial Adhesion Resistance of Cross‐linked Copolymer Hydrogels Based on Poly(2‐methacryloyloxyethyl phosphorylcholine) and Poly(2‐hydroxyethyl methacrylate)

Protein Adsorption and Bacterial Adhesion Resistance of Cross‐linked Copolymer Hydrogels Based on Poly(2‐methacryloyloxyethyl phosphorylcholine) and Poly(2‐hydroxyethyl methacrylate)

Reduction in protein absorption on ophthalmic lenses by PEGDA bulk modification of silicone acrylate-based formulation

Development of Poly(2-Methacryloyloxyethyl Phosphorylcholine)-Functionalized Hydrogels for Reducing Protein and Bacterial Adsorption

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