Protein Adsorption and Bacterial Adhesion Resistance of Cross‐linked Copolymer Hydrogels Based on Poly(2‐methacryloyloxyethyl phosphorylcholine) and Poly(2‐hydroxyethyl methacrylate)

Abstract

Herein, the preparation of the cross-linked copolymer hydrogels composed of various weight ratios of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 2-hydroxyethyl methacrylate (HEMA) monomers employing free-radical polymerization was reported. The integration of the MPC monomer into HEMAbased hydrogels showed good transmittance (>90%) and enhanced the water content retention (79-202%) relative to those of pure HEMA-based hydrogel. Notably, the MPC-containing hydrogels (MPC-H) exhibited the improved anti-biofouling properties due to the formation of a compact hydration layer produced by the biomimetic MPC units at the hydrogel surface. MPC-H exhibited a decrease in the bovine serum albumin (BSA) and lysozyme adsorption of approximately 40-70% and 44-65%, respectively, relative to those of control hydrogel without MPC monomer. The results presented herein demonstrate the potential of expending biocompatible monomers such as HEMA and MPC to efficiently generate a biomaterial that possesses both bio-membrane mimicking character and protein and bacterial resistant properties for various industrial and biomedical applications.

Impact of polymeric films and hydrogels: Physical characteristics on bacterial growth

“…It was because of the high hydrophilic properties from the enriched hydrophilic shell of MPC, which had been caused by zwitterionic, representing bacteria's unfavorable binding on the hydrophilic surface. This study also confirmed the unfavorable adhesion of bacteria on the hydrophilic surface 97 . However, some hydrophobic polymers can cause membrane disruption and bacterial destruction.…”

“…This study also confirmed the unfavorable adhesion of bacteria on the hydrophilic surface. 97 However, some hydrophobic polymers can cause membrane disruption and bacterial destruction. The interaction of hydrophobic hydrogel and bacteria has been described by Irwansyah I.…”

Novel Trends in Hydrogel Development for Biomedical Applications: A Review

“…However, single pHEMA-based hydrogels have little commercial application; thus, numerous studies have been conducted to modify pHEMA structure with the aim of improving its properties, for example, using cross-linking agents such as ethylene glycol dimethacrylate (EGDMA) [ 41 ] and tetra(ethylene glycol) diacrylate (TEGDA) [ 42 , 43 ] to enhance its mechanical properties, or β-cyclodextrin-hyaluronan (β-CDcrHA) to reduce tear protein absorption in a contact lens [ 129 ]. Another approach is to copolymerize pHEMA with other polymers, commonly with polyacrylamide (PAA) [ 130 ] or ethylene glycol dimethacrylate (EGDMA) [ 40 ] to improve its mechanical properties, poly (ethylene glycole) diacrylate (PEGDA) to improve host biosensors or enhance its water absorption capacity [ 131 ], glycidyl methacrylate (GMA) [ 132 ] to facilitate cell attachment and proliferation, or 2-methacryloyloxyethyl phosphorylcholine (MPC) [ 133 ] to improve the water content retention and anti-biofouling properties. Moreover, other possibilities to improve pHEMA-based hydrogels’ performance for biomedical applications include the formation of composites, e.g., pHEMA with boric acid (BA), with interesting applications as soft contact lens material, as reported by Ulu et al (2018) [ 134 ], or the formation of IPNs with gelatin to enhance biological properties for in vitro and in vivo performance [ 135 ].…”

Synthesis and Properties of Hydrogels on Medical Titanium Alloy Surface by Modified Dopamine Adhesion

“…2-methacryloyloxyethyl phosphorylcholine (MPC) [ 31 , 32 , 33 , 34 ] is a zwitterion that is a good biocompatible substance. It has received widespread attention because of its hydrophilicity and ability to avoid protein adsorption and cell adhesion.…”