Development of High Purity Purification Method of Chondroitin Sulfate Extracted from Skate Cartilage

Jeong, Kap-Seop

doi:10.5762/kais.2016.17.6.9

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…Among the chromatographic methods, ion-exchange chromatography (IEC), fast protein liquid chromatography (FPLC) [ 59 ], low-pressure liquid chromatography (LPLC) [ 22 ], and high-performance gel permeation chromatography (HPGPC) [ 60 ] are commonly used. For the purification of CS, ion exchange resins, such as silica gel, were also used with traditional methods such as filtration [ 61 ]. The use of silica gel was found to improve the purity of the CS extraction process [ 62 ].…”

Section: Chondroitin Sulphatementioning

confidence: 99%

A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

Urbi

Azmi

Ming

et al. 2022

CIMB

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Chondroitin sulphate (CS) is one of the most predominant glycosaminoglycans (GAGs) available in the extracellular matrix of tissues. It has many health benefits, including relief from osteoarthritis, antiviral properties, tissue engineering applications, and use in skin care, which have increased its commercial demand in recent years. The quest for CS sources exponentially increased due to several shortcomings of porcine, bovine, and other animal sources. Fish and fish wastes (i.e., fins, scales, skeleton, bone, and cartilage) are suitable sources of CS as they are low cost, easy to handle, and readily available. However, the lack of a standard isolation and characterization technique makes CS production challenging, particularly concerning the yield of pure GAGs. Many studies imply that enzyme-based extraction is more effective than chemical extraction. Critical evaluation of the existing extraction, isolation, and characterization techniques is crucial for establishing an optimized protocol of CS production from fish sources. The current techniques depend on tissue hydrolysis, protein removal, and purification. Therefore, this study critically evaluated and discussed the extraction, isolation, and characterization methods of CS from fish or fish wastes. Biosynthesis and pharmacological applications of CS were also critically reviewed and discussed. Our assessment suggests that CS could be a potential drug candidate; however, clinical studies should be conducted to warrant its effectiveness.

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Section: Chondroitin Sulphatementioning

confidence: 99%

A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

Urbi

Azmi

Ming

et al. 2022

CIMB

View full text Add to dashboard Cite

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Hyaluronic acid and Chondroitin sulfate from marine and terrestrial sources: Extraction and purification methods

Abdallah

Fernández²,

Matias³

et al. 2020

Carbohydrate Polymers

111

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Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

Kim¹,

Lee²,

Shin³

et al. 2022

Korean J. Food Preserv.

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In this study, we developed and validated a high-performance liquid chromatography/photodiode array method for the quantification of glycine-proline-hydroxyproline (GPH) as a marker compound in skate skin collagen peptides (SCPs). The accurate molecular mass and amino acid sequence of this marker were determined using a QTOF mass spectrometer. Chromatographic separations were conducted using a 95:5 isocratic mobile phase of 0.1% trifluoroacetic acid (A) and acetonitrile/methanol (1:4 v/v, B) at a flow rate of 0.3 mL/min; the separated compounds were detected at 214 nm using a Jupiter® 4 μm Proteo 90Å column. The method was validated for specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. It was found to be linear in the range of 1.0-50.0 μg/mL, with a good correlation coefficient (R2=0.9999) and excellent specificity. The LOD and LOQ were 0.07 and 0.22 μg/mL, respectively. A recovery study determined the accuracy of the method, with an average recovery of 103.76%, 100.35% and 103.97% of the marker at 10, 15 and 25 μg/mL, respectively, and a precision study showed a relative standard deviation of less than 2%. Additionally, the DPPH and ABTS radical scavenging activities of the SCPs increased in a concentration-dependent manner, and UV-protective effect was confirmed by human keratinocyte (HaCaT cell) viability. Our study thus provides the foundation for developing functional foods or cosmetics using SCPs.

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Development of High Purity Purification Method of Chondroitin Sulfate Extracted from Skate Cartilage

Cited by 3 publications

References 5 publications

A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

Hyaluronic acid and Chondroitin sulfate from marine and terrestrial sources: Extraction and purification methods

Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

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