Development of functionalized superparamagnetic iron oxide nanoparticles for interaction with human cancer cells

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

“…13 Bare SPIO can subsequently be coated in monomers and polymers (Table 2) through non-specific adsorption following their purification to make them more suited for biomedical applications. 109 Alternatively, the monomers and polymers may be grafted to the nanoparticles during the precipitation step, although this will change the physical characteristics of the resulting nanoparticles. 13,32 One commonly used coating for bare SPIO is silica.…”

Biomedical nanoparticles modulate specific CD4+T cell stimulation by inhibition of antigen processing in dendritic cells

“…The zeta potential (surface charge) decreased from +16.7 mV to +6.7 mV, indicating significant coupling of both Oregon green and Alexa Fluor 680 fluorochromes to amine groups of the polymer coating. The zeta potential of derived particles remained slightly positive, which was proven to be very helpful to enable cellular internalization in previous studies (Petri-Fink et al 2005). Combination of a specifically designed polymer coating for SPIONs (e.g.…”

“…Alteration of immune responses by nanoparticles may represent a form of "nano-immuno-toxic" effect that may seriously undermine future efforts to develop novel therapeutic or diagnostic approaches. Utilizing prototypic fluorochrome-labelled model PVA-SPIONs previously evaluated in several preclinical studies, we herein provide data derived from in vitro studies of DCs treated with such nanoparticles (Petri-Fink et al 2005;Cengelli et al 2006;Hellstern et al 2006;Petri-Fink and Hofmann 2007;von Zur Muhlen et al 2007;Butoescu et al 2008;Petri-Fink et al 2008;Butoescu et al 2009; . Antigen-uptake, antigen-processing, and MHCII expression of MDDCs treated with PVA-SPIONs during 12 h. Endocytotic capacity (antigen uptake) and antigen processing were measured by incubation with a variety of fluorescent conjugates, as previously described (Wikstrom et al 2006;von Garnier et al 2007).…”

“…Polymer-coated SPIONs were produced as described elsewhere (Petri-Fink et al 2005). Magnetic nanoparticles were stabilized by a mixture of polyvinyl alcohol (Mowiol 3-85) and vinyl alcohol/vinyl amine copolymer (M12, vinyl alcohol/vinyl alcohol copolymer mass ratio).…”

Immobilization of biomolecules on the surface of inorganic nanoparticles for biomedical applications

“…The amount of CPT-USPIO taken up by human Me300 melanoma cells after 16 and 36 h as a function of the number of added nanoparticles was determined from the amount of cell-associated iron and cell-associated CPT fluorescence (figures 11(a) and (b)) as compared with that of the previously described aminoPVA-USPIOs [86]. After 16 h, the uptakes of aminoPVA-USPIOs and CPT-USPIO were comparable ( figure 11(a)).…”