Immobilization of biomolecules on the surface of inorganic nanoparticles for biomedical applications

Xing, Zhicai; Chang, Yongmin; Kang, Inn‐Kyu

doi:10.1088/1468-6996/11/1/014101

Cited by 48 publications

(33 citation statements)

References 92 publications

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“…Over the past decade, the interest for using carbon nanotubes (CNTs) functionalized with different types of biomolecules has increased [1][2][3]. The nanometer size and unique physical properties of CNTs make them attractive for anticancer drug delivery [4][5][6][7][8], molecular transport [9][10][11], and new therapeutic mechanisms [12,13].…”

Section: Introductionmentioning

confidence: 99%

Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered NMR spectroscopy

Castillo

Torres

Molina

et al. 2012

Carbon

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Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered nmr spectroscopy, Carbon (2012), doi: 10.1016/j.carbon. 2012.02.010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered nmr spectroscopy ABSTRACTA conjugate between single-walled carbon nanotubes, chitosan and folic acid has been prepared. It was characterized by diffusion ordered two-dimensional hydrogen-1 nuclear magnetic resonance and hydrogen-1 nuclear magnetic resonance spectroscopy which revealed the presence of a conjugate that was generated by the linkage between the carboxyl moiety of the folic acid and the amino group of the chitosan, which in turn was non-covalently bound to the single-walled carbon nanotubes. The obtained diffusion coefficient values demonstrated that free folic acid diffused more rapidly than the folic acid conjugated to single-walled carbon nanotubes-chitosan. The values of the proton signal of hydrogen-1 nuclear magnetic resonance spectroscopy and two-dimensional hydrogen-1 nuclear magnetic resonance spectroscopy further confirmed that the folic acid was conjugated to the chitosan, wrapping the single-walled carbon nanotubes.

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Section: Introductionmentioning

confidence: 99%

Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered NMR spectroscopy

Castillo

Torres

Molina

et al. 2012

Carbon

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“…Nanomaterials of carbon origin have been shown to have the sustained capacity due to the high immobilization capacity and strong binding and resistant to biochemical and microbiological conditions. Thus, the enzyme immobilized carbon-based nanomaterials are being used in therapeutic applications [18][19][20]. The mesoporous activated carbon (MAC) can also be regarded to possess the above characteristics of nano carbon matrix.…”

Section: Introductionmentioning

confidence: 99%

Production of lipase from Pseudomonas gessardii using blood tissue lipid and thereof for the hydrolysis of blood cholesterol and triglycerides and lysis of red blood cells

Ramani

Sekaran

2012

Bioprocess Biosyst Eng

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The study demonstrates the production of lipase (LIP) from Pseudomonas gessardii using blood tissue lipid as the substrate for the hydrolysis of blood cholesterol and triglycerides. The lipase was purified with the specific activity of 828 U/mg protein and the molecular weight of 56 kDa. The maximum lipase activity was observed at the pH 7.0 and the temperature 37 °C. The amino acid composition of purified lipase was determined by HPLC. The mesoporous activated carbon (MAC) was used for the immobilization of lipase for the repeated use of the enzyme catalyst. The K (m) value of immobilized lipase (MAC-LIP) and the free lipase (LIP) was 0.182 and 1.96 mM, respectively. The V (max) value of MAC-LIP and LIP was 1.33 and 1.26 mM/min, respectively. The MAC and MAC-LIP were characterized by scanning electron microscopy (SEM). The hydrolysis study showed 78 and 100% hydrolysis of triglycerides and cholesterol, respectively, for LIP and 84 and 100% hydrolysis of triglycerides and cholesterol, respectively, for MAC-LIP at the reaction time of 1 h. The effect of lipase on cell wall lysis was carried out on the RBCs of blood plasma. Interestingly, 99.9% lysis of RBCs was observed within 2 h. SEM images and phase contrast microscopy confirmed the lysis of RBCs. This work provides a potential biocatalyst for the hydrolysis of blood cholesterol and triglycerides.

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“…The thiol groups of the biomolecule react with the metal surface resulting in covalent bonds to the surface of the metal nanoparticle or metal film. Silica surfaces (such as silicon nanowires or silica nanospheres) contain silanol groups [94,95]. Here, heterobifunctional linkers are deployed, which contain silane groups on one end and a carboxyl or epoxide group on the other.…”

Section: Biofunctionalization Of Nanostructuresmentioning

confidence: 99%

Towards in vitro molecular diagnostics using nanostructures

Kurkina

Balasubramanian

2011

Cell. Mol. Life Sci.

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Nanostructures appear to be promising for a number of applications in molecular diagnostics, mainly due to the increased surface-to-volume ratio they can offer, the very low limit of detection achievable, and the possibility to fabricate point-of-care diagnostic devices. In this paper, we review examples of the use of nanostructures as diagnostic tools that bring in marked improvements over prevalent classical assays. The focus is laid on the various sensing paradigms that possess the potential or have demonstrated the capability to replace or augment current analytical strategies. We start with a brief introduction of the various types of nanostructures and their physical properties that determine the transduction principle. This is followed by a concise collection of various functionalization protocols used to immobilize biomolecules on the nanostructure surface. The sensing paradigms are discussed in two contexts: the nanostructure acting as a label for detection, or the nanostructure acting as a support upon which the molecular recognition events take place. In order to be successful in the field of molecular diagnostics, it is important that the nanoanalytical tools be evaluated in the appropriate biological environment. The final section of the review compiles such examples, where the nanostructure-based diagnostic tools have been tested on realistic samples such as serum, demonstrating their analytical power even in the presence of complex matrix effects. The ability of nanodiagnostic tools to detect ultralow concentrations of one or more analytes coupled with portability and the use of low sample volumes is expected to have a broad impact in the field of molecular diagnostics.

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Immobilization of biomolecules on the surface of inorganic nanoparticles for biomedical applications

Cited by 48 publications

References 92 publications

Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered NMR spectroscopy

Monitoring the functionalization of single-walled carbon nanotubes with chitosan and folic acid by two-dimensional diffusion-ordered NMR spectroscopy

Production of lipase from Pseudomonas gessardii using blood tissue lipid and thereof for the hydrolysis of blood cholesterol and triglycerides and lysis of red blood cells

Towards in vitro molecular diagnostics using nanostructures

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