Development of Fumonisin-Induced Hepatotoxicity and Pulmonary Edema in Orally Dosed Swine: Morphological and Biochemical Alterations

Gumprecht, Laura A.; Beasley, Val R.; Weigel, Ronald M.; Parker, Helen M.; Tumbleson, Mike E; Bacon, Charles W.; Meredith, Filmore I.; Haschek, Wanda M.

doi:10.1177/019262339802600610

Cited by 61 publications

(65 citation statements)

References 28 publications

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“…An increase in the concentration of Sa and the Sa/So ratio has also been observed in pigs fed 32 µmol of FB 1 /kg of diet (15). In the present study, concentrations of Sa as well as the Sa/So ratio showed a time-dependent elevation in the FB and FB-G groups, which agrees with studies by Smith et al (39) and Gumprecht et al (40). The levels of So were not different among the three groups throughout the majority of the present study (days 0, 5, and 15).…”

Section: Discussionsupporting

confidence: 93%

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Fernandez-Surumay

Osweiler²,

Yaeger³

et al. 2005

J. Agric. Food Chem.

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The effects of fumonisin B-glucose reaction products in swine diets was examined. Pigs were fed diets containing 528 µmol of total fumonisin B/kg (FB), 528 µmol of total FB-glucose adducts/kg (FB-G, 122 µmol of unreacted FB/kg), or 0 µmol of total FB/kg for 15 days to test the efficacy of the FB-G reaction products in detoxifying FB. Weight gain in FB pigs was lower than in FB-G or controls, which was correlated with feed intake reduction in FB pigs. Serum aspartate aminotransferase, γ-glutamyltransferase, and total bilirubin in FB pigs were higher than in FB-G or control pigs. Serum sphinganine/shingosine ratios in FB pigs were higher than in FB-G or control pigs. Microscopic examination of tissues from FB pigs showed generalized liver necrosis and apoptosis with marked cellular pleomorphism and disorganized hepatic cords. The liver and kidneys in the FB-G group appeared to be normal. Tissues of controls were free of lesions. Results suggest that dietary FB-G products are less toxic to swine and may provide an detoxification approach in instances of widespread FB grain contamination (p < 0.05). KeywordsInterdepartmental The effects of fumonisin B-glucose reaction products in swine diets was examined. Pigs were fed diets containing 528 µmol of total fumonisin B/kg (FB), 528 µmol of total FB-glucose adducts/kg (FB-G, 122 µmol of unreacted FB/kg), or 0 µmol of total FB/kg for 15 days to test the efficacy of the FB-G reaction products in detoxifying FB. Weight gain in FB pigs was lower than in FB-G or controls, which was correlated with feed intake reduction in FB pigs. Serum aspartate aminotransferase, γ-glutamyltransferase, and total bilirubin in FB pigs were higher than in FB-G or control pigs. Serum sphinganine/shingosine ratios in FB pigs were higher than in FB-G or control pigs. Microscopic examination of tissues from FB pigs showed generalized liver necrosis and apoptosis with marked cellular pleomorphism and disorganized hepatic cords. The liver and kidneys in the FB-G group appeared to be normal. Tissues of controls were free of lesions. Results suggest that dietary FB-G products are less toxic to swine and may provide an detoxification approach in instances of widespread FB grain contamination (p < 0.05).

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Section: Discussionsupporting

confidence: 93%

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Fernandez-Surumay

Osweiler²,

Yaeger³

et al. 2005

J. Agric. Food Chem.

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“…(46), liver damage occurs at 23 ppm (40), and pulmonary edema occurs at 175 ppm (40). Clinical chemistry profiles indicate that alkaline phosphatase is the most sensitive measure of fumonisin toxicity in pigs (20,40,46). The dose and the time exposure used in our study did not induce any change in serum biochemical parameters (data not shown) but did significantly increase bacterial colonization by pathogenic E. coli.…”

Section: Ingestion Of Fb 1 Increased Intestinal Colonization By E Comentioning

confidence: 67%

“…It induces leukoencephalomalacia in horses, pulmonary edema in pigs, and nephrotoxicity in rats, rabbits, lambs, and calves (3,14,21,22,32,54). In all species studied, both acute and chronic exposure to FB 1 are associated with alteration of sphingolipid metabolism and hepatotoxicity (9,20,21,26,44,46,48). FB 1 also has been implicated as a contributing factor in human esophageal cancers (45) and is a renal and hepatic carcinogen in male and female rats, respectively (22).…”

mentioning

confidence: 99%

Mycotoxin Fumonisin B ₁ Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs

Oswald¹,

Désautels

Laffitte³

et al. 2003

Appl Environ Microbiol

130

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Fumonisin B 1 (FB 1 ) is a mycotoxin that commonly occurs in maize. FB 1 causes a variety of toxic effects in different animal species and has been implicated as a contributing factor of esophageal cancers in humans. In the present study, we examined the effect of dietary exposure to FB 1 on intestinal colonization by pathogenic Escherichia coli associated with extraintestinal infection. Three-week-old weaned pigs were given FB 1 by gavage as a crude extract or as a purified toxin at a dose of 0.5 mg/kg of body weight daily for 6 days. On the last day of the toxin treatment, the pigs were orally inoculated with an extraintestinal pathogenic E. coli strain. All animals were euthanized 24 h later, necropsies were performed, and tissues were taken for bacterial counts and light microscopic examination. Ingestion of FB 1 had only a minimal effect on animal weight gain, did not cause any macroscopic or microscopic lesions, and did not change the plasma biochemical profile. However, colonization of the small and large intestines by an extraintestinal pathogenic E. coli strain was significantly increased. Our results show that FB 1 is a predisposing factor to infectious disease and that the pig can be used as a model for the study of the consequences of ingesting mycotoxin-contaminated food.

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“…The higher Sa levels in the FB 1 group were what we expected. Several studies had previously found a significant elevation of Sa after swine had been fed pure FB 1 at 0.002 µmol/g of diet for 5 days (40), FB 1 from culture material at 0.027 µmol/g for 1 day (41), or pure FB 1 at 0.013 µmol/g for 8 days (30). In contrast to Riley et al's findings (13), the serum Sa levels in the FB 1 -treated pigs of our study did not plateau before day 4, and the serum So levels did not continue to increase for the duration of the study.…”

Section: Discussionmentioning

confidence: 99%

Glucose Reaction with Fumonisin B₁Partially Reduces Its Toxicity in Swine

Fernandez-Surumay

Osweiler

Yaeger

et al. 2004

J. Agric. Food Chem.

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Acute and subacute intraperitoneal doses of fumonisin B1 (FB1) were administered to test the efficacy of the FB1-glucose reaction products in detoxifying FB1 in swine. In the acute study at 11 µmol of FB1/kg of body weight, five of six pigs administered FB1 and four of six pigs administered FB1-glucose died from acute pulmonary edema. Analysis of weight gain, serum aspartate aminotransferase and γ-glutamyltransferase, total cholesterol, and pathological evaluation did not provide evidence of protection against FB1 toxicity by the FB1-glucose reaction products. In the subacute study at 5.5 µmol of FB1/kg of body weight, one pig administered FB1 died from liver damage. Analysis of serum aspartate aminotransferase, γ-glutamyltransferase, and total bilirubin showed protection against FB1 toxicity by the FB1-glucose reaction products. The levels of sphinganine and sphinganine/sphingosine ratios in serum and liver as well as pathologic findings provided definitive evidence of protection against the FB1 toxic effects by this detoxification procedure (p < 0.05). Acute and subacute intraperitoneal doses of fumonisin B 1 (FB 1 ) were administered to test the efficacy of the FB 1 -glucose reaction products in detoxifying FB 1 in swine. In the acute study at 11 µmol of FB 1 /kg of body weight, five of six pigs administered FB 1 and four of six pigs administered FB 1 -glucose died from acute pulmonary edema. Analysis of weight gain, serum aspartate aminotransferase and γ-glutamyltransferase, total cholesterol, and pathological evaluation did not provide evidence of protection against FB 1 toxicity by the FB 1 -glucose reaction products. In the subacute study at 5.5 µmol of FB 1 /kg of body weight, one pig administered FB 1 died from liver damage. Analysis of serum aspartate aminotransferase, γ-glutamyltransferase, and total bilirubin showed protection against FB 1 toxicity by the FB 1 -glucose reaction products. The levels of sphinganine and sphinganine/sphingosine ratios in serum and liver as well as pathologic findings provided definitive evidence of protection against the FB 1 toxic effects by this detoxification procedure (p < 0.05).

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Development of Fumonisin-Induced Hepatotoxicity and Pulmonary Edema in Orally Dosed Swine: Morphological and Biochemical Alterations

Abstract: The fumonisin (FB) mycotoxins induce liver injury in all species but induce fatal pulmonary edema (PE)

Cited by 61 publications

References 28 publications

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Mycotoxin Fumonisin B ₁ Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs

Glucose Reaction with Fumonisin B₁Partially Reduces Its Toxicity in Swine

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Development of Fumonisin-Induced Hepatotoxicity and Pulmonary Edema in Orally Dosed Swine: Morphological and Biochemical Alterations

Abstract: The fumonisin (FB) mycotoxins induce liver injury in all species but induce fatal pulmonary edema (PE)

Cited by 61 publications

References 28 publications

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Fumonisin B−Glucose Reaction Products Are Less Toxic When Fed to Swine

Mycotoxin Fumonisin B 1 Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs

Glucose Reaction with Fumonisin B1Partially Reduces Its Toxicity in Swine

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Product

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Mycotoxin Fumonisin B ₁ Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs

Glucose Reaction with Fumonisin B₁Partially Reduces Its Toxicity in Swine