Development of fly tolerance to consuming a high-protein diet requires physiological, metabolic and transcriptional changes

Yurkevych, Ihor S.; Gray, Lindsey J.; Gospodaryov, Dmytro V.; Burdylyuk, Nadia; Storey, Kenneth B.; Simpson, Stephen J.; Lushchak, Oleh

doi:10.1007/s10522-020-09880-0

Cited by 7 publications

(10 citation statements)

References 78 publications

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“…In addition, TotA expression is induced by various environmental stresses such as UV light, heat, cold, bacterial infection, and oxidative agents . Moreover, TotA expression is also increased when flies adapt to a high-protein-low-carbohydrate diet . It is unclear why a broad range of stress stimuli increases TotA transcription while lipid unsaturation decreases TotA protein levels.…”

Section: Discussionmentioning

confidence: 99%

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Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye

Kumar,

Has,

Lam-Kamath

et al. 2024

J. Proteome Res.

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Organisms respond to dietary and environmental challenges by altering the molecular composition of their glycerolipids and glycerophospholipids (GPLs), which may favorably adjust the physicochemical properties of lipid membranes. However, how lipidome changes affect the membrane proteome and, eventually, the physiology of specific organs is an open question. We addressed this issue in Drosophila melanogaster, which is not able to synthesize sterols and polyunsaturated fatty acids but can acquire them from food. We developed a series of semisynthetic foods to manipulate the length and unsaturation of fatty acid moieties in GPLs and singled out proteins whose abundance is specifically affected by membrane lipid unsaturation in the Drosophila eye. Unexpectedly, we identified a group of proteins that have muscle-related functions and increased their abundances under unsaturated eye lipidome conditions. In contrast, the abundance of two stress response proteins, Turandot A and Smg5, is decreased by lipid unsaturation. Our findings could guide the genetic dissection of homeostatic mechanisms that maintain visual function when the eye is exposed to environmental and dietary challenges.

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Section: Discussionmentioning

confidence: 99%

“… 28 Moreover, TotA expression is also increased when flies adapt to a high-protein-low-carbohydrate diet. 45 It is unclear why a broad range of stress stimuli increases TotA transcription while lipid unsaturation decreases TotA protein levels. Yet, in another study, a high-fat diet caused TotA downregulation specifically in males.…”

Section: Discussionmentioning

confidence: 99%

Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye

Kumar,

Has,

Lam-Kamath

et al. 2024

J. Proteome Res.

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“…Determination of enzymatic activities. Activities of malate dehydrogenase (MDH, EC 1.1.1.37), lactate dehydrogenase (LDH, EC 1.1.1.27), alanine transaminase (ALT, EC 2.6.1.2), and aspartate transaminase (AST, EC 2.6.1.1) were determined by assaying the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time [3,18]. The ALT and AST activities were determined by using the commercial diagnostic kits (PZ Cormay S.A., Poland).…”

Section: Methodsmentioning

confidence: 99%

“…The method for urea determination was based on the monitoring of concentration NADH oxidized by glutamate dehydrogenase coupled to urease reaction where ammonia was formed from urea. Urea content was expressed as nanogram per milligram wet mass (ng/mgwm) [3,18]. Change in absorbance was recorded on Specol 211 (Jena, Germany) at 340 nm and calculation was made using an extinction coefficient of 6220 М -1 •cm -1 .…”

Section: Fig 1 Experimental Design Metabolic Enzymes Activity and Urea Content Were Investigated In Drosophila Melanogaster Of Parental Amentioning

confidence: 99%

Yeast concentration in the diet defines Drosophila metabolism of both parental and offspring generations

Strilbytska¹,

Stefanyshyn²,

Semaniuk³

et al. 2021

Ukr.Biochem.J.

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Parental dietary nutrients epigenetically influence offspring metabolism. Our analysis revealed unforeseen patterns in how enzymes of the main metabolic pathways respond to protein content in the diet. We reared parental flies Drosophila melanogaster on four types of diet with different dry yeast concentrations ranging from 0.25% to 15%. the subsequent generation was fed by the same diet, so the only variable in the experiments was the yeast concentration in the parental diet. We showed that protein restriction in the parental diet led to higher lactate dehydrogenase (LDH) activity in parents, and this effect was inherited in their progeny. The transgenerational effect of parental dietary yeast on malate dehydrogenase (MDH) activity was found only in males. An elevated level of dietary yeast was sufficient to enhance alanine transaminase ( ALT) and aspartate transaminase (AST) activity in parents, however, did not affect ALT activity and decreased AST in their offspring. A low yeast parental diet was shown to cause higher urea content in F1 males. It is concluded that parental dietary yeast plays a critical role in metabolic health that can be inherited through generation.

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“…Calculation of urea content was carried out by using an extinction coefficient of 6220 mol/L À1 /cm. Metabolite content is expressed as nanogram per milligram of wet mass (ng/mg wm) (Yurkevych et al 2020).…”

Section: Determination Of Ureamentioning

confidence: 99%

Parental dietary sucrose affects metabolic and antioxidant enzyme activities in Drosophila

Strilbytska

Strutynska

Semaniuk

et al. 2021

Entomological Science

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The influence of parental nutrition as a key environmental factor that can influence offspring performance has become a hot topic for modern investigations. In the current study using fruit flies, Drosophila melanogaster, we found the sucrose concentration of the diet in the parental generation had a significant influence on the metabolism and antioxidant defense of the next generation, even when that subsequent generation had been shifted to a standard diet. We found that low concentrations of dietary carbohydrate in the parental generation led to higher activities of lactate dehydrogenase, malate dehydrogenase (MDH) and alanine transaminase (ALT) enzymes and increased urea content in their progeny. Moreover, higher activities of the firstand second-line antioxidant defense enzymes were detected in F1 males generated from parents fed a low-carbohydrate diet. Low sucrose concentration in the parental diet resulted in increased levels of protein thiols but decreased the low-molecular-weight thiol group content in F1 males. The influence of different dietary sucrose concentrations also resulted in sex-dependent differences, where MDH, ALT, glutathione-S-transferase, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activities and levels of thiol groups were unaffected in F1 females. The results highlight the transgenerational influence that specific dietary macronutrients provided in the parental diet, namely carbohydrates, can have on the next generation, influencing enzyme activities, metabolic pathways and antioxidant defenses in progeny Drosophila.

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Development of fly tolerance to consuming a high-protein diet requires physiological, metabolic and transcriptional changes

Cited by 7 publications

References 78 publications

Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye

Lipidome Unsaturation Affects the Morphology and Proteome of the Drosophila Eye

Yeast concentration in the diet defines Drosophila metabolism of both parental and offspring generations

Parental dietary sucrose affects metabolic and antioxidant enzyme activities in Drosophila

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