Development of drug delivery systems for the dermal application of therapeutic DNAzymes

Schmidts, Thomas; Marquardt, Kay; Schlupp, Peggy; Dobler, Dorota; Heinz, Florian; Mäder, Ulf; Garn, Holger; Renz, Harald; Zeitvogel, Jana; Werfel, Thomas; Runkel, Frank

doi:10.1016/j.ijpharm.2012.04.034

Cited by 21 publications

(9 citation statements)

References 43 publications

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“…Skin conditions can be influenced by skin diseases and often are attended by disruption of the stratum corneum. Therefore, it is essential to consider skin condition in the early stage of dermal drug formulation development because of possible modified drug transport into and across the skin [28]. To determine the drug levels within the stratum corneum, tape stripping is a well-established method [29,30].…”

Section: Resultsmentioning

confidence: 99%

Development and validation of an alternative disturbed skin model by mechanical abrasion to study drug penetration

Schlupp

Weber

Schmidts

et al. 2014

Results in Pharma Sciences

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Pharmaceuticals and cosmetics for dermal application are usually tested on healthy skin, although the primary permeation barrier, the stratum corneum, is often impaired by skin diseases or small skin lesions, especially on the hands. These skin conditions can considerably influence the permeation of chemicals and drugs. Furthermore, risk assessment for example of nanoparticles should be performed under various skin conditions to reflect the true circumstances. Therefore, an alternative and reproducible method for a high throughput of skin samples with impaired skin barrier was developed and verified by skin permeation studies (25 h) of caffeine, sorbic acid and testosterone compared to healthy (untreated) and tape-stripped skin. Skin barrier disruption was controlled by TEWL measurement.Skin permeation of the three substances was increased in tape-stripped and abraded skin compared to untreated skin due to the reduced barrier integrity. Enhancement of drug uptake was highest for the most hydrophilic substance, caffeine, followed by sorbic acid and lipophilic testosterone. No significant difference in drug uptake studies was observed between the new abrasion method with an aluminum-coated sponge and the tape-stripping method. The obtained results demonstrate that this abrasion method is an alternative way to achieve a disturbed skin barrier for drug and chemical uptake studies.

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Section: Resultsmentioning

confidence: 99%

Development and validation of an alternative disturbed skin model by mechanical abrasion to study drug penetration

Schlupp

Weber

Schmidts

et al. 2014

Results in Pharma Sciences

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“…In contrast, microscopic evaluation of vertical slices has the capability to show profiles directly throughout the whole skin, but lacks the possibility to quantify the amount of penetrated substances. Schmidts 30 presented a skin penetration study that correlated an ELISA based quantification with an intensity-based microscopy evaluation method. The results were of good qualitative accordance indicating the suitability of the microscopy approach for evaluating dermal delivery systems.…”

Section: Pharmaceutical In Vitro Experimentsmentioning

confidence: 99%

Evaluation and quantification of spectral information in tissue by confocal microscopy

Maeder¹,

Marquardt²,

Beer³

et al. 2012

J. Biomed. Opt

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Abstract. A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.

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“…Multiple water‐in‐oil‐in‐water (W/O/W) emulsions are of great interest as potential drug delivery systems of hydrophilic drugs, oligonucleotides or vaccines due to a possible entrapment of hydrophilic substances into the inner water droplets [1–3]. While the use of such double emulsions is widely investigated for oral administration of drugs, in the food industry and in cosmetics only a few studies deal with multiple emulsions as potential skin delivery systems for hydrophilic drugs [1,2,4–8]…”

Section: Introductionmentioning

confidence: 99%

“…Multiple water-in-oil-in-water (W/O/W) emulsions are of great interest as potential drug delivery systems of hydrophilic drugs, oligonucleotides or vaccines due to a possible entrapment of hydrophilic substances into the inner water droplets. [1][2][3] While the use of such double emulsions is widely investigated for oral administration of drugs, in the food industry and in cosmetics only a few studies deal with multiple emulsions as potential skin delivery systems for hydrophilic drugs. [1,2,[4][5][6][7][8] With the use of multiple W/O/W emulsions, we can expect two principal advantages: (1) hydrophilic drugs incorporated in aqueous droplets will be protected by a hydrophobic layer; and (2) due to this protection, these formulations should influence the skin distribution of actives after topical application.…”

Section: Introductionmentioning

confidence: 99%

“…[1,2,[4][5][6][7][8] With the use of multiple W/O/W emulsions, we can expect two principal advantages: (1) hydrophilic drugs incorporated in aqueous droplets will be protected by a hydrophobic layer; and (2) due to this protection, these formulations should influence the skin distribution of actives after topical application. [2,9] To maintain these advantages, the preservation of the microstructure is of great importance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Natural polymer-stabilized multiple water-in-oil-in-water emulsions: a novel dermal drug delivery system for 5-fluorouracil

Hoppel

Mahrhauser

Stallinger

et al. 2013

Journal of Pharmacy and Pharmacology

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Development of drug delivery systems for the dermal application of therapeutic DNAzymes

Cited by 21 publications

References 43 publications

Development and validation of an alternative disturbed skin model by mechanical abrasion to study drug penetration

Development and validation of an alternative disturbed skin model by mechanical abrasion to study drug penetration

Evaluation and quantification of spectral information in tissue by confocal microscopy

Natural polymer-stabilized multiple water-in-oil-in-water emulsions: a novel dermal drug delivery system for 5-fluorouracil

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