Development and validation of an alternative disturbed skin model by mechanical abrasion to study drug penetration

Abstract

Pharmaceuticals and cosmetics for dermal application are usually tested on healthy skin, although the primary permeation barrier, the stratum corneum, is often impaired by skin diseases or small skin lesions, especially on the hands. These skin conditions can considerably influence the permeation of chemicals and drugs. Furthermore, risk assessment for example of nanoparticles should be performed under various skin conditions to reflect the true circumstances. Therefore, an alternative and reproducible method for a high throughput of skin samples with impaired skin barrier was developed and verified by skin permeation studies (25 h) of caffeine, sorbic acid and testosterone compared to healthy (untreated) and tape-stripped skin. Skin barrier disruption was controlled by TEWL measurement.Skin permeation of the three substances was increased in tape-stripped and abraded skin compared to untreated skin due to the reduced barrier integrity. Enhancement of drug uptake was highest for the most hydrophilic substance, caffeine, followed by sorbic acid and lipophilic testosterone. No significant difference in drug uptake studies was observed between the new abrasion method with an aluminum-coated sponge and the tape-stripping method. The obtained results demonstrate that this abrasion method is an alternative way to achieve a disturbed skin barrier for drug and chemical uptake studies.

Development of haemostatic decontaminants for treatment of wounds contaminated with chemical warfare agents. 3: Evaluation of in vitro topical decontamination efficacy using damaged skin

“…While the effects of damage, such as needle punctures, abrasion or tape stripping, on general skin permeability have been assessed previously in vitro (Chilcott et al, ; Davies et al, ; Schlupp et al, ; Wu et al, ), to our knowledge this study is the first to use in vitro static diffusion cells to assess the penetration and decontamination of CW agents through damaged skin. Skin damage (removal of upper 100 μm layer) resulted in an increase in the amount of 14 C–HD and 14 C–GD detected in the receptor fluid compared to undamaged skin.…”

Epicutaneous administration of the pattern recognition receptor agonist polyinosinic‐polycytidylic acid activates the MDA5/MAVS pathway in Langerhans cells

“…The potential of a topically applied synthetic RNA to induce activation of LCs and KCs in their natural environment was studied using an ex vivo barrier-disrupted human skin explant culture model. Removal of the stratum corneum, the outmost epidermal layer, by mechanical abrasion such as tape stripping, is a widely used method of barrier perturbation (39). Indeed, examination of hematoxylin and eosinstained sections showed that compared to untreated skin, 50 repetitions of adhesive tape stripping largely removed the stratum corneum without disrupting the integrity of the underlying skin architecture (Fig.…”

Hydrophilic Ionic Liquids as Ingredients of Gel-Based Dermal Formulations

“…The skin diffusion area was 1.76 cm 2 . These experimental conditions have been previously verified (24).…”

“…The equipment was operated at 40°C. The methods for HPLC analysis of caffeine and testosterone have been described in detail elsewhere (24).…”