Development of calcium phosphate coatings with regulated porous structure as drug carrier systems

Abstract

The calcium phosphate coatings were formed by the micro-arc oxidation method under different applied voltage. It was shown that the thickness, surface roughness, porosity and sizes of the structural elements of the coatings increased with increasing of the applied voltage. In addition, the increase in the voltage led to the structural-phase transformation in the coatings from the amorphous state to the amorphous-crystalline state with incorporation of CaHPO4, α-Ca2P2O7, β-Ca2P2O7 and TiO2 (anatase) nano-sized phases. The micro-porous amorphous coatings formed at a low voltage of 200 V showed the highest adsorption of the doxorubicin. The formed coatings with regulated porous structure and specified phase composition can be used as anticancer (e.g., doxorubicin) drug carrier systems.

Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

“…The unipolar anodic potentiostatic regime at a fixed pulse frequency of 50 Hz, pulse duration of 100 µs, time of 10 min, and varied voltage from 150 to 300 V with a step of 50 V was used as previously described [ 30 ].…”

Effect of the Porosity, Roughness, Wettability, and Charge of Micro-Arc Coatings on the Efficiency of Doxorubicin Delivery and Suppression of Cancer Cells

“…It is known that the surface morphology and developed relief play a significant role in the interaction of CaP coatings with biological fluids and living systems (cells, bacteria) [37] and when they are used as carriers of drugs. In this case, the porous structure of the coatings plays a special role, since the nature of the porosity, the size, and the pore shape have a significant effect on the processes of drug adsorption and its controlled release [38]. Based on the obtained data, the calibration graph of the dependence of optical density on the number of living cells in the cell suspension (Figure 2b) was plotted.…”

“…It is known that the surface morphology and developed relief play a significant role in the interaction of CaP coatings with biological fluids and living systems (cells, bacteria) [37] and when they are used as carriers of drugs. In this case, the porous structure of the coatings plays a special role, since the nature of the porosity, the size, and the pore shape have a significant effect on the processes of drug adsorption and its controlled release [38]. The SEM studies demonstrated that CaP coatings with different surface morphologies were formed depending on the applied voltage (Figure 4a-c An increase in the applied voltage from 250 to 300 V led to an increase in the intensity of microarc discharges acting on the substrate; as a result, the surface morphology of the coatings changed.…”

VLIYaNIE IERARKhII POROVOY STRUKTURY NA FIZIKO-MEKhANIChESKIE SVOYSTVA KAL''TsIY-FOSFATNYKh NOSITELEY LEKARSTVENNYKh SREDSTV

“…Разработаны и изучены объемные макропористые кальций-фосфатные (КФ) покрытия на титановых матрицах в качестве носителей лекарственных средств, в частности, противоопухолевых препаратов [1]. Синтез КФ покрытий на образцы из технически чистого титана марки ВТ1-0 проводили методом микродугового оксидирования (МДО) при варьировании величины приложенного импульсного напряжения в интервале 150 -350 В. Остальные электрофизические параметры МДО были фиксированными: длительность импульсов тока -100 мкс; частота импульсов тока -50 Гц; форма импульсовпрямоугольная; коэффициент заполнения -0,5 %; длительность процесса МДО -10 мин.…”

“…Верхний (поверхностный) КФ слой представлен сфероидальными структурными элементами (сферы, полусферы), имеющими размеры 4-50 мкм, в которых содержатся внутренние поры с размерами 1-20 мкм, также на поверхности присутствуют поры, находящиеся в «нишах» между сфероидальными образованиями. Как показали предыдущие исследования [1,2], основной и поверхностный слои покрытия находятся преимущественно в аморфнонанокристаллическом состоянии, состоящие из аморфного КФ, а также нанокристаллических фаз CaHPO 4 и β-Ca 2 P 2 O 7 .…”