Development of bactericidal properties against gram-negative organisms in the serum of young animals

Šterezl, J.; Kostka, J.; Lanc, A

doi:10.1007/bf02928237

Cited by 50 publications

(16 citation statements)

References 47 publications

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“…Serum obtained from several mammalian species during early postnatal development has been reported to lack significant bactericidal activity against smooth strains of enteric bacilli (2,18). In other studies, serum from 3to 4-week-old rabbits, guinea pigs, and rats was reported to be essentially nonbactericidal for certain gram-negative bacteria (9,10).…”

Section: Tsamentioning

confidence: 99%

Humoral bactericidal systems: antibacterial potential of serum from young animals

Skarnes¹

1978

Infect Immun

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The antibacterial potential of fresh serum obtained from young animals during a pre-antibody period of development was assessed against two smooth and two rough strains of gram-negative bacteria. The bactericidal capacity of serum from 3- to 4-week-old guinea pigs and 4- to 5-week-old rabbits was compared with that of serum from adults. Serum from young animals was deficient in natural antibodies, and in conventional dilution assays the bactericidal action was unimpressive, especially against the smooth strains. However, when decimal increments of bacteria were incubated in fresh undiluted serum, killing of both smooth and rough strains proved substantial. This finding may have particular meaning in the very young animal when natural antibodies are at ebb: cellular defense mechanisms may function less efficiently at this time and effect a greater reliance on humoral antibacterial systems.

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Section: Tsamentioning

confidence: 99%

Humoral bactericidal systems: antibacterial potential of serum from young animals

Skarnes¹

1978

Infect Immun

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“…An area of controversy, however, is the question of antibody involvement. Some authors have suggested that in certain circumstances, complement, with or without lysozyme, may constitute a" complete bactericidal or bacteriolytic system (Sterzl et al 1962;Wardlaw, 1962;Dlaba;1968;Barta et al 1971) whereas others have stated that antibody is always required before compleCan. J. Microbiol.…”

Section: Discussionmentioning

confidence: 97%

Bacteriolytic and bactericidal activity of maternal and cord sera. Relationship to complement, lysozyme, transferrin, and immunoglobulin levels

Pruzanski¹,

Leers²,

Wardlaw³

1972

Can. J. Microbiol.

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Bacteriolytic activity and its relationship to the level of immunoglobulins, complement, lysozyme, and transferrin was measured in 20 pairs and bactericidal activity in 6 pairs of maternal and cord serum. Escherichia coli Lilly, an organism easily lysed by normal human serum and four other Gram-negative bacteria were used.Cord sera invariably had lower bacteriolytic and bactericidal activity than their corresponding maternal sera. No statistically significant relationship between these activities and the level of immunoglobulins, complement, lysozyme, or transferrin was detected. A higher level of lysozyme was unable to compensate for the lower levels of other factors in the cord sera.

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“…However, a trace amount of the antibody may exist in the normal human sera, because V. vulnificus is a normal habitant in estuarine or coastal areas and people in Japan eat seafoods very often. The amount of antibody required for complement activation in the classical pathway is extremely small (15,19,23). Taylor and Kroll (22) reported rapid killing of bacteria by antibody-dependent classical pathway in huma serum obtained from healthy donors.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Capsular Antigen of Vibrio vulnificus on Bactericidal Activity of Human Serum

Shinoda

Kobayashi

Yamada

et al. 1987

Microbiology and Immunology

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Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr variants of some strains, in which the difference of the sensitivity between Op and Tr cells was remarkable.Then agglutination tests with their living and heat-killed cells were carried out. The results suggested the presence of capsular antigen in Op cells and its absence in Tr cells, with the exception of the existence of a slight amount of capsular material in Tr variants of strain L-180.The thin capsular layer of Tr cells of strain L-180 was also demonstrated electron microscopically, but the layer was thinner than that of the isogenic Op cells. Results of determination of sugar content in the extracted capsular fraction also showed that Op to Tr transformation was due to loss of capsular antigen of the cells. These results confirmed the morphological studies previously reported which suggested the prevention of host defense system by the capsular material of the vibrio.Vibrio vulnificus, a slightly halophilic marine bacterium, has been recently recognized as a pathogen causing wound infection or septicemia (5,6,14). It has been noted that almost all the patients with septicemia have underlying diseases, such as hepatitis, alcoholism, or diabetes (3). The decrease of phagocytosis accompanying dyshepatia might therefore promote survival of the vibrio in the blood stream. Association of virulence of the organism with resistance to phagocytosis by human polymorphonuclear leukocytes has been reported (8). Vibrio vulnificus isolates from blood are more resistant to serum bactericidal activity and phagocytosis than isolates from other sources (4, 7) ; V. vulnificus is a weak activator of complement and resists phagocytosis to a greater degree than other vibrios, such as V. cholerae or V. parahaemolyticus (20,21). Amako et al (1) and Yoshida et al (25) showed the existence of capsule-like material in opaque-colony-forming cells of V. vulnificus by electron microscopy and suggested the relationship between the capsule-like material and the virulence of the bacterium. Kreger et al (9) also reported that acidic poly-393

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Development of bactericidal properties against gram-negative organisms in the serum of young animals

Cited by 50 publications

References 47 publications

Humoral bactericidal systems: antibacterial potential of serum from young animals

Humoral bactericidal systems: antibacterial potential of serum from young animals

Bacteriolytic and bactericidal activity of maternal and cord sera. Relationship to complement, lysozyme, transferrin, and immunoglobulin levels

Inhibitory Effect of Capsular Antigen of Vibrio vulnificus on Bactericidal Activity of Human Serum

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