Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells

Kaur, P.; Robin, .; Mehta, Rajendra G.; Singh, Balbir; Arora, Saroj

doi:10.1186/s12906-019-2432-9

Cited by 26 publications

(18 citation statements)

References 41 publications

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“…The extraction of polyphenols from plant material requires the use of aqueous mixtures of organic solvents such as acetone, acetonitrile, methanol, and ethanol [29, 30]. Ethanol is deemed the best solvent to extract polyphenols, and it is safe for human consumption.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the in vitro effects of commercial herbal preparations significant in African traditional medicine on platelets

Mothibe

Kahler-Venter

Osuch

2019

BMC Complement Altern Med

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Background Commercial herbal medicines (CHMs) marketed as immune boosters are gaining wide popularity in South Africa, in the absence of control and regulatory guidelines. These commercially packaged and labelled herbal preparations, acquired in various retail outlets, are used without consulting either a conventional health provider or a traditional health practitioner. Although they are indicated for immune-boosting purposes, they might exert many other beneficial and unwanted effects on physiological systems. Platelets are crucial in haemostasis and important for the immunological system. The aim was to investigate the effect of the CHMs used to strengthen the immune system on the activity of human platelets. Methods Six CHMs commonly used as African traditional medicines in Pretoria, South Africa, were tested for their effects on healthy, isolated human platelets, using a bioluminescence method. The tested herbal medicines were Intlamba Zifo™ , Maphilisa ™ Herbal medicine, Matla ™ African medicine for all diseases, Ngoma ™ Herbal Tonic Immune Booster, Stametta ™ Body Healing Liquid, and Vuka Uphile ™ Immune Booster and serial-diluted standards of each from 10 to 10,000 times. The luminol-enhanced luminescence activity of the platelets was measured after incubation with the herbal medicines and activation with phorbol myristate acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanine (fMLP). Results Five herbal medicines, namely Intlamba Zifo™ , Maphilisa ™ Herbal medicine, Matla ™ African medicine for all diseases, Stametta ™ Body Healing Liquid, and Vuka Uphile ™ Immune Booster exerted comparable weak inhibitory effects on both PMA and fMLP-induced platelets, which were concentration dependent at high doses, and inversely related to concentration at low doses. Intlamba Zifo™ , Matla ™ African medicine for all diseases, Stametta ™ Body Healing Liquid, and Vuka Uphile ™ exhibited weak, but non-systematic stimulatory effects at low doses, which were not statistically significant. Ngoma ™ Herbal Tonic Immune Booster had weak, inhibitory effects at high doses and weak stimulatory effects that were inversely related to concentration at low doses. Conclusion The findings suggest a potential beneficial role of the CHMs in the suppression of platelets’ reactivity and in enhancing the immune system. Caution, however, should be exercised as platelet inhibition and stimulation predispose to the risk of bleeding and thrombosis, respectively.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the in vitro effects of commercial herbal preparations significant in African traditional medicine on platelets

Mothibe

Kahler-Venter

Osuch

2019

BMC Complement Altern Med

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“…Resazurin assay was also used to detect ESC proliferation according to previous report [20]. ESCs (2 × 10 4 ) were seeded into a 96-well plate and incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

LncRNA H19 over-expression inhibited Th17 cell differentiation to relieve endometriosis through miR-342-3p/IER3 pathway

Liu

Zhong

et al. 2019

Cell Biosci

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Objective To investigate the mechanism of LncRNA H19 in Th17 cell differentiation and endometrial stromal cells (ESCs) proliferation in endometriosis (EMS). Methods LncRNA H19, miR-342-3p and IER3 expressions were detected by qRT-PCR and western blot. The percentage of Th17 cells/CD4+ T cells was detected by flow cytometry. IL-17 level was measured by ELISA. The interaction of miR-342-3p and IER3 was confirmed by Luciferase reporter assay. Results LncRNA H19 and IER3 expressions were down-regulated in mononuclear cells from peritoneal fluid (PFMCs) of patients with EMS or under Th17 differentiation conditions, whereas miR-342-3p expression was up-regulated and the percentage of Th17 cells was increased in PFMCs of patients with EMS or under Th17 differentiation conditions. Over-expression of LncRNA H19 decreased IL-17 level and the percentage of Th17 cells/CD4+ T cells. Besides, we confirmed that miR-342-3p could target to IER3 and negatively regulate IER3 expression. LncRNA H19 over-expression suppressed Th17 differentiation and ESC proliferation through regulating miR-342-3p/IER3. In vivo experiments showed LncRNA H19 over-expression suppressed the growth of Th17 cell differentiation-induced endometriosis-like lesions. Conclusion LncRNA H19 was down-regulated in PFMC of patients with EMS or under Th17 polarizing conditions, and LncRNA H19 over-expression suppressed Th17 cell differentiation and ESCs proliferation through miR-342-3p/IER3 pathway.

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“…This reaction was observed under UV-VIS spectrophotometer (UV-2800) at 517 nm. Absorbance values at the time of reaction were noted and used to calculate percentage anti-oxidant activity (%AA) as previously described [18,19].…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

Lirioresinol B dimethyl ether inhibits NF-κB and COX-2 and activates IκBα expression in CCl4-induced hepatic fibrosis

Shehzad

Rehmat

Ul-Islam

et al. 2020

BMC Complement Med Ther

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Background: Inflammation is one of the key components in the initiation and progression of hepatic diseases. If not treated, inflammation may cause cell dysplasia, and ultimately cancer. In the current study, we investigated the anti-inflammatory and anti-cancer activities of plant isolated compound Lirioresinol B Dimethyl Ether (LBDE) extracted from the seeds of Magnolia fargesii CHENG (Magnoliaceae) against HepG2 cells as well as in BALB/C male mice. Methods: We assessed the antioxidant and anti-proliferative effects of plant compounds using DPPH assay and HepG2 cell lines. Carbon tetrachloride (CCl 4 ) and Diethylnitrosamine (DEN) were used to induce liver cell dysplasia followed by hepatocellular carcinoma (HCC) in BALB/C male mice for 12 weeks. We investigated the underlying mechanism by using histopathology and immunoblot experiments.Results: Intraperitoneal injection of LBDE (50 mg/kg body weight/day) inhibited CCl 4 -induced HCC. Free radical scavenging assay shows the strong anti-oxidant activity of LBDE. Western blot results show that LBDE downregulated nuclear factor kappa B (NFκB) and cyclooxygenase (COX-2) by preventing the phosphorylation of I kappa B alpha (IκBα) in CCl 4 treated group. LBDE also improved liver function by decreasing Alkaline Phosphatase (ALP), aspartate aminotransferase (AST) and Alanine Aminotransferase (ALT) levels. Histopathology results revealed that LBDE decreased granulomas and express normal morphology of hepatocytes.Conclusions: These preliminary results show that LBDE has the potential to inhibit CCl 4 -induced liver cell dysplasia and prevents cancer development by regulating NFκB/COX-2 activation.

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Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells

Cited by 26 publications

References 41 publications

Evaluation of the in vitro effects of commercial herbal preparations significant in African traditional medicine on platelets

Evaluation of the in vitro effects of commercial herbal preparations significant in African traditional medicine on platelets

LncRNA H19 over-expression inhibited Th17 cell differentiation to relieve endometriosis through miR-342-3p/IER3 pathway

Lirioresinol B dimethyl ether inhibits NF-κB and COX-2 and activates IκBα expression in CCl4-induced hepatic fibrosis

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