Development of an on-line isotope dilution technique with HPLC/ICP-MS for the accurate determination of elemental species

Rottmann, Lothar; Heumann, Klaus G.

doi:10.1007/bf00322473

Cited by 143 publications

(139 citation statements)

References 27 publications

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“…The application of isotope-dilution analysis to ICP-MSbased hyphenated techniques, for example HPLC-ICP-MS, can be divided into two different modes: the species-unspecific mode and species-specific mode (Rottmann & Heumann, 1994;Rodriguez-Gonzalez et al, 2005). The two modes are illustrated in Figure 1.…”

Section: Isotope Dilution Analysis (Ida) By Icp-msmentioning

confidence: 99%

ICP‐MS‐Based strategies for protein quantification

Wang

Feng

Zhao

et al. 2009

Mass Spectrometry Reviews

105

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In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasma-mass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MS-based technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.

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Section: Isotope Dilution Analysis (Ida) By Icp-msmentioning

confidence: 99%

ICP‐MS‐Based strategies for protein quantification

Wang

Feng

Zhao

et al. 2009

Mass Spectrometry Reviews

105

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“…9 The species-specific isotope dilution modality SSID search for quantification of the species itself and is performed by spiking a sample with the isotopic enriched labeled species. Nevertheless, this case is out of the scope of the present review once the isotopic labeling is performed offline, commonly during sample preparation and derivatization.…”

Section: Id Modes For Speciation Analysismentioning

confidence: 99%

“…However, the application of online ID for speciation analysis by ICP-MS has been largely applied since the proposal of Rottman and Heumann in 1994. 9 Authors described the online species-unspecific quantification by ID of one or more elements in different species by sample and spike mass flow calibration, regardless of their chemical forms.…”

Section: Introductionmentioning

confidence: 99%

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

Giné¹,

Packer²

2010

J. Braz. Chem. Soc.

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Apresentam-se sistemas de análises em fluxo propostos para a quantificação de elementos e espécies por diluição isotópica (ID) utilizando espectrometria de massas com fonte de plasma acoplada indutivamente (ICP-MS). Detalham-se os esquemas para adicionar em linha a solução isotopicamente enriquecida à amostra. São relatadas as aplicações em meio aquoso e outras com transferência do analito para a fase vapor, seja por processo de vaporização eletrotérmica, geração de vapor ou ablação por laser. Apresentam-se as aplicações com quantificação de espécies por ID caracterizadas pela adição do isótopo enriquecido, antes ou após a separação das espécies, seja por cromatografia a líquido, a gás ou por eletroforese capilar acoplada a ICP-MS. As principais equações envolvidas na quantificação por ID e as principais fontes de erro são descritas. São discutidas as mudanças introduzidas na estratégia de quantificação temporal por ID de elementos e espécies pela calibração do fluxo de massas da amostra e do isótopo enriquecido adicionado.Flow analysis has been utilized for elemental and species quantification by online isotope dilution inductively coupled plasma mass spectrometry (ICP-MS). Online isotope dilution (ID) herein refers to strategies promoting the addition of enriched isotopic solutions to the samples while flowing towards the plasma. Applications of online ID's are commonly performed in aqueous media, however, in some processes the analyte transference to the vapor phase using electrothermal vaporization, vapor generation or laser ablation is also required. Flow diagrams to perform online pre or post-column spiking with the enriched isotope for quantification by ID in speciation analysis by liquid chromatography, gas chromatography or capillary electrophoresis devices coupled to ICP-MS are presented. The main figures of merit and equations applied to elemental and species quantification by ID in flowing schemes are assessed.

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“…99,179 Over the last few years Heumann and co-workers have promoted the application of isotope-dilution approaches for accurate quantitative elemental speciation analysis using either non-speciesspecific or species-specific strategies. 108,[180][181][182] Based on this development, Prange and Schaumlöffel et al pioneered the field of sulfur isotopedilution analysis by combining postcolumn non-species-specific isotope dilution and capillary electrophoresis hyphenated to sector field ICP-MS for the highly resolved separation of different metallothionein (MT) isoforms, which allowed determination of the metal stoichiometry of the different isoforms as well as their absolute quantification. 183,184 A non-species-specific element spike containing 34 S, 65 Cu, 68 Zn, and 116 Cd introduced via the make-up flow of the CE interface was used for on-line isotope ratio measurement of 32 S/ 34 S, 63 Cu/ 65 Cu, 64 Zn/ 68 Zn, and 114 Cd/ 116 Cd.…”

mentioning

confidence: 99%

Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) for Quantitative Analysis in Environmental and Life Sciences: A Review of Challenges, Solutions, and Trends

2012

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This focal point review provides an overview of recent developments and capabilities of inductively coupled plasma mass spectrometry (ICP-MS) coupled with different separation techniques for applications in the fields of quantitative environmental and bio-analysis. Over the past years numerous technical improvements, which are highlighted in this review, have helped to promote the evolution of ICP-MS to one of the most versatile tools for elemental quantification. In particular, the benefits and possibilities of using state-of-the-art hyphenated ICP-MS approaches for quantitative analysis are demonstrated with a focus on environmental and bio-analytical applications.

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Development of an on-line isotope dilution technique with HPLC/ICP-MS for the accurate determination of elemental species

Cited by 143 publications

References 27 publications

ICP‐MS‐Based strategies for protein quantification

ICP‐MS‐Based strategies for protein quantification

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) for Quantitative Analysis in Environmental and Life Sciences: A Review of Challenges, Solutions, and Trends

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