ICP‐MS‐Based strategies for protein quantification

Abstract

In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasma-mass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MS-based technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.

Phenol-oxidizing laccases from the termite gut☆

“…Inductively coupled plasma-mass spectrometry (ICP-MS; Wang et al, 2009) was conducted to determine metal ligand composition for the recombinant RfLacA protein. Prior to digestion, protein samples were dialyzed to remove unbound metals.…”

Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

“…Not only metal-containing biomolecules, but also other biomolecules, such as amino acids, peptides, proteins, nucleic acids and microbial cells, can be successfully quantified by measuring heteroatoms, such as phosphorus and sulfur, and/or by supporting elemental tags, such as metal-labeled antigens and nanoparticles. [4][5][6][7][8][9][10] In recent years, cytometric analysis of single cells with a time-resolved ICP-MS measurement is receiving much attention for elemental [14][15][16][17][18] and multiparametric [19][20][21][22][23] analyses of single cells. Due to cellular heterogeneity within an isogenic cell population, individual analysis of cells will lead to a more sensitive representation of cell-to-cell variations, instead of a stochastic average analysis by bulk measurements.…”

Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry : Part II. Applications

“…However, CYP2E1 levels appear to be modulated to some extent by the compounds So far, the previous applications have been related to the labelling of antibodies used for immune-reactions, but in the following example it should be demonstrated that this procedure can also be applied for proteins directly, as it was discussed in a review article recently. 370 Ahrends et al 371,372 used metal-coded affinity tags (MeCAT) for direct labelling of standard proteins and eye lens proteins for quantitative ICP-SFMS. As MeCAT reagents they chose derivatives of the lanthanide chelating DOTA to which a cysteine reactive maleimide moiety was attached via a spacer.…”