Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

Miyashita, Shin-ichi; Groombridge, Alexander S.; Fujii, Shin-ichiro; Takatsu, Akiko; Chiba, Koichi; Inagaki, Kenji

doi:10.2116/analsci.30.219

Cited by 29 publications

(20 citation statements)

References 47 publications

(93 reference statements)

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“…The quantification of iron load internalized into BM-MNC by NIRF, MRI, and ICP-MS (Table 2) showed signal detection according to the signal detection limitation of each technique as a function of their sensibility, spatial, and temporal resolutions [17,46]. Some studies reported an internalization of 0.69 ± 0.08 [47]; 1.33 ± 0.2 [48]; 2.01 ± 0.10 pg [49] of nanoparticles per HSC.…”

Section: Discussionmentioning

confidence: 99%

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

et al. 2021

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This study proposes an innovative way to evaluate the homing and tracking of hematopoietic stem cells from young and old mice labeled with SPIONNIRF-Rh conjugated with two types of fluorophores (NIRF and Rhodamine), and their grafting by bioluminescence (BLI) in a bone marrow transplant (BMT) model. In an in vitro study, we isolated bone marrow mononuclear cells (BM-MNC) from young and old mice, and analyzed the physical–chemical characteristics of SPIONNIRF-Rh, their internalization, cell viability, and the iron quantification by NIRF, ICP-MS, and MRI. The in vivo study was performed in a BMT model to evaluate the homing, tracking, and grafting of young and old BM-MNC labeled with SPIONNIRF-Rh by NIRF and BLI, as well as the hematological reconstitution for 120 days. 5FU influenced the number of cells isolated mainly in young cells. SPIONNIRF-Rh had adequate characteristics for efficient internalization into BM-MNC. The iron load quantification by NIRF, ICP-MS, and MRI was in the order of 104 SPIONNIRF-Rh/BM-MNC. In the in vivo study, the acute NIRF evaluation showed higher signal intensity in the spinal cord and abdominal region, and the BLI evaluation allowed follow-up (11–120 days), achieving a peak of intensity at 30 days, which remained stable around 108 photons/s until the end. The hematologic evaluation showed similar behavior until 30 days and the histological results confirm that iron is present in almost all tissue evaluated. Our results on BM-MNC homing and tracking in the BMT model did not show a difference in migration or grafting of cells from young or old mice, with the hemogram analysis trending to differentiation towards the myeloid lineage in mice that received cells from old animals. The cell homing by NIRF and long term cell follow-up by BLI highlighted the relevance of the multimodal nanoparticles and combined techniques for evaluation.

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Section: Discussionmentioning

confidence: 99%

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

et al. 2021

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“…This limitation can be overcome in time-resolved ICP-MS for single-cell analysis (Bendall et al 2012). Here, a cell suspension is passed to a plasma torch via a nebulizer or microdroplet dispenser followed by the generation of the ion cloud, which can be analyzed as an individual signal with a high time resolution (Miyashita et al 2014). A schematic representation of time-resolved ICP-MS for elemental analysis of single-cell is given in Fig.…”

Section: Inductively Coupled Plasma Mass Spectrometry (Icp-ms)mentioning

confidence: 99%

“…The lower region of the plot (P3) indicates the presence of human CD4 + T cells in human CD4 + T gate. They (Miyashita et al 2014). (ii) Human CD4 + T cells (purity 97.2%) were labeled with Gd-DTPA-BMA, washed extensively, and administered intraperitoneally (2 Â 10 6 ) to BALB/c immunodeficient mice together with 3 Â 10 6 autologous PBMC.…”

Section: Inductively Coupled Plasma Mass Spectrometry (Icp-ms)mentioning

confidence: 99%

Mass Spectrometry for Single-Cell Analysis

Patel

Dutta

Lim

2020

Handbook of Single Cell Technologies

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Cells are the basic structural and functional units in biology that plays important roles in living organisms. It can be affected by several factors that influence their proliferation, differentiation, and metabolic activities and consequently causing the wide heterogeneity among each cell within the same individual. It has been noted that the chemical composition and concentration found in two homogeneous cells are also different. Therefore, accurate cell morphology and composition can be obtained by studying at the single-cell level in a particular microenvironment. Nucleic acids, proteins, trace elements, and small molecule metabolites are the important chemicals present in cells and play key roles in cellular processes. Various analytic techniques such as flow cytometry, fluorescence microscopy, Raman microscopy, and mass spectrometry are used for single-cell analysis. Each technique has own advantages and disadvantages. Among them, mass spectrometry (MS) technique has received a considerable amount of attention owing to its high susceptibility and superior potential for the

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“…In recent years, time-resolved ICP-MS, known as single-cell ICP-MS (SC-ICP-MS), has attracted a great deal of interest for multielemental analysis of single cells as an alternative of classical bulk analysis of large amounts of cells with a lysis, extraction, or digestion (Miyashita et al 2014a(Miyashita et al , 2017Mueller et al 2014;Wang et al 2017a). This technique is able to detect and quantify metallic elements in individual cells with high sensitivity, with the advantage of obtaining information about cellto-cell variations instead of the average information from large cell populations obtained by conventional bulk measurements.…”

Section: Time Resolved Icp-ms Analysismentioning

confidence: 99%

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

Jiménez-Lamana

Szpunar

Łobiński

2018

Advances in Experimental Medicine and Biology

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Single cells represent the basic building units of life, and thus their study is one the most important areas of research. However, classical analysis of biological cells eludes the investigation of cell-to-cell differences to obtain information about the intracellular distribution since it only provides information by averaging over a huge number of cells. For this reason, chemical analysis of single cells is an expanding area of research nowadays. In this context, metallomics research is going down to the single-cell level, where high-resolution high-sensitive analytical techniques are required. In this chapter, we present the latest developments and applications in the fields of single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS), mass cytometry, laser ablation (LA)-ICP-MS, nanoscale secondary ion mass spectrometry (nanoSIMS), and synchrotron X-ray fluorescence microscopy (SXRF) for single-cell analysis. Moreover, the capabilities and limitations of the current analytical techniques to unravel single-cell metabolomics as well as future perspectives in this field will be discussed.

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Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

Cited by 29 publications

References 47 publications

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

Multimodal Tracking of Hematopoietic Stem Cells from Young and Old Mice Labeled with Magnetic–Fluorescent Nanoparticles and Their Grafting by Bioluminescence in a Bone Marrow Transplant Model

Mass Spectrometry for Single-Cell Analysis

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

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