Development of an LC–MS/MS method for the determination of endogenous cortisol in hair using 13 C 3 -labeled cortisol as surrogate analyte

Binz, Tina M.; Braun, Ueli; Baumgartner, Markus R.; Kræmer, Thomas

doi:10.1016/j.jchromb.2016.07.041

Cited by 48 publications

(53 citation statements)

References 29 publications

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“…The hair in a 5 by 5 cm area on the neck was removed using electric clippers, dried, wrapped in tin foil and stored at room temperature until analysis. Liquid chromatography tandem massspectrometry (LC-MS/MS) was used for measurement of the hair cortisol concentration as described in detail (Binz et al, 2016). Data were tested for normality using the Kolmogorov-Smirnov test, and mean and standard deviation were calculated for normal data and the median for non-normal data.…”

Section: Animals Materials and Methodsmentioning

confidence: 99%

Hair cortisol concentration and adrenal gland weight in healthy and ill cows

Braun

Clavadetscher

Baumgartner

et al. 2017

SAT

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Section: Animals Materials and Methodsmentioning

confidence: 99%

Hair cortisol concentration and adrenal gland weight in healthy and ill cows

Braun

Clavadetscher

Baumgartner

et al. 2017

SAT

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“…For this study, a validation was used with authentic matrix (a homogenized nail pool).For the 6 analytes aldosterone, corticosterone, 11-deoxycortisol, 11deoxycorticosterone, 17-OHP and DHT the concentration in the nail pool was sufficiently lowso that spiking theauthentic matrix with authentic analytes could perforemd. For the analytes cortisone, cortisol, androstenedione, testosterone, DHEA and progesterone, the approach of using a surrogate analyte was applied as described previously for measuring cortisol in hair [18]. Six stable isotope-labeled standards were chosen as surrogate analytes ( 13 C3-cortisone, 13 C3-cortisol, 13 C3androstenedione, 13 C3-testosterone, 13 C3-DHEA and 13 C3-progesterone).…”

Section: Validationmentioning

confidence: 99%

Steroid profiling in nails using liquid chromatography-tandem mass spectrometry

et al. 2018

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The retrospective analysis of endogenous steroid hormones in nails can be used to elucidate endocrine diseases and thus help with their diagnosis and treatment. A liquid chromatographytandem mass spectrometry (LC-MS/MS) based method was developed for the simultaneous identification and quantification of 12 steroid hormones (aldosterone, cortisone, cortisol, corticosterone, 11deoxycortisol, androstenedione, testosterone, dehydroepiandrosterone (DHEA), 17-hydroxyprogesterone (17-OHP), dihydrotestosterone (DHT) and progesterone) in human fingernails. Steroid hormones were extracted from 0.5 mg -10 mg pulverized nail clippings by methanolic extraction, followed by a liquid-liquid extraction. The analysis was conducted with LC-MS/MS in electrospray ionization positive mode. The method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, matrix effect, recovery and robustness. It was successfully applied for steroid profiling in nails of mothers and their infants where cortisol, cortisone, testosterone, progesterone, androstenedione and 11-deoxycorticosterone could be detected. Furthermore, it could be shown that there is no significant difference in concentrations between left and right hand for cortisol, cortisone and progesterone. A positive linear correlation between cortisol and cortisone in nails was found. In conclusion, it could be shown that nails are a suitable matrix for the retrospective monitoring of cumulative steroid hormone levels.Abstract: The retrospective analysis of endogenous steroid hormones in nails can be used to elucidate endocrine diseases and thus help with their diagnosis and treatment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) based method was developed for the simultaneous identification and quantification of 12 steroid hormones (aldosterone, cortisone, cortisol, corticosterone, 11-deoxycortisol, androstenedione, 11-deoxycorticosterone, testosterone, dehydroepiandrosterone (DHEA), 17α-hydroxyprogesterone (17-OHP), dihydrotestosterone (DHT) and progesterone) in human fingernails. Steroid hormones were extracted from 0.5 mg -10 mg pulverized nail clippings by methanolic extraction, followed by a liquid-liquid extraction. The analysis was conducted with LC-MS/MS in electrospray ionization positive mode. The method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, matrix effect, recovery and robustness. It was successfully applied for steroid profiling in nails of mothers and their infants where cortisol, cortisone, testosterone, progesterone, androstenedione and 11-deoxycorticosterone could be detected. Furthermore, it could be shown that there is no significant difference in concentrations between left and right hand for cortisol, cortisone and progesterone. A positive linear correlation between cortisol and cortisone in nails was found. In conclusion, it could be shown that nails are a suitable matrix for the retrospective monitoring of cumula...

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“…These columns can separate very similar molecules with high efficiency, and normally operate under high pressure [3]. Binz et al [11] used two methods (cortisol/(13)C3-labeled cortisol) were validated in a concentration range up to 500 pg/mg and showed good linearity for both analytes (cortisol: R(2)=0.9995; (13)C3-cortisol R(2)=0.9992). Slight differences were observed for limit of detection (LOD) (0.2 pg/ mg/0.1 pg/mg) and limit of quantification (LOQ) (1 pg/mg/0.5 pg/mg).…”

Section: Liquid Chromatographymentioning

confidence: 99%

“…Recovery was good with 92.7%/87.3% (RSD 9.9%/6.2%) for QC low and 102.3%/82.1% (RSD 5.8%/11.4%) for QC high. After successful validation the applicability of the method could be proven [11]. …”

Section: Liquid Chromatographymentioning

confidence: 99%

The Cortisol Steroid Levels as a Determinant of Health Status in Animals

Isaac¹,

Ibrahim²,

Andrew³

et al. 2017

J Proteomics Bioinform

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The veterinarian is daily faced with the challenge of diagnosing several disease conditions in different animals. Quantifying cortisol levels has been used in the evaluation of anxiety and distress initiated by infectious and noninfectious disease conditions, mismanagement, transportation, adverse environmental temperature and surgical operations. The hormone-cortisol has been implicated in several immunologic and metabolic processes and can thus serve as a marker to monitor animal welfare. Several factors affect the specific test to be carried out and which sample is to be used. Recently, RIA has been identified as the gold standard for determination of cortisol levels. However it also has its own short comings hence the need to utilize other assay techniques such as ELISA, fluorescence techniques and chemical assays which are more common in our environment. There is also prospect for the analysis of cortisol using non-invasive samples such finger nails as well as ear wax for enhanced test results.

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Development of an LC–MS/MS method for the determination of endogenous cortisol in hair using 13 C 3 -labeled cortisol as surrogate analyte

Cited by 48 publications

References 29 publications

Hair cortisol concentration and adrenal gland weight in healthy and ill cows

Hair cortisol concentration and adrenal gland weight in healthy and ill cows

Steroid profiling in nails using liquid chromatography-tandem mass spectrometry

The Cortisol Steroid Levels as a Determinant of Health Status in Animals

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