Development of an ISSR‐derived PCR marker linked to nematode resistance (Ha2) in spring barley

Abstract

Cereal cyst nematodes (Heterodera avenae Woll.) are economically damaging barley parasites in most cereal growing areas of the world and the development of resistant cultivars is the best measure against the pathogen. An ISSR (inter-simple sequence repeat) marker identified as closely linked with the H. avenae race 1 and 2 resistance gene (Ha2) has been converted into a codominant sequence characterized amplified region marker (Ha2S18) and mapped in barley ÔSW BuddyÕ · ÔSW CeciliaÕ DH population at 4.3 cM from the Ha2 locus on the long arm of chromosome 2H. The potential usefulness of Ha2S18 in large scale marker assisted selection schemes has been evaluated in a broad genetic background and is an important complement to the bioassay and to other linked DNA-markers for this trait.

Genetic Diversity and Variation Among Botanical Varieties of Old Portuguese Wheat Cultivars Revealed by ISSR Assays

“…In this research, we used the dominantly expressed multilocus DNA markers, inter-simple sequence repeats (ISSRs; Zietkiewicz et al 1994), which have been successfully used for cereals to assess genetic variation (Qian et al 2001;Fernández et al 2002;Qiu et al 2006), for cultivar and genotype identification (Kantety et al 1995;Nagaoka and Ogihara 1997;Pejic et al 1998;Blair et al 1999;Joshi et al 2000;Matos et al 2001), for fingerprinting of interspecific hybrids (Carvalho et al 2005), for gene tagging of interesting agronomic traits (Ammiraju et al 2001(Ammiraju et al , 2002, and for marker-assisted selection (Dayteg et al 2008).…”

Fine mapping of Rha2 in barley reveals candidate genes for resistance against cereal cyst nematode

“…Sequences associated with five RFLP markers that Kretschmer et al ( 1997 ) had mapped near the resistance locus were anchored to the pseudomolecule sequence for chromosome 2H at positions between 654,782 (mwg865) and 684,123 kbp (mwg694) (Table S4 in Online Resource 1). The sequence for which Dayteg et al ( 2008 ) had developed a SCAR marker (Ha2S18) was anchored just distal to these positions, at 685,898 kbp.…”

“…Clone sequences for five RFLP markers (mwg892, mwg865, psr901, awbma21 and mwg694) that had been mapped by Kretschmer et al ( 1997 ) and the sequence for which Dayteg et al ( 2008 ) had developed the SCAR marker Ha2S18 were subjected to a BLASTn analysis (Altschul et al 1990) against the International Barley Genome Sequencing Consortium 2H pseudomolecule (150831_barley_pseudomolecules_chr2H.fasta, downloaded from http://webblast.ipk-gatersleben.de/barley_ibsc/downloads/ ) (Mascher et al 2017 ).…”

“…While some of these markers were used for selection, they were not entirely diagnostic of resistance. To overcome some of the limitations of RFLP and SSR markers for use in marker-assisted selection, Dayteg et al ( 2008 ) developed a co-dominant sequence characterised amplified region (SCAR) marker, Ha2S18. Using a population derived from a cross between the cultivars SW Buddy and SW Cecilia, they mapped this marker on chromosome 2H and reported it to be 4.3 cM distal to Ha2 .…”