Use of Molecular Markers for Doubled Haploid Technology: From Academia to Plant Breeding Companies

Tuvesson, Stine; Larsson, Clas-Tomas; Ordon, Frank

doi:10.1007/978-1-0716-1335-1_3

Cited by 11 publications

(2 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular markers have long been deployed, in combination with flow cytometry, to determine homozygosity and identify haploids [21]. However, this method presents certain challenges due to the variability of female germplasms in the development of unique and universal InDel markers.…”

Section: Discussionmentioning

confidence: 99%

Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

et al. 2023

View full text Add to dashboard Cite

Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines for breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific haploid induction in cereals. Although an in vivo haploid induction system based on OsMTL-edited plants has been established in rice (Oryza sativa), DH technology is still limited by other factors, such as haploid identification, which is one of the essential steps required for DH technology. In the study, we addressed this technical challenge by integrating specific molecular and phenotypic markers into rice haploid inducers. We first generated large fragment insertion or deletion mutations within the OsMTL gene and designed a pair of primers flanking the mutational sites to be used as the specific and universal molecular markers between wild-type and Osmtl plants. Next, we screened for hairy leaf as a single dominant trait and integrated it into specific molecular marker-based haploid inducers using the cross and self-cross method. When crossing cytoplasmic male sterile lines with these haploid inducers, we utilized the specific InDel marker and hairy leaf phenotypic marker to identify putative haploids (or double haploids). These putative haploids were further confirmed through ploidy and phenotypic analysis, demonstrating the high efficiency of haploid identification using these markers. The haploid induction rate (HIR) of the developed specific molecular and phenotypic marker-based haploid inducers ranged from 3.7% to 12.5%. We have achieved successful integration of distinct molecular and phenotypic markers into rice haploid inducers. Our advanced marker-based system has significantly enhanced the accuracy of haploid identification, thereby expediting the adoption of DH technology in rice breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Furthermore, molecular markers based on DNA are stable, detectable in all tissues and independent of environmental or seasonal conditions. They have been employed for multiple applications in plant science such as doubled haploid (DH) technology to the identification/assessment of ‘purity’/hybrid testing, genetic diversity analysis, genetic linkage map construction, mapping of quantitative trait loci (QTLs), map-based cloning of genes, mapping of mutations, marker-assisted selection (MAS), marker assisted backcross breeding (MAB), marker-assisted pyramiding, mapping major genes, and characterization of transformants [ 56 , 57 , 58 , 59 ].…”

Section: Pistachio Taxonomy and Phylogenymentioning

confidence: 99%

History, Phylogeny, Biodiversity, and New Computer-Based Tools for Efficient Micropropagation and Conservation of Pistachio (Pistacia spp.) Germplasm

Nezami-Alanagh

Gallego

2023

Plants

View full text Add to dashboard Cite

The word “pstk” [pistag], used in the ancient Persian language, is the linguistic root from which the current name “pistachio”, used worldwide, derives. The word pistachio is generally used to designate the plants and fruits of a single species: Pistacia vera L. Both the plant and its fruits have been used by mankind for thousands of years, specifically the consumption of its fruits by Neanderthals has been dated to about 300,000 years ago. Native to southern Central Asia (including northern Afghanistan and northeastern Iran), its domestication and cultivation occurred about 3000 years ago in this region, spreading to the rest of the Mediterranean basin during the Middle Ages and finally being exported to America and Australia at the end of the 19th century. The edible pistachio is an excellent source of unsaturated fatty acids, carbohydrates, proteins, dietary fiber, vitamins, minerals and bioactive phenolic compounds that help promote human health through their antioxidant capacity and biological activities. The distribution and genetic diversity of wild and domesticated pistachios have been declining due to increasing population pressure and climatic changes, which have destroyed natural pistachio habitats, and the monoculture of selected cultivars. As a result, the current world pistachio industry relies mainly on a very small number of commercial cultivars and rootstocks. In this review we discuss and summarize the current status of: etymology, origin, domestication, taxonomy and phylogeny by molecular analysis (RAPID, RFLP, AFLP, SSR, ISSR, IRAP, eSSR), main characteristics and world production, germplasm biodiversity, main cultivars and rootstocks, current conservation strategies of both conventional propagation (seeds, cutting, and grafting), and non-conventional propagation methods (cryopreservation, slow growth storage, synthetic seed techniques and micropropagation) and the application of computational tools (Design of Experiments (DoE) and Machine Learning: Artificial Neural Networks, Fuzzy logic and Genetic Algorithms) to design efficient micropropagation protocols for the genus Pistacia.

show abstract

Haploid System in Mutation Breeding

Amirian,

Shariatpanahi,

Taghiabadi

2024

Sustainable Landscape Planning and Natural Resources Management

View full text Add to dashboard Cite

Use of Molecular Markers for Doubled Haploid Technology: From Academia to Plant Breeding Companies

Cited by 11 publications

References 77 publications

Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice

History, Phylogeny, Biodiversity, and New Computer-Based Tools for Efficient Micropropagation and Conservation of Pistachio (Pistacia spp.) Germplasm

Haploid System in Mutation Breeding

Contact Info

Product

Resources

About