Development of an indirect competitive enzyme-linked immunosorbent assay for screening ethopabate residue in chicken muscle and liver

Abstract

Development of an indirect competitive enzyme-linked immunosorbent assay for screening ethopabate residue in chicken muscle and liver.

Development of a New Monoclonal Antibody against Brevetoxins in Oyster Samples Based on the Indirect Competitive Enzyme-Linked Immunosorbent Assay

“…Hapten design is key to generating excellent antibody performances against small molecules [25]. Generally, haptens should mimic the target molecule in terms of size, shape, electronic properties, and insert functional groups such as, carboxyl, amino, and sulfhydryl groups for carrier protein coupling [16,17]. In principle, there are two ways to synthesize PbTx-2 haptens.…”

“…In principle, aldehyde groups from PbTx-2 may be conjugated with a spacer arm, such as carboxymethoxylamine hemihydrochloride (CMO) and/or aminobenzoic acid. In addition, molecular modeling has become a powerful tool in guiding and improving hapten design strategies [15][16][17][18][19]. Therefore, we explored and developed novel haptens using molecular modeling to prepare mAbs against brevetoxins.…”

An innovative hapten and monoclonal antibody-based immunoassay for determining tebuconazole residues in aqueous samples

“…Molecular model was carried out using the Gaussian 09W program and Gaussian view 5.0 (Li et al, 2017). And geometry conformations and electronic distributions for tebuconazole, hapten 1, hapten 2, hapten 3, and hapten 4 (structures see Figure 1) were evaluated in our studies.…”

“…The ELISA approach was performed referring to our previous research (Li et al, 2017). Briefly, the 96-well plates were coated with 100 μL per well of coating antigen H1-OVA diluted with carbonate-bicarbonate buffer (0.02 M CB, pH 9.6, Na 2 CO 3 1.59 g, NaHCO 3 2.93 g, diluted with 1 L water) and reacted at 37°C for 2 h. The microplates were washed 3 times with 220 μL of PBST (0.01 M, pH 7.2 PBS containing 0.1% Tween20) per well and blocked with 200 μL of CB buffer containing 2% gelatin at 37°C for 2 h. Subsequently, the samples or standard solutions (50 μL/well) and 50 μL of diluted antibodies were successively added into the plates and cultivated at 37°C for 30 min.…”

Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples

“…Immunoassays have the advantages of rapid, simple, sensitive, high throughput, economical and friendly to environment. [11][12][13][14][15][16] As a frequently-used immunoassay, enzymelinked immunosorbent assay (ELISA) has been proved to be a rapid and sensitive method for quantitative and qualitative detection of compounds. [17][18][19] The chemiluminescent enzyme immunoassay (CLEIA) also has gained attention in the research of clinical diagnosis and analytical test because of its higher sensitivity and wider dynamic range of linearity compared with colorimetric detection.…”