An innovative hapten and monoclonal antibody-based immunoassay for determining tebuconazole residues in aqueous samples

Abstract

Tebuconazole is widely applied for controlling pathogenic fungi of crops and proved hazardous to human being. To better measure tebuconazole residues, an enzyme-linked immunosorbent assay for screening tebuconazole residues in aqueous samples was established using an innovative hapten (hapten1) designed via molecular model. The work concentration of calibration standard curves based on aqueous matrices ranged from 0.51 to 10.71 ng mL-1. The cross-reactivities of other analogs were less than 6%. The detection limits of the assay were in the range of 0.197-0.297 ng mL-1 for tebuconazole in water samples. The average recoveries of tebuconazole in the spiked water samples varied from 76.76% to 107.52% with the variation coefficient scope of 4.54-13.26%. The obtained immunoassay was verified by gas chromatography coupled to mass spectrometry with high correlation coefficients (r > 0.9868). Herein, the proposed immunoassay offers a cost-effective, convenient, reliable tool for the determination of tebuconazole residues in aqueous samples.

Highly Specific Monoclonal Antibody and Sensitive Quantum Dot Beads-Based Fluorescence Immunochromatographic Test Strip for Tebuconazole Assay in Agricultural Products

“…The incubation curve of absorbance value against TEB concentration by indirect competitive ELISA shows a linear range of detection (IC 20 –IC 80 ) of 0.07–0.54 ng mL –1 and a half-maximal inhibitory concentration (IC 50 ) of 0.19 ng mL –1 (Figure A), which is 3 orders of magnitude more sensitive than reported in the previous study due in part to a longer length of the spacer arm and more appropriate active combining groups of hapten to favor the epitope exposure. − To the best of our knowledge, so far, our developed mAb shows the most sensitive antibodies for TEB detection among the reported methods. , The specificity test using 5 triazole analogues of TEB has a cross-reactivity (CR) value <0.01% (Table ).…”

“…17−19 To the best of our knowledge, so far, our developed mAb shows the most sensitive antibodies for TEB detection among the reported methods. 19,20 The specificity test using 5 triazole analogues of TEB has a crossreactivity (CR) value <0.01% (Table 1).…”

“…They developed an enzyme-linked immunosorbent assay (ELISA) for TEB with a linear detection range of 0.02–20 μg mL –1 and showed negligible or no cross-reactivity to seven other triazole compounds. However, surprisingly, we find there are only a few studies on the immunoassay for TEB, , not only the limited antibody types but also the immunoassay methods development. On this basis, in this work a high-affinity monoclonal antibody (mAb) was generated against haptens by derivatizing at the p -chloro position of the TEB molecule with a −C 5 H 10 COOH spacer arm (Figure ).…”

Rapid Detection of Tebuconazole Based on Aptasensor and Aggregation of Silver Nanoparticles

“…EU 750/2010 and 50/2016/TT-BYT) [33,34]. Moreover, in comparison with other assays for detection of tebuconazole that require expensive reagents, costly instrumentation, sample pretreatment with the toxic organic reagents such as immunoassay [35,36], liquid chromatography (LC) with UV detection (LC-UV) [37], and capillary gas chromatography with nitrogen phosphorus detection (GC-NPD) [38], this sensing platform shows its advantages in terms of simplicity, experimental cost, time for analysis, and ability for on-site testing.…”

Development of an immunochromatographic strip test for the rapid detection of soybean Bowman-Birk inhibitor

“…were determined through indirect competitive ELISA. The crossreactivities (CR) of the mAb were calculated using the following formula (Chen et al, 2017a(Chen et al, , 2019: CR = (IC 50 for BBI)/(IC 50 for competitors).…”