Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis

Wang, Xiao Lei; Wang, Yan; Ma, Limei; Zhang, Ran; De, Yanyan; Yang, Xiaowen; Wu, Qingmin

doi:10.1186/s12917-015-0436-3

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…Several types of ELISAs are there, viz ., competitive and sandwich ELISAs, which could be useful for follow-up of cases of brucellosis (Ariza et al 1992 ). Wang et al ( 2015 ) developed a highly advanced version of a monoclonal antibody-based cELISA against LPS for the diagnosis of bovine brucellosis, which revealed higher specificity than the commercially available cELISAs and RBPT (Ahmed et al 2011 ; Kirit et al 2017 ). The analysis of the anti- Brucella antibody titers of naturally infected and vaccinated cattle by indirect ELISA, SAT, indirect hemagglutination assay and microtiter plate agglutination test revealed that the naturally infected animals presented much higher titers of agglutinating antibodies in comparison to the healthy vaccinated cattle (Mohan et al 2016 ).…”

Section: Diagnosismentioning

confidence: 99%

Bovine brucellosis – a comprehensive review

Khurana

Sehrawat

Tiwari

et al. 2021

Veterinary Quarterly

139

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Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.

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Section: Diagnosismentioning

confidence: 99%

Bovine brucellosis – a comprehensive review

Khurana

Sehrawat

Tiwari

et al. 2021

Veterinary Quarterly

139

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“…The OIE recommends the use of Competitive Enzyme Linked Immunosorbent Assay (cELISA) for international cattle trade as this assay is reported to be more specific than RBPT (OIE, 2016;Rahman et al, 2019;Wang et al, 2015).…”

Section: Brucellosis Is Endemic In Both Humans and Animals Inmentioning

confidence: 99%

“…; however, the LPS antigen is also present in a number of other gram negative bacteria, including Vibrio and Yersinia enterocolitica , which may cross‐react on the brucellosis diagnostic assays (Munoz et al., 2005). The OIE recommends the use of Competitive Enzyme Linked Immunosorbent Assay (cELISA) for international cattle trade as this assay is reported to be more specific than RBPT (OIE, 2016; Rahman et al., 2019; Wang et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and risk factors for bovine brucellosis in the Chittagong Metropolitan Area of Bangladesh

Islam

Barua

Moni

et al. 2020

Veterinary Medicine & Sci

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Brucellosis is a neglected endemic zoonosis in Bangladesh and has a significant impact on public health and animal welfare of dairy farming as well as dairy farm economics. A cross‐sectional study was conducted to evaluate the seroprevalence of and risk factors for brucellosis in dairy cattle in the Chittagong metropolitan area (CMA) of Chittagong, Bangladesh. We collected serum samples (n = 158) from six randomly selected dairy farms in the CMA between February and November, 2015. The Rose Bengal Plate Test (RBPT) and a competitive ELISA (cELISA) were used as the screening and confirmatory tests respectively. Farm level and animal level demographic and risk factor data were collected using a questionnaire. The risk factors were analysed using a multivariable logistic regression with random effects. The overall seroprevalences of antibodies against brucellosis in cattle were 21.5% (34/158) and 7.6% (12/158) based on parallel and serial interpretation of the two tests respectively. Our results revealed that 20.3% (32/158) samples were positive using the RBPT and 8.9% (14/158) were positive using the cELISA. The within‐herd seroprevalence ranged from 10% to 26.3% and 5 to 20.7% using the RBPT and cELISA tests respectively. The odds of seropositivity were significantly higher in lactating cows (OR: 2.59; 95% CI: 1.02–6.55), cows producing less than 2 litres of milk (OR: 29.6; 95% CI: 4.3–353.8), cow producing 2–12 litres of milk (OR: 4.8; 95% CI: 1.1–33.4) and cows with reproductive disorders (OR: 3.2; 95% CI: 1.2–10.1). About 7.6% (12/158) and 1.3% (2/158) of cattle were found to be infected with acute and chronic brucellosis respectively. Based on these results, we suggest that cows that have reproductive disorders and are producing little milk should be prioritized for brucellosis screening in CMA. The screening tests should be used to control brucellosis in cattle in order to protect animal welfare, human health and to minimize the economic losses.

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“…Para el análisis cuantitativo de estradiol se utilizó el método inmunoenzimático de tipo competitivo ELISA (Enzyme-Linked ImmunoSorbent Assay), ya que se trata de un método no invasivo, rápido y de gran sensibilidad ( Wang et al, 2015). Las muestras de saliva (3-5 ml) fueron recogidas en Salivette ® tubos de esponja (Sarstedt, Nümbrecht, GE) y congeladas a -40ºC.…”

Section: Instrumentosunclassified

Cambios en la atención, el aprendizaje y la memoria durante las fluctuaciones de estradiol en estudiantes universitarias

Gómez-León

2019

revEDU

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With this investigation one tries to analyze the influence of the fluctuations of estradiol in the learning process of university students. The hormonal levels of 17- . estradiol have measured up in saliva, across kits ELISA (Enzyme-Linked ImmunoSorbent Assay) in 64 women, aged 18-19 years. The subjects realized the tests of memory and control atencional of Luria's battery DNA when his levels of estradiol were high and when these were lower. The studied women obtained significantly top punctuations in the curve of learning and in the tests of immediate memory and attention when they were during the more high beak of estradiol, that when the levels of estradiol were low.RESUMENCon esta investigación se pretende analizar la influencia de las fluctuaciones de estradiol en el proceso de aprendizaje de estudiantes universitarias. Se han medido los niveles hormonales de 17- β estradiol en saliva, a través de kits ELISA (Enzyme-Linked ImmunoSorbent Assay) en 64 mujeres, de entre 18 y 19 años. Los sujetos realizaron las pruebas de memoria y control atencional de la batería de Luria DNA cuando sus niveles de estradiol eran más altos y cuando estos eran más bajos. Las mujeres estudiadas obtuvieron puntuaciones significativamente superiores en la curva de aprendizaje y en las pruebas de memoria inmediata y atención cuando se encontraban durante el pico más alto de estradiol, que cuando los niveles de estradiol eran bajos.

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Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis

Cited by 13 publications

References 26 publications

Bovine brucellosis – a comprehensive review

Bovine brucellosis – a comprehensive review

Seroprevalence and risk factors for bovine brucellosis in the Chittagong Metropolitan Area of Bangladesh

Cambios en la atención, el aprendizaje y la memoria durante las fluctuaciones de estradiol en estudiantes universitarias

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