Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC--UV

Rodrigues, Ana Sofia; Lopes, Ana Rita; Leão, Ana Luiza Favarão; Couceiro, Alda; Sarmento‐Ribeiro, Ana Bela; Ramos, Fernando; Silveira, Maria Irene Noronha da; Oliveira, Catarina

doi:10.1093/chromsci/47.5.387

Cited by 21 publications

(12 citation statements)

References 10 publications

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“…There are evidence of use of adjunct antibiotics like fluoroquinolones with anticancer drugs enhancing the cytotoxic effects while, at the same time, decreasing chemotherapy-induced proinflammatory cytokine secretion from cells, which may be harmful during chemotherapeutic treatment 13,14,15 . Few spectrophotometric methods [16][17][18][19][20][21] and High-Performance Liquid Chromatography (HPLC) [22][23][24][25][26][27][28][29] have been reported for the determination of MOX and VIN in single or combined pharmaceutical dosage forms or biological fluids. But none of these methods demonstrate the simultaneous estimation of these two drugs in combination by a derivative method in the pharmaceutical dosage form.…”

Section: Fig 2: Structure Of Moxifloxacin Hydro-chloridementioning

confidence: 99%

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2019

IJPSR

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Objective: To develop a simple, accurate and precise method for the simultaneous estimation of Moxifloxacin hydrochloride (MOX) and Vinblastine sulfate (VIN). Methods: The normal spectrum of VIN and MOX were converted to its second derivative spectrum and the amplitude minima of VIN and MOX were measured at 214 nm and 297 nm, respectively. MOX and VIN solution were simultaneously determined in 0.1M HCl at 297 nm and 214 nm. Results: The amplitude of MOX and VIN were found to be more distinct in 0.1M HCl with compared to water, methanol and 0.1M NaOH (order = 2 and Δλ=1). Linearity was obtained over the range 1-8 μg/ml and 3-24 μg/ml with a lower limit of quantitation of 1.7 μg/ml and 0.4 μg/ml for MOX and VIN, respectively. For each level of samples, interand intra-day precision (% RSD) was <2.1% and <2.3%for MOX and < 2.3 and <2.7 % for VIN, respectively. The mean recovery of MOX and VIN were in the range 99.98%-103.1%and 101.03%-104.3%, respectively. The developed method was validated as per ICH guidelines for parameters like linearity, accuracy, method precision, and ruggedness. Conclusion: The results obtained were well within the acceptable criteria. The method can be used for routine analysis of MOX and VIN.

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Section: Fig 2: Structure Of Moxifloxacin Hydro-chloridementioning

confidence: 99%

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2019

IJPSR

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“…For most LC methods, reversed phase chromatographic supports were used. LC-UV methods were applied for the determination of anthracyclines in solution 67,[313][314][315][316][317]74 or in biological matrices. 67,318,319 Given the high cardiotoxicity of anthracyclines (due to the accumulation of the drug in myocardium), drug monitoring of patients is generally required.…”

Section: Intercalating Agentsmentioning

confidence: 99%

“…The analysis of vinca alkaloids has been carried out on plant extracts, [398][399][400][401][402][403] pharmaceutical formulations, 316,404 biological samples 358,359,[405][406][407][408][409][410][411][412][413][414][415][416][417][418][419][420]421 and environmental samples. 18,19,134,307,422 Vinca alcaloid extraction from plants was generally performed by ultrasound in acid media followed by LLE.…”

Section: Antitubulin Agentsmentioning

confidence: 99%

Antineoplastic drugs and their analysis: a state of the art review

Guichard

Guillarme

Bonnabry

et al. 2017

Analyst

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The number of patients suffering from cancer is constantly increasing and, consequently, the number of different chemotherapy treatments administered is increasing. Given the high reactivity and toxicity of antineoplastic drugs, analytical methods are required in all pharmaceutical fields, from drug development to their elimination in wastewater; including formulation quality control, environment and human exposure and therapeutic drug monitoring. The aim of this paper is to provide an overview of the analytical methods available for the determination of antineoplastic drugs in different matrices such as pharmaceutical formulations, biological and environmental samples. The applicability and performance of the reported methods will be critically discussed, with focus on the most commonly used antineoplastic drugs. Only conventional compounds and small molecules for targeted therapy will be considered in the present review.

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“…MTX was analyzed by HPLC with UV or fluorescence detection (Merás et al, 2002;Kuo et al, 2003), LC-MS/MS (Turci et al, 2002;Koufopantelis et al, 2009) and capillary electrophoreses (CE)-UV (Mrestani and Neubert, 1998;Flores et al, 2003Flores et al, , 2005Kuo et al, 2003Kuo et al, , 2006. VBL was analyzed by CE , LC-MS/MS (Gupta et al, 2005;Kosjek et al, 2013) and LC-UV (Tsai, 2001;Rodrigues et al, 2009). DTIC was analyzed by LC-UV (Benvenuto et al, 1981;Beitz et al, 1999;Safgren et al, 2001;Liu et al, 2008), LC-MS/MS (Liu et al, 2008), CE (Haque and Stewart, 1999) and voltammetry (Radi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…DTIC was analyzed by LC-UV (Benvenuto et al, 1981;Beitz et al, 1999;Safgren et al, 2001;Liu et al, 2008), LC-MS/MS (Liu et al, 2008), CE (Haque and Stewart, 1999) and voltammetry (Radi et al, 2014). VBL was analyzed by CE , LC-MS/MS (Gupta et al, 2005;Kosjek et al, 2013) and LC-UV (Tsai, 2001;Rodrigues et al, 2009). All published methods so far have suffered from the consumption of organic solvents, the complex pretreatment of samples or low sensitivity and selectivity when apploed in human fluids.…”

Section: Introductionmentioning

confidence: 99%

Eco‐friendly ionic liquid assisted capillary electrophoresis and α‐acid glycoprotein‐assisted liquid chromatography for simultaneous determination of anticancer drugs in human fluids

El‐Hady

Albishri

Rengarajan

2014

Biomedical Chromatography

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In the current work, two eco-friendly analytical methods based on capillary electrophoresis (CE) and reversed phase liquid chromatography (RPLC) were developed for simultaneous determination of the most commonly used anticancer drugs for Hodgkin's disease: methotrexate (MTX), vinblastine, chlorambucil and dacarbazine. A background electrolyte (BGE) of 12.5 mmol/L phosphate buffer at pH 7.4 and 0.1 µmol/L 1-butyl-3-methyl imidazolium bromide (BMImBr) ionic liquid (IL) was used for CE measurements at 250 nm detection wavelength, 20 kV applied voltage and 25 °C. The rinsing protocol was significantly improved to reduce the adsorption of IL on the interior surface of capillary. Moreover, RPLC method was developed on α-1-acid glycoprotein (AGP) column. Mobile phase was 10 mmol/L phosphate buffer at pH 6.0 (100% v/v) and flow rate at 0.1 mL/min. As AGP is a chiral column, it was successfully separated l-MTX from its enantiomer impurity d-MTX. Good linearity of quantitative analysis was achieved with coefficients of determinations (r(2) ) >0.995. The stability of drugs measurements was investigated with adequate recoveries up to 24 h storage time under ambient temperature. The limits of detection were <50 and 90 ng/mL by CE and RPLC, respectively. The using of short-chain IL as an additive in BGE achieved 600-fold sensitivity enhancement compared with conventional Capillary Zone Electrophoresis (CZE). Therefore, for the first time, the proposed methods were successfully applied to determine simultaneously the analytes in human plasma and urine samples at clinically relevant concentrations with fast and simple pretreatments. Developed IL-assisted CE and RPLC methods were also applied to measure MTX levels in patients' samples over time.

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Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC--UV

Cited by 21 publications

References 10 publications

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Antineoplastic drugs and their analysis: a state of the art review

Eco‐friendly ionic liquid assisted capillary electrophoresis and α‐acid glycoprotein‐assisted liquid chromatography for simultaneous determination of anticancer drugs in human fluids

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