Development of a three-layer consecutive gene delivery system for enhanced bone regeneration

Kim, Hye Jin; Park, Jong Min; Cho, Hui Bang; Park, Ji-In; Park, Ji Sun; Park, Keun-Hong

doi:10.1016/j.biomaterials.2021.121104

Cited by 12 publications

(8 citation statements)

References 29 publications

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“…It is challenging to meet the whole repair process with a bare scaffold, as structures must emulate the native microenvironment of bone cells at the same time augment their proliferation and other differentiation processes. Adding active substances, such as drugs, [67][68][69] short peptides, [70][71][72] growth factors, [73,74] and some cells or genes [75,76] can better promote bone repair and regeneration. The reason is that the nutrients required for each stage differ because the early stages of healing focus on anti-inflammatory, angiogenesis, and cell recruitment, [77] while the later ones focus more on osteogenic differentiation.…”

Section: Scaffolds For Bone Regenerationmentioning

confidence: 99%

Advanced strategies of scaffolds design for bone regeneration

Song,

Li,

Fang

et al. 2023

BMEMat

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Bone defects are encountered substantially in clinical practice, and bionic scaffolds represent a promising solution for repairing bone defects. However, it is difficult to fabricate scaffolds with bionic structures and reconstruct the microenvironment to fulfill the satisfying repair effects. In this review article, we first discuss various strategies for the design and construction of bionic scaffolds to promote bone defect repair, especially including the structural construction of the scaffold and the integration of bioactive substances together with the application of external stimuli. We then discuss the roles of artificial intelligence and medical imaging in aiding clinical treatment. Finally, we point out the challenges and future outlooks in developing multifunctional bone repair scaffolds, aiming to provide insights for improving bone regeneration efficacy and accelerating clinical translation.

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Section: Scaffolds For Bone Regenerationmentioning

confidence: 99%

Advanced strategies of scaffolds design for bone regeneration

Song,

Li,

Fang

et al. 2023

BMEMat

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“…They were loaded on 80, 50, and 20 nm gold nanoparticles to induce osteogenic differentiation of MSCs through sequential gene delivery. Osteogenic differentiation of hMSCs was confirmed, and the bone defect was repaired by transplanting hMSCs internalized with a T‐CGDS (Figure 3) (Kim et al., 2021).…”

Section: Non‐viral Delivery Of Plasmids For Bone Repairmentioning

confidence: 99%

“…In a recent study, ADSCs transduced with lentivirus containing SDF‐1 did not influence osteogenic differentiation or expression of osteogenic genes, but caused significant osteoblastic formation of endogenous osteoblasts. The Methyl‐accepting chemotaxis protein scaffolds used in this study enhanced the osteogenic differentiation of endogenous BMSCs (Kim et al., 2021).…”

Section: Viral Gene Therapy Applications For Bone Regenerationmentioning

confidence: 99%

Recent advances in gene therapy for bone tissue engineering

Ranjbarnejad

Khazaei

Shahryari³

et al. 2022

J Tissue Eng Regen Med

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Autografting, a major treatment for bone fractures, has potential risks related to the required surgery and disease transmission. Bone morphogenetic proteins (BMPs) are the most common osteogenic factors used for bone-healing applications. However, BMP delivery can have shortcomings such as a short half-life and the high cost of manufacturing the recombinant proteins. Gene delivery methods have demonstrated promising alternative strategies for producing BMPs or other osteogenic factors using engineered cells. These approaches can also enable temporal overexpression and local production of the therapeutic genes in the target tissues. This review addresses recent progress on engineered viral, non-viral, and RNA-mediated gene delivery systems that are being used for bone repair and regeneration. Advances in clustered regularly interspaced short palindromic repeats/Cas9 genome engineering for bone tissue regeneration also is discussed.

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“…Gold NPs can function as vehicles for delivering all sizes of therapeutic agents and biomolecules, such as proteins, peptides, growth factors, and DNA. Kim et al [39] developed a gene delivery system with a three-layered putamen structure using AuNPs with different diameters to promote bone regeneration and osteogenic differentiation. Functional gene fragments of activating transcription factor (ATF) 4, osterix (transcription factor SP7), and runt-related transcription factor 2 (RUNX2) combined with AuNPs improved osteogenesis efficiency via cell internalisation.…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Tissue-Engineered Nanomaterials Play Diverse Roles in Bone Injury Repair

et al. 2023

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Nanomaterials with bone-mimicking characteristics and easily internalized by the cell could create suitable microenvironments in which to regulate the therapeutic effects of bone regeneration. This review provides an overview of the current state-of-the-art research in developing and using nanomaterials for better bone injury repair. First, an overview of the hierarchical architecture from the macroscale to the nanoscale of natural bone is presented, as these bone tissue microstructures and compositions are the basis for constructing bone substitutes. Next, urgent clinical issues associated with bone injury that require resolution and the potential of nanomaterials to overcome them are discussed. Finally, nanomaterials are classified as inorganic or organic based on their chemical properties. Their basic characteristics and the results of related bone engineering studies are described. This review describes theoretical and technical bases for the development of innovative methods for repairing damaged bone and should inspire therapeutic strategies with potential for clinical applications.

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Development of a three-layer consecutive gene delivery system for enhanced bone regeneration

Cited by 12 publications

References 29 publications

Advanced strategies of scaffolds design for bone regeneration

Advanced strategies of scaffolds design for bone regeneration

Recent advances in gene therapy for bone tissue engineering

Tissue-Engineered Nanomaterials Play Diverse Roles in Bone Injury Repair

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