Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage

Abstract

We have developed a novel bioassay method for the detection of snake venom based on the permeability of endothelial cell monolayers cultured in Transwell cell culture inserts. This assay relies on the proteolytic degradation of capillary basement membrane proteins, a pathophysiological event that occurs due to snakebites in vivo. Transwell permeability assays with fluorescence measurements are advantageous with regard to ethical considerations for the use of animals. The assay time was reduced from 24 h for animal tests to 2 h, and many samples could be assayed easily.

Environmental aluminum oxide inducing neurodegeneration in human neurovascular unit with immunity

“…Prior to testing potential risks of AlNPs on the BBB, we validated the formation of tight-junctions properly in the BBB side of our model by estimation of an apparent permeability coefficient of 40 kDa fluorescein isothiocyanate (FITC)–dextran (Pe = 8.5 × 10 −7 cm/s) 15 , 18 , 19 . To explore the influence of AlNPs on the BBB, we added the FITC-dextran along with or without AlNPs to the L.C.…”

Recapitulating Solid Stress on Tumor on a Chip for Nanomedicine Diffusive Transport Prediction

“…ToCs have been used to get a better understanding of cancer progression and metastasis, [16][17][18][19][20] as well as angiogenesis and blood vessel formation. [21][22][23][24] ToC devices have been also developed for the evaluation of new therapeutic approaches against cancer, including nanomedicines. [25][26][27][28] However, up-to-date, ToC devices have failed to model some of the main critical components involved in nanomedicine delivery to the tumor site.…”