Air-pressure-driven Separable Microdevice to Control the Anisotropic Curvature of Cell Culture Surface

Yamashita, Tadahiro; Nishina, Takuya; Matsushita, Ichiro; Sudo, Ryo

doi:10.2116/analsci.20a001

Cited by 4 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, as demonstrated by recent studies, cells are able to respond to curvatures up to 1000 μm, a phenomenon defined as "curvotaxis," which may result in cell re-orientation and different gene expression. [159][160][161][162] On typical cell culture systems subject to outof-plane deformation the effective radius of curvature of the membrane is out of the cell "curvature" sensing range and, consequently, they are likely to "feel" an in-plane deformation. Reproducing the in vivo curvature is important to mimic the cell native environment and the out-ofplane deformations at the cell scale.…”

Section: A Brief Description Of the Motionmentioning

confidence: 99%

Breathing in vitro: Designs and applications of engineered lung models

et al. 2021

View full text Add to dashboard Cite

The aim of this review is to provide a systematic design guideline to users, particularly engineers interested in developing and deploying lung models, and biologists seeking to identify a suitable platform for conducting in vitro experiments involving pulmonary cells or tissues. We first discuss the state of the art on lung in vitro models, describing the most simplistic and traditional ones. Then, we analyze in further detail the more complex dynamic engineered systems that either provide mechanical cues, or allow for more predictive exposure studies, or in some cases even both. This is followed by a dedicated section on microchips of the lung. Lastly, we present a critical discussion of the different characteristics of each type of system and the criteria which may help researchers select the most appropriate technology according to their specific requirements. Readers are encouraged to refer to the tables accompanying the different sections where comprehensive and quantitative information on the operating parameters and performance of the different systems reported in the literature is provided.

show abstract

Section: A Brief Description Of the Motionmentioning

confidence: 99%

Breathing in vitro: Designs and applications of engineered lung models

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Biomaterial design based on mechanobiology for the development of cell culture and analysis has attracted attention in the field of cell biology and in medical applications. [1][2][3] In the field of regenerative medicine, the elastic modulus of cell culture substrates has been noticed as a cue to reliably and precisely control lineage commitment and the fate of stem cells. [4][5][6][7] Mesenchymal stem cell (MSC) is a type of stem cell that is highly mechanosensitive.…”

Section: Introductionmentioning

confidence: 99%

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

et al. 2021

View full text Add to dashboard Cite

To establish a guideline for the design of cell culture substrates to control human mesenchymal stem cell (MSC) differentiation, we quantitatively characterized the heterogeneity in the responsiveness of MSCs to the elastic modulus of culture substrates. We analyzed the elastic modulus-dependent dynamics of a mechanotransducer, YAP, and an osteogenic differentiation factor, RUNX2, in three different MSC lots using a styrenated gelatin gel with controllable elastic modulus. The percentage of cells with YAP in the nucleus increased linearly with increases in the elastic modulus, reaching a plateau at 10 kPa for all the lots analyzed. The increase in the percentage with the substrate elastic modulus was described by the same linear function. The percentage of cells with RUNX2 nuclear localization also increased linearly with increases in the substrate elastic modulus, plateauing at 5 kPa, although the regression lines to the linearly increasing regions varied between lots. These similarities and differences in YAP and RUNX2 dynamics among cell populations are basis to design the substrate elastic modulus to manipulate YAP and RUNX2 localizations.

show abstract

Milliscale Substrate Curvature Promotes Myoblast Self‐Organization and Differentiation

Connon

Gouveia

2021

Advanced Biology

View full text Add to dashboard Cite

Biological tissues comprise complex structural environments known to influence cell behavior via multiple interdependent sensing and transduction mechanisms. Yet, and despite the predominantly nonplanar geometry of these environments, the impact of tissue‐size (milliscale) curvature on cell behavior is largely overlooked or underestimated. This study explores how concave, hemicylinder‐shaped surfaces 3–50 mm in diameter affect the migration, proliferation, orientation, and differentiation of C2C12 myoblasts. Notably, these milliscale cues significantly affect cell responses compared with planar substrates, with myoblasts grown on surfaces 7.5–15 mm in diameter showing prevalent migration and alignment parallel to the curvature axis. Moreover, surfaces within this curvature range promote myoblast differentiation and the formation of denser, more compact tissues comprising highly oriented multinucleated myotubes. Based on the similarity of effects, it is further proposed that myoblast susceptibility to substrate curvature depends on mechanotransduction signaling. This model thus supports the notion that cellular responses to substrate curvature and compliance share the same molecular pathways and that control of cell behavior can be achieved via modulation of either individual parameter or in combination. This correlation is relevant for elucidating how muscle tissue forms and heals, as well as for designing better biomaterials and more appropriate cell–surface interfaces.

show abstract

Air-pressure-driven Separable Microdevice to Control the Anisotropic Curvature of Cell Culture Surface

Cited by 4 publications

References 39 publications

Breathing in vitro: Designs and applications of engineered lung models

Breathing in vitro: Designs and applications of engineered lung models

Designing Elastic Modulus of Cell Culture Substrate to Regulate YAP and RUNX2 Localization for Controlling Differentiation of Human Mesenchymal Stem Cells

Milliscale Substrate Curvature Promotes Myoblast Self‐Organization and Differentiation

Contact Info

Product

Resources

About