Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique

Prakash, B.S.; Meyer, H. H. D.; Schallenberger, E.; Wiel, D.F.M. van de

doi:10.1016/0022-4731(87)90389-x

Cited by 263 publications

(131 citation statements)

References 14 publications

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“…toleration, sweating, and vaginal mucus) to determine standing heat, which occurred around 60 h after Estrumate injection. Blood samples were taken at day of synchronization, day 0 of the estrous cycle and just before slaughtering to determine serum P 4 (Prakash et al 1987) and estradiol-17b (E 2 ; Meyer et al 1990) levels. The pregnant groups of animals were inseminated with cryo-conserved sperm at estrus (AI) and slaughtered at days 12, 15, and 18 of gestation respectively (nZ5 per group).…”

Section: Pretreatment Of Animals and Collection Of Endometrial And Trmentioning

confidence: 99%

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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Prostaglandins (PGs) are important regulators of reproductive processes including early embryonic development. We analyzed the most relevant PG in bovine uteri at different preimplantation pregnancy stages when compared with non-pregnant controls. Additionally, endometrium and trophoblast tissues were examined regarding specific enzymes and receptors involved in PG generation and function. Simmental heifers were artificially inseminated or received seminal plasma only. At days 12, 15, or 18, post-estrus uteri were flushed for PG determination by liquid chromatography-tandem mass spectrometry. Endometrium and trophoblast tissues were sampled for RNA extraction and quantitative real-time PCR analysis. At all days and points of time examined, the concentration of 6-keto PGF 1a (stable metabolite of PGI 2 ) was predominant followed by PGF 2a OPGE 2 OPGD 2 zTXB 2 (stable metabolite of TXA 2 ). At days 15 and 18, PG increased from overall low levels at day 12, with a much more pronounced increase during pregnancy. The PGF 2a /PGE 2 ratio was not influenced by status. The highest PG concentration was measured at day 15 with 6-keto PGF 1a (6.4 ng/ml) followed by PGF 2a (1.1 ng/ml) and PGE 2 (0.3 ng/ml). Minor changes in endometrial PG biosynthesis enzymes occurred due to pregnancy. Trophoblasts revealed high transcript abundance of general and specific PG synthases contributing to uterine PG. As PGI 2 and PGF 2a receptors were abundantly expressed by the trophoblast, abundant amounts of PGI 2 and PGF 2a in the uterine lumen point towards an essential role of PG for the developing embryo. High amounts of PG other than PGE 2 in the preimplantation uterus may be essential rather than detrimental for successful reproduction.

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Section: Pretreatment Of Animals and Collection Of Endometrial And Trmentioning

confidence: 99%

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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“…The concentration of progesterone in blood plasma was measured after extraction with petrol ether using an enzyme immunoassay technique (Prakash et al 1987). Progesterone-6-hydroxy-hemisuccinate horseradish peroxidase was used as enzyme solution.…”

Section: Corpora Luteamentioning

confidence: 99%

Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum

Kliem¹,

Welter²,

Kraetzl³

et al. 2007

Reproduction

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“…The used methods were previously validated for use in culture medium by using antisera against steroids produced in the Institute of Animal Science, Neustadt (Germany). Progesterone concentrations were measured by using EIA as described previously by Prakash et al (1987). Rabbit antiserum against progesterone was obtained from Research Institute for Animal Production, Schoonoord (the Netherlands).…”

Section: Immunoassaymentioning

confidence: 99%

Can xylene and chia (<i>Salvia hispanica</i> L.) seed extract directly affect basic bovine ovarian cell functions?

et al. 2017

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Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique

Cited by 263 publications

References 14 publications

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum

Can xylene and chia (<i>Salvia hispanica</i> L.) seed extract directly affect basic bovine ovarian cell functions?

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