Can xylene and chia (&lt;i&gt;Salvia hispanica&lt;/i&gt; L.) seed extract
directly affect basic bovine ovarian cell functions?

Tarko, Adam; Štochmaľová, Aneta; Hrabovszká, Sandra; Vachanová, A; Harrath, Abdel Halim; Grossman, R.; Sirotkin, Alexander V.

doi:10.22358/jafs/69097/2017

Cited by 6 publications

(12 citation statements)

References 14 publications

(22 reference statements)

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“…Similarly, another study on patients with pancreatic cancer (Angst et al, 2013) suggests a possible benefit of quercetin. However, these results contradict the findings of Leśniak-Walentyn et al (2013), who demonstrated increased proliferation (Shu et al, 2011) and decreased apoptosis in rabbit (Shu et al, 2011;Tarko et al, 2018) and decreased apoptosis in bovine (Tarko et al, 2018) ovaries cultured with quercetin. The study (Tarko et al, 2018) also demonstrated an inhibitory effect of quercetin on progesterone release.…”

Section: Does Quercetin Affect Ovarian Cell Functions?mentioning

confidence: 61%

“…We obtained 12 ovaries from 3-to 5-month-old Landrace breed specimens at the follicular stage of the estrous cycle from the slaughterhouses of the National Agricultural Food Centre in Nitra and in Stará Myjava. The ovarian granulosa cells were prepared according to Tarko et al (2017). After 96 h of culture, when the cells created a monolayer of 75 %, the medium was replaced with a fresh one that was supplemented with one of the following treatment conditions: (1) control groups without treatment of quercetin (Q) (for research use only, sc-206089A, Santa Cruz Biotechnology) extract (AppliChem GmbH, Germany; 0 µg mL −1 of quercetin), (2) groups treated with Q extract (1, 10, and 100 µg mL −1 ), (3) a control group treated with benzene (20 and 0 µg mL −1 of Q) (Reag.…”

Section: Preparation Processing and Culture Of Ovarian Granulosa Cellsmentioning

confidence: 99%

“…Proliferation (PCNA) and apoptosis (BAX) markers were detected via immunocytochemistry using the method described by Osborn and Isenberg (1994) and Tarko et al (2017). The presence of markers of proliferation and apoptosis was detected by immunocytochemistry (Osborn and Isenberg, 1994).…”

Section: Immunocytochemistry (Icc)mentioning

confidence: 99%

“…It may do so, for example, by suppressing tumour cell proliferation (Jeong, 2008;Warren, 2009). The action of quercetin on healthy ovarian cells has been demonstrated previously on rabbit (Leśniak-Walentyn et al, 2013), mouse (Shu et al, 2011) and bovine (Tarko et al, 2018) ovaries; however, the action of quercetin on porcine reproductive processes and the ability of quercetin to protect these processes against environmental contaminants, including benzene, have not yet been studied.…”

Section: Introductionmentioning

confidence: 97%

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Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release

et al. 2019

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We hypothesized that the environmental contaminant benzene and the plant antioxidant quercetin may affect ovarian cell functions and that quercetin could offer protection against the adverse effects of benzene. This study aimed to examine the action of benzene, quercetin, and their combination on porcine ovarian granulosa cell functions. We elucidated the effects of benzene (20 unitµg mL-normal1), quercetin (at the doses 0, 1, 10, 100 unitµg mL-normal1), and their combination on ovarian granulosa cell functions (proliferation, apoptosis, and hormone release) in vitro using immunocytochemistry and enzyme immunoassay respectively. Benzene alone stimulated proliferation, apoptosis, and oxytocin release and inhibited progesterone and prostaglandin F release. Quercetin alone inhibited proliferation, apoptosis, and stimulated oxytocin release but did not affect progesterone and prostaglandin F release. When used in combination with benzene, quercetin promoted the inhibitory effect of benzene on progesterone release. Overall, these data suggest that benzene and quercetin have direct stimulatory and inhibitory effects, respectively, on basic ovarian functions. Moreover, no protective action of quercetin against the effects of benzene was found. Rather, it was found to enhance the effect of benzene on progesterone release. Therefore, quercetin cannot be considered for preventing or mitigating the effects of benzene on reproductive processes.

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Section: Does Quercetin Affect Ovarian Cell Functions?mentioning

confidence: 61%

Section: Preparation Processing and Culture Of Ovarian Granulosa Cellsmentioning

confidence: 99%

Section: Immunocytochemistry (Icc)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release

et al. 2019

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“…Therefore, it improved the ovaries' health by increasing the secretion of estradiol, FSH, and LH (Izaguirry et al, 2017; Nna, Usman, Ofutet, & Owu, 2017). Since QUR is one of the main components of hydrolyzed and crude extracts of chia seeds (Reyes‐Caudillo, Tecante, & Valdivia‐López, 2008), Tarko et al used xylene, as an environmental contaminant, and different doses of chia seed extract (1, 10 and 100 ng ml −1 ) separately or together to investigate their effects on bovine ovarian cells activity (Tarko et al, 2017). Xylene when given alone induced the proliferation of bovine ovarian cells and inhibited the release of testosterone and progesterone.…”

Section: In Vitro and In Vivo Studiesmentioning

confidence: 99%

Overview of biological effects of Quercetin on ovary

Rashidi

Khosravizadeh

Talebi

et al. 2020

Phytotherapy Research

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Over the last few decades, using natural products has been increased to treat different diseases. Today, great attention has been pointed toward the usage of natural products such as flavonoids, especially Quercetin (QUR), in the treatment of diseases. QUR as a natural antioxidant has been traditionally used to prevent or treat a variety of diseases such as cancer, cardiovascular disease, polycystic ovary syndrome (PCOS), obesity, chronic inflammation, and reproductive system dysfunction. Several studies demonstrated that QUR acts as an anti‐inflammatory, anti‐apoptotic, antioxidant, and anticancer agent. With this in view, in this study, we intended to describe an overview of the biological effects of QUR on the ovary. QUR improves the quality of oocytes and embryos. It affects the proliferation and apoptosis and decreases the oxidative stress in granulosa cells (GCs). Furthermore, QUR can be used as a complementary and alternative therapy in ovarian cancer and it has beneficial effects in the treatment of PCOS patients. It seems that QUR as a supplementary factor has different activities for the treatment of different disorders and it also has bidirectional activities. However, further investigations are needed for understanding the efficacy of QUR in the treatment and improvement of gynecological patients.

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Quercetin protects human granulosa cells against oxidative stress via thioredoxin system

Rashidi

Aleyasin

Eslami

et al. 2019

Reproductive Biology

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Can xylene and chia (<i>Salvia hispanica</i> L.) seed extract directly affect basic bovine ovarian cell functions?

Cited by 6 publications

References 14 publications

Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release

Effects of benzene, quercetin, and their combination on porcine ovarian cell proliferation, apoptosis, and hormone release

Overview of biological effects of Quercetin on ovary

Quercetin protects human granulosa cells against oxidative stress via thioredoxin system

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