Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum

Kliem, H.; Welter, Harald; Kraetzl, Wolf-Dieter; Šteffl, Martin; Meyer, Heinrich; Schams, D.; Berisha, Bajram

doi:10.1530/rep-06-0172

Cited by 55 publications

(63 citation statements)

References 61 publications

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“…Several of these proteases are upregulated by the LH surge (e.g. MMP1, MMP9, MMP13, MT-MMP1 as well tissue and urokinase plasminogen activators), while others such as MMP2 are not (Bakke et al 2002, 2004, Dow et al 2002, Kliem et al 2007, Berisha et al 2008. These proteinases are nevertheless integral components in the ovulatory process.…”

Section: Periovulatory Events: the Breakdown Of The Basement Membranementioning

confidence: 99%

Angiogenesis and vascular function in the ovary

Robinson

Woad²,

Hammond³

et al. 2009

Reproduction

239

210

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Ovarian function is dependent on the establishment and continual remodelling of a complex vascular system. This enables the follicle and/or corpus luteum (CL) to receive the required supply of nutrients, oxygen and hormonal support as well as facilitating the release of steroids. Moreover, the inhibition of angiogenesis results in the attenuation of follicular growth, disruption of ovulation and drastic effects on the development and function of the CL. It appears that the production and action of vascular endothelial growth factor A (VEGFA) is necessary at all these stages of development. However, the expression of fibroblast growth factor 2 (FGF2) in the cow is more dynamic than that of VEGFA with a dramatic upregulation during the follicular-luteal transition. This upregulation is then likely to initiate intense angiogenesis in the presence of high VEGFA levels. Recently, we have developed a novel ovarian physiological angiogenesis culture system in which highly organised and intricate endothelial cell networks are formed. This system will enable us to elucidate the complex inter-play between FGF2 and VEGFA as well as other angiogenic factors in the regulation of luteal angiogenesis. Furthermore, recent evidence indicates that pericytes might play an active role in driving angiogenesis and highlights the importance of pericyte-endothelial interactions in this process. Finally, the targeted promotion of angiogenesis may lead to the development of novel strategies to alleviate luteal inadequacy and infertility.

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Section: Periovulatory Events: the Breakdown Of The Basement Membranementioning

confidence: 99%

Angiogenesis and vascular function in the ovary

Robinson

Woad²,

Hammond³

et al. 2009

Reproduction

239

210

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“…Non-specific protein binding was eliminated by incubation in 10 % normal goat serum in phosphate buffered saline (PBS) for 1 h at 20°C. Sections were then incubated with, a polyclonal rabbit antibody against MMP14 (RB-1544), a monoclonal mouse antibody against MMP2 (MS-806, Ab-4) (all purchased from Lab Vision, Newmarket, Suffolk, UK) (Kliem et al, 2007) as well as a monoclonal mouse antibody against TIMP2 (clone 3A4, antibodies-online GmbH, Aachen, Germany). Each antibody was used at a dilution of 4.0 µg/mL.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy

Ulbrich

Meyer

Zitta

et al. 2011

Molecular and Cellular Endocrinology

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Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy. Molecular and Cellular Endocrinology, Elsevier, 2010, 332 (1-2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…The Bioanalyzer electropherogram of total RNA shows two distinct ribosomal peaks corresponding to either 18S or 28S for eukaryotic RNA, and a relatively flat baseline between the 5S and 18S ribosomal peaks. The automatically calculated RNA integrity number (RIN) allows classification of total RNA based on a numbering system from 1 to 10, with 1 being the most degraded profile and 10 being the most intact [32].…”

Section: Isolation Of Total Rnamentioning

confidence: 99%

“…In order to obtain the cycle threshold difference (DCP), the GAPDH-normalized data were analyzed by the DDCP method described previously by Livak and Schmittgen [45]. Therefore, DCP was not subtracted from a control group, but from the value 40 (arbitrary value), so that a high 40 À DCP value indicated a high gene expression level, and vice versa [32].…”

Section: Targetmentioning

confidence: 99%

“…Some of the locally produced regulators of luteal angiogenesis also seem to be very important during luteal regression, and these include members of the following growth factor systems: vascular endothelial growth factor (VEGF) [15][16][17], angiopoietin (ANGPT) [18][19][20][21], fibroblast growth factors (FGFs) [17,22,23], insulin-like growth factor (IGF) [24][25][26], nitric oxide [27,28], angiotensin and endothelin systems [5,[29][30][31], extracellular matrix degrading proteases and different cytokines, apoptotic factors, and enzymes [10,[32][33][34][35]. Most of these studies have been done at the end of functional luteolysis (about 12 h after PTGF administration) or during structural luteolysis (after 12 h).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Prostaglandin F2 Alpha on Local Luteotropic and Angiogenic Factors During Induced Functional Luteolysis in the Bovine Corpus Luteum1

Berisha

Meyer²,

Schams³

2010

Biology of Reproduction

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The essential role of endometrial prostaglandin F2 alpha (PTGF) for induction of the corpus luteum (CL) regression is well documented in the cow. However, the acute effects of PTGF on known local luteotropic factors (oxytocin [OXT] and its receptor, insulin-like growth factor [IGF] 1, and progesterone and its receptor), the principal angiogenic factor vascular endothelial growth factor (VEGF) A and the capillary destabilization factor angiopoietin (ANGPT) 2 were not thoroughly studied in detail. The aim of this study was therefore to evaluate the tissue concentration of these factors during PTGF induced luteolysis. In addition the mRNA expression of progesterone receptor (PGR), OXT receptor (OXTR), IGF1, IGFBP1, ANGPT1, and ANGPT2 was determined at different times after PTGF treatment. Cows (n = 5 per group) in the mid-luteal phase (Days 8-12, control group) were injected with the PTGF analog (cloprostenol), and CL were collected by transvaginal ovariectomy at 0.5, 2, 4, 12, 24, 48, and 64 h after injection. The mRNA expression was analyzed by quantitative real-time PCR, and the protein concentration was evaluated by enzyme immunoassay or radioimmunoassay. Progesterone concentrations, as well as mRNA expression of PGR, in CL tissue were significantly down-regulated by 12 h after PTGF. Tissue OXT peptide and OXTR mRNA decreased significantly after 2 h, followed by a continuous decrease of OXT mRNA. IGF1 and VEGFA protein already decreased after 0.5 h. By contrast, the IGFBP1 mRNA was up-regulated significantly after 2 h to a high plateau. ANGPT2 protein and mRNA significantly increased during the first 2 h, followed by a steep decrease after 4 h. The acute decrease of local luteotropic activity and acute changes of ANGPT2 and VEGFA suggest that modulation of vascular stability may be a key component in the cascade of events leading to functional luteolysis.

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Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum

Abstract: The corpus luteum (CL) offers the opportunity to study high proliferative processes during its development and degradation processes during its regression. We examined the mRNA expression of matrix metalloproteases (MMP)-1, MMP-2, MMP-9,

Cited by 55 publications

References 61 publications

Angiogenesis and vascular function in the ovary

Angiogenesis and vascular function in the ovary

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy

Effect of Prostaglandin F2 Alpha on Local Luteotropic and Angiogenic Factors During Induced Functional Luteolysis in the Bovine Corpus Luteum1

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