Development of a Reproductive Performance Test for Endocrine Disrupting Chemicals Using Pair-Breeding Fathead Minnows (<i>Pimephales promelas</i>)

Harries, Jule E.; Runnalls, Tamsin J.; Hill, Elisabeth M.; Harris, Ciaran; Maddix, Susan; Sumpter, John P.; Tyler, Charles R.

doi:10.1021/es991292a

Cited by 205 publications

(167 citation statements)

References 50 publications

(64 reference statements)

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“…This supports the findings of the previous experiments, in which the lengths, weights, and gonadosomatic indices of both the fathead minnows and three-spined sticklebacks exposed to various concentrations of phthalate ester were unaffected by exposure [24].…”

Section: Water Chemistrysupporting

confidence: 91%

Evidence suggesting that di‐n‐butyl phthalate has antiandrogenic effects in fish

Aoki

Harris

Katsiadaki

et al. 2011

Enviro Toxic and Chemistry

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Abstract-Phthalate ester plasticizers are anti-androgenic in mammals. High doses of certain phthalates consistently interfere with the normal development of male offspring exposed in utero, causing disrupted sperm production, abnormal development of the genitalia, and in some cases, infertility. In the environment, phthalates are considered ubiquitous, and are commonly measured in aquatic ecosystems at low ng to µg per litre concentrations. Given the similarity between mammalian and teleost endocrine systems, phthalate esters may be able to cause anti-androgenic endocrine disruption in fish in the wild. In the present study, adult male three-spined sticklebacks (Gasterosteus aculetaus) (n=8) were exposed to di-n-butyl phthalate (DBP) (0, 15, and 35 µg DBP/L) for 22 d and analyzed for changes in nesting behavior, plasma androgen concentrations, spiggin concentrations, and steroidogenic gene expression. Plasma testosterone concentrations were significantly higher in males from the 35 µg DBP/L group compared to the solvent 2 control, while plasma 11-ketotestosterone concentrations were not significantly affected.Expression of steroid acute regulatory protein and 3β-hydroxysteroid dehydrogenase remained unchanged. Spiggin concentrations were significantly lower in the males exposed to 35 µg DBP/L. Nest-building appeared to be slower in some males exposed to DBP, but this was not statistically significant. These results suggest that DBP has antiandrogenic effects in fish. However, further research is required to firmly establish the consequences of chronic DBP exposure in fish.

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Section: Water Chemistrysupporting

confidence: 91%

Evidence suggesting that di‐n‐butyl phthalate has antiandrogenic effects in fish

Aoki

Harris

Katsiadaki

et al. 2011

Enviro Toxic and Chemistry

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“…Scholz and Gutzeit (2000) reported GSI of male medaka was decreased at EE 2 exposure levels of 10 and 100 ng/l, respectively. Decreased GSI was also observed when male fathead minnows (Pimephales promelas) were exposed at 48 and 58.6 lg NP/l for 21 d (Harries et al, 2000). Significantly higher HSI of males could only be observed at 25 ng/l EE 2 treatments (Table 1).…”

Section: Tissue Somatic Indicesmentioning

confidence: 85%

Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol

Zha¹,

Wang²,

Wang³

et al. 2007

Chemosphere

156

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Adult rare minnow (Gobiocypris rarus) were exposed to 0, 1, 5, and 25 ng/l (nominal concentrations) of 17a-ethynylestradiol (EE 2 ) and 3, 10, and 30 lg/l (nominal concentrations) of 4-nonylphenol (NP) under flow-through conditions for a period of 28 d. Low mortality was observed at 5 and 25 ng/l EE 2 and the growth of fish reduced significantly at 25 ng/l EE 2 compared to controls. However, the gonadosomatic indices (GSI) of male fish were significantly higher in 1 ng/l EE 2 treatments and in 10 and 30 lg/l NP treatments (p < 0.05). Renal somatic indices (RSI) of male fish in EE 2 treatments were significantly higher than those in controls (p < 0.05). In contrast, significantly decreased GSI and RSI of female fish could only be observed in 5 and 25 ng/l EE 2 treatments (p < 0.05). Hepatosomatic indices (HSI) of male fish were significantly higher in 25 ng/l EE 2 treatments. However, significantly increased of HSI of female fish could only be observed in 1 ng/l EE 2 treatments. Plasma vitellogenin (VTG) induction could be observed in males after exposed to different concentrations of EE 2 and NP, and plasma VTG concentrations in females exposed to 5 and 25 ng/l EE 2 were also significantly higher than in controls (p < 0.05). At level higher than 5 ng/l EE 2 or 30 lg/l NP, hepatic tissue and renal tissue impairment of males could be observed. The pathological male liver was associated with a hypertrophy of hepatocytes and damages to cellar structure and accumulated eosinophilic material. Renal tissue showed different pathological effects which was reflected by accumulated eosinophilic material, hemorrhages within the kidney tubules and hypertrophy of the tubular epithelia. Also at these levels of exposure, feminization of male fish could be noticed and parts of males manifested the testis-ova phenomenon. Ovaries of female rare minnow in 25 ng/l EE 2 treatment group were degenerated. Therefore when exposed to EE 2 and NP even at environmental observed concentrations, adverse effects could occur in the reproductive system of adult fishes. The observed hepatic tissue and renal tissue impairment should be due to the induction and accumulation of VTG in organs, especially in males.

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“…Contamination of the water could occur via exposure to xenoestrogens (e.g., from plastic pipe work and tubing [20]) or via exposure to natural oestrogens released by female fish also present in the same water as the males [21]. This may be more apparent when fish are kept at high density with a recirculating water supply (e.g., in stock tanks) or in experiments with slow flow rates and thus long water replacement times [22].…”

Section: Introductionmentioning

confidence: 99%

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Beresford

Brian

Runnalls

et al. 2011

Enviro Toxic and Chemistry

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Abstract-Vitellogenin (VTG) is a precursor of egg-yolk protein and is therefore present at high concentrations in the plasma of female fish. In male fish, VTG concentrations are usually undetectable or low, but can be induced upon exposure to oestrogenic substances either via the water or the diet. This work was carried out to determine the reason for apparently elevated VTG concentrations in unexposed stock male fathead minnow maintained in our laboratory. The results showed clearly that some of the food given to the fish was oestrogenic and that replacement of this with non-oestrogenic food led to a significant reduction in the basal VTG levels measured in male fish after a 6 month period. This reduction in male VTG concentrations drastically increased the sensitivity of the VTG test in further studies carried out with these fish. Moreover, a review of published concentrations of VTG in unexposed male fathead minnow suggests that this problem may exist in other laboratories. The fathead minnow is a standard ecotoxicological fish test species and so these findings will be of interest to any laboratory carrying out fish tests on endocrine disrupting chemicals.

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Development of a Reproductive Performance Test for Endocrine Disrupting Chemicals Using Pair-Breeding Fathead Minnows (Pimephales promelas)

Cited by 205 publications

References 50 publications

Evidence suggesting that di‐n‐butyl phthalate has antiandrogenic effects in fish

Evidence suggesting that di‐n‐butyl phthalate has antiandrogenic effects in fish

Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

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